The optimum pneumoperitoneum pressure for laparoscopic surgery in the rat model

Berguer, Ramón; Cornelius, T.; Dalton, Michelle

doi:10.1007/s004649900486

Cited by 45 publications

(37 citation statements)

References 19 publications

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“…A previous experimental investigation in rats has already revealed impaired strength and wound healing in colocolic anastomosis due to a CO 2 pneumoperitoneum with an IAP of 20 mmHg or higher [13]. However, with respect to the observation that a CO 2 pneumoperitoneum with pressures above 10 mmHg is associated with significant respiratory acidosis in spontaneously breathing rats [15], the transferability of those results to the human situation is vague.…”

Section: Discussionmentioning

confidence: 96%

Impact of pressure and gas type on anastomotic wound healing in rats

Rösch

Stumpf

Junge

et al. 2004

Langenbecks Arch Surg

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Section: Discussionmentioning

confidence: 96%

Impact of pressure and gas type on anastomotic wound healing in rats

Rösch

Stumpf

Junge

et al. 2004

Langenbecks Arch Surg

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“…Therefore, 8 mmHg was used in the present study based on this recent knowledge on the equivalence of Pp pressures between rats and humans. Also, it was demonstrated that intra-abdominal pressures of 10 mmHg or higher cause severe acidosis in rats (18), suggesting that this species is more sensitive to Pp than humans. More information is still necessary to better correlate the intra-abdominal pressures between humans and animal models.…”

Section: Discussionmentioning

confidence: 99%

Does prolonged pneumoperitoneum affect the kidney? Oxidative stress, stereological and electron microscopy study in a rat model

et al. 2013

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“…The second one is the selected value of safe IAP as 12 mm Hg. Although a majority of the studies use various CO 2 pneumoperitoneal pressures, ranging from 10 to 15 mm Hg [32,33,34,35], it has been reported that pressures below 10 mm Hg are more appropriate in a rat model to simulate laparoscopy in humans [36,37]. On the other hand, Sammour et al [38] concluded that pneumoperitoneum-associated oxidative stress is not only due to increased intra-abdominal pressure but also to the use of carbon dioxide as the insufflation gas, and other factors such as the temperature and humidity of the gas may have considerable effects on tissue injury.…”

Section: Discussionmentioning

confidence: 99%

Attenuation of Ischemia/Reperfusion-Induced Ovarian Damage in Rats: Does Edaravone Offer Protection?

Ergenoğlu

Erbaş²,

Akdemir³

et al. 2013

Eur Surg Res

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Aim: The aim of this study was to investigate whether prophylactic treatment with edaravone prevents ischemia/reperfusion (I/R)-induced ovarian damage during pneumoperitoneum in an experimental rat model. Methods: Twenty-eight female Sprague Dawley rats were allocated randomly to 4 groups. The sham group (group 1) was only subjected to catheter insertion, not to pneumoperitoneum. Group 2 received a 1 mg/kg dose of 0.9% sodium chloride by the intraperitoneal route for 10 min before pneumoperitoneum. Groups 3 and 4 received 6 and 12 mg/kg edaravone, respectively, by the intraperitoneal route for 10 min before pneumoperitoneum. After 60 min of pneumoperitoneum, the gas was deflated. Immediately after the reperfusion period, both ovaries were excised for histological scoring, caspase-3 immunohistochemistry and biochemical evaluation including glutathione (GSH) and malondialdehyde (MDA) levels. Also, total antioxidant capacity (TAC) was measured in plasma samples to evaluate the antioxidant effect of edaravone. Results: Ovarian sections in the saline group revealed higher scores for follicular degeneration and edema (p < 0.0001) when compared with the sham group. Administration of different doses of edaravone in rats significantly prevented degenerative changes in the ovary (p < 0.0001). Caspase-3 expression was only detected in the ovarian surface epithelium in all groups, and there was a significant difference between the treatment groups and the saline group (p < 0.0001). Treatment of rats with edaravone reduced caspase-3 expression in a dose-dependent manner. Moreover, biochemical measurements of oxidative stress markers (MDA, GSH and TAC) revealed that prophylactic edaravone treatment attenuated oxidative stress induced by I/R injury. Conclusion: These results indicate that prophylactic treatment with edaravone prevents I/R-induced ovarian damage during pneumoperitoneum in an experimental rat model.

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The optimum pneumoperitoneum pressure for laparoscopic surgery in the rat model

Abstract: CO2 PP pressures above 10 mmHg in rat should be avoided when performing laparoscopic surgery in the rat model.

Cited by 45 publications

References 19 publications

Impact of pressure and gas type on anastomotic wound healing in rats

Impact of pressure and gas type on anastomotic wound healing in rats

Does prolonged pneumoperitoneum affect the kidney? Oxidative stress, stereological and electron microscopy study in a rat model

Attenuation of Ischemia/Reperfusion-Induced Ovarian Damage in Rats: Does Edaravone Offer Protection?

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