Impact of pressure and gas type on anastomotic wound healing in rats

Abstract

A CO(2) pneumoperitoneum with pressures of 3 mmHg and 6 mmHg was found to have no significant impact upon the healing of anastomosis wounds in rats. Helium pneumoperitoneum offers no advantages over CO(2).

Gas-related impact of pneumoperitoneum on systemic wound healing

“…Interestingly, a more recent investigation using an animal model of lipopolysaccharide-induced sepsis even found a protective effect of CO 2 insufflation when compared to helium [28]. As for the gas-related influence on wound healing within the peritoneal cavity, our previous animal experiments revealed no benefit for helium laparoscopy as compared to CO 2 [4,29]. On the contrary, analysing the impact on abdominal wall wound healing, we found a significant increase of collagen I/III ratios after helium laparoscopy [22].…”

“…Whereas no impact on anastomotic wound healing was found, previous investigations in our laboratory revealed beneficial effects for helium pneumoperitoneum on collagen metabolism in laparotomy wounds [4,22].…”

“…In control animals, the abdominal cavity was exposed to room air during 30 min. In all groups, additional twofold ileoileal anastomoses were performed for separate investigations of anastomotic wound healing [4]. The abdominal wall and dorsal skin wound were closed with continuous absorbable 4/0 polyglactin sutures (Vicryl, Ethicon, Hamburg, Germany).…”

“…Animal studies and surgical procedures were performed as described previously [4]. In brief, 54 male SpragueDawley rats weighing 330 g (SD=11 g) were randomized into the following study groups: controls (n=18), CO 2 pneumoperitoneum (n=18) and helium pneumoperitoneum (n=18).…”

“…Other animal experiments showed that CO 2 laparoscopy proved equivalent to open techniques with regard to collagen deposition, contraction rates or tensile strength of intraabdominal wounds [3,4]. Similarly, a variety of clinical studies observed comparable or even fewer wound healing complications after CO 2 laparoscopy in comparison with open surgery [5][6][7][8][9][10][11][12].…”