The optimized research of the in vitro culture of preantral follicles in mice

Wang, Xiyan; Wang, Liguo; Sun, Yanmei; Wu, Di; Li, Jiao; Zhu, Lin; Jiang, Shi‐Wen; Pan, Xiaoyan

doi:10.1002/jcla.23498

Cited by 5 publications

(5 citation statements)

References 34 publications

(104 reference statements)

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“…This method reduces the secondary separation of mechanical follicle stimulation and ensures the structure and function of the follicle. The results showed that follicles separated by mechanical method had a uniform diameter in size, with three to four layers of granulosa cells and intact follicular theca structure, consistent with the reported article [ 10 ].…”

Section: Discussionsupporting

confidence: 91%

“…Brown et al [ 26 ] presumed that preantral follicles began to accept the regulation of hormone levels through vascular construction. In addition, we adopted the mechanical separation method in our experiment [ 10 ]. This method reduces the secondary separation of mechanical follicle stimulation and ensures the structure and function of the follicle.…”

Section: Discussionmentioning

confidence: 99%

“…To date, mouse [ 5 ], bovine [ 6 ], pig [ 7 ], sheep [ 8 ], and human [ 9 ] follicles have successfully acquired mature oocytes. However, the oocytes derived from preantral follicles have unstable in vitro maturation rates (varying from 20% to 70%) [ 10 ]. Furthermore, the culture methods contained fetal bovine serum (FBS), which limited this technology in fertility preservation and application in humans.…”

Section: Introductionmentioning

confidence: 99%

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Optimized study of anin vitro3D culture of preantral follicles in mice

Ren

Zhang

et al. 2023

J Vet Sci

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Background In vitro culture of preantral follicles is a promising technology for fertility preservation. Objectives This study aims to investigate an optimized three-dimensional (3D) fetal bovine serum (FBS)-free preantral follicle culture system having a simple and easy operation. Methods The isolated follicles from mouse ovaries were randomly divided in an ultra-low attachment 96-well plates supplement with FBS or bovine serum albumin (BSA) culture or encapsulated with an alginate supplement with FBS or BSA culture. Meanwhile, estradiol (E 2 ) concentration was assessed through enzyme-linked immunosorbent assay of culture supernatants. The diameter of follicular growth was measured, and the lumen of the follicle was photographed. Spindle microtubules of oocytes were detected via immunofluorescence. The ability of oocytes to fertilize was assessed using in vitro fertilization. Results The diameters were larger for the growing secondary follicles cultured in ultra-low attachment 96-well plates than in the alginate gel on days 6, 8, and 10 ( p < 0.05). Meanwhile, the E 2 concentration in the BSA-supplemented medium was significantly higher in the alginate gel than in the other three groups on days 6 and 8 ( p < 0.05), and the oocytes in the FBS-free system could complete meiosis and fertilization in vitro . Conclusions The present study furnishes insights into the mature oocytes obtained from the 3D culture of the preantral follicle by using ultra-low attachment 96-well plate with an FBS-free system in vitro and supports the clinical practices to achieve competent, mature oocytes for in vitro fertilization.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Optimized study of anin vitro3D culture of preantral follicles in mice

Ren

Zhang

et al. 2023

J Vet Sci

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“…In female animals, FSH stimulates the growth and development of follicles, and increases the oxygen uptake of parietal granulosa cells to promote related protein synthesis [ 13 ]. Especially in the late stage of follicle formation, FSH induces granulosa cells to express a large number of luteinizing hormone receptors and their own proliferation and induces an increase in the expression of epidermal growth factor receptor (EGFR) to promote the occurrence of ovulation under the synergistic effect of LH [ 14 , 15 ]. In addition, FSH treatment up-regulated the synthesis of related hormones, including progesterone [ 16 ].…”

Section: Function and Structure Of Fshmentioning

confidence: 99%

Advances in the Regulation of Mammalian Follicle-Stimulating Hormone Secretion

Wang

Zhang

Yuan

2021

Animals

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Mammalian reproduction is mainly driven and regulated by the hypothalamic-pituitary-gonadal (HPG) axis. Follicle-stimulating hormone (FSH), which is synthesized and secreted by the anterior pituitary gland, is a key regulator that ultimately affects animal fertility. As a dimeric glycoprotein hormone, the biological specificity of FSH is mainly determined by the β subunit. As research techniques are being continuously innovated, studies are exploring the underlying molecular mechanism regulating the secretion of mammalian FSH. This article will review the current knowledge on the molecular mechanisms and signaling pathways systematically regulating FSH synthesis and will present the latest hypothesis about the nuclear cross-talk among the various endocrine-induced pathways for transcriptional regulation of the FSH β subunit. This article will provide novel ideas and potential targets for the improved use of FSH in livestock breeding and therapeutic development.

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“…This interaction between gonadotropin and oocyte paracrine factors is thought to support the physiological mechanism regulating species-specific ovulation rates and fecundity; e.g., in bovines, it is found throughout all follicle development, related to the proliferation and steroidogenesis of granulosa cells [ 135 , 136 ]. In mice, FSH was reported to induce the GCs to express LH receptors and their proliferation in the late stage of follicle formation, and to cause an increase in epidermal growth factor receptor (EGFR) to promote ovulation [ 137 , 138 ].…”

Section: Fsh In the Adult Phase: Folliculogenesis And Spermatogenesismentioning

confidence: 99%

Actions and Roles of FSH in Germinative Cells

Recchia

Jorge

Pessôa

et al. 2021

IJMS

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Follicle stimulating hormone (FSH) is produced by the pituitary gland in a coordinated hypothalamic–pituitary–gonadal (HPG) axis event, plays important roles in reproduction and germ cell development during different phases of reproductive development (fetal, neonatal, puberty, and adult life), and is consequently essential for fertility. FSH is a heterodimeric glycoprotein hormone of two dissociable subunits, α and β. The FSH β-subunit (FSHβ) function starts upon coupling to its specific receptor: follicle-stimulating hormone receptor (FSHR). FSHRs are localized mainly on the surface of target cells on the testis and ovary (granulosa and Sertoli cells) and have recently been found in testicular stem cells and extra-gonadal tissue. Several reproduction disorders are associated with absent or low FSH secretion, with mutation of the FSH β-subunit or the FSH receptor, and/or its signaling pathways. However, the influence of FSH on germ cells is still poorly understood; some studies have suggested that this hormone also plays a determinant role in the self-renewal of germinative cells and acts to increase undifferentiated spermatogonia proliferation. In addition, in vitro, together with other factors, it assists the process of differentiation of primordial germ cells (PGCLCs) into gametes (oocyte-like and SSCLCs). In this review, we describe relevant research on the influence of FSH on spermatogenesis and folliculogenesis, mainly in the germ cell of humans and other species. The possible roles of FSH in germ cell generation in vitro are also presented.

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The optimized research of the in vitro culture of preantral follicles in mice

Abstract: This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

Cited by 5 publications

References 34 publications

Optimized study of anin vitro3D culture of preantral follicles in mice

Optimized study of anin vitro3D culture of preantral follicles in mice

Advances in the Regulation of Mammalian Follicle-Stimulating Hormone Secretion

Actions and Roles of FSH in Germinative Cells

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