The optimization of porous polymeric scaffolds for chondrocyte/atelocollagen based tissue-engineered cartilage

Tanaka, Yoko; Yamaoka, Hisayo; Nishizawa, Satoru; Nagata, Satoru; Ogasawara, Toru; Asawa, Yukiyo; Fujihara, Yuko; Takato, Tsuyoshi; Hoshi, Kazuto

doi:10.1016/j.biomaterials.2010.02.028

Cited by 114 publications

(96 citation statements)

References 34 publications

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“…These cells and growth factors are housed within scaffolds made of a variety of materials. Polypropylene fumarate, 3,4 poly-e-caprolactone (PCL), [5][6][7] polylactic acid, 8,9 and poly(lactic-co-glycolic) acid 10,11 are bioresorbable polymers that have all been investigated, alone or in combination, for bone applications, as have osteoconductive materials such as tricalcium phosphate, 8 hydroxyl apatite, 12 and calcium phosphate. 13,14 This work employs the use of PCL scaffolds seeded with BMP-7-transduced human gingival fibroblasts to study the effects that permeability has on bone tissue regeneration.…”

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confidence: 99%

Effect of Polycaprolactone Scaffold Permeability on Bone Regeneration In Vivo

Mitsak

Kemppainen

Harris

et al. 2011

Tissue Engineering Part A

155

114

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Successful bone tissue engineering depends on the scaffold's ability to allow nutrient diffusion to and waste removal from the regeneration site, as well as provide an appropriate mechanical environment. Since bone is highly vascularized, scaffolds that provide greater mass transport may support increased bone regeneration. Permeability encompasses the salient features of three-dimensional porous scaffold architecture effects on scaffold mass transport. We hypothesized that higher permeability scaffolds will enhance bone regeneration for a given cell seeding density. We manufactured poly-e-caprolactone scaffolds, designed to have the same internal pore design and either a low permeability (0.688 · 10 /N-s), respectively. Scaffolds were seeded with bone morphogenic protein-7-transduced human gingival fibroblasts and implanted subcutaneously in immune-compromised mice for 4 and 8 weeks. Micro-CT evaluation showed better bone penetration into high permeability scaffolds, with blood vessel infiltration visible at 4 weeks. Compression testing showed that scaffold design had more influence on elastic modulus than time point did and that bone tissue infiltration increased the mechanical properties of the high permeability scaffolds at 8 weeks. These results suggest that for polycaprolactone, a more permeable scaffold with regular architecture is best for in vivo bone regeneration. This finding is an important step toward the end goal of optimizing a scaffold for bone tissue engineering.

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mentioning

confidence: 99%

Effect of Polycaprolactone Scaffold Permeability on Bone Regeneration In Vivo

Mitsak

Kemppainen

Harris

et al. 2011

Tissue Engineering Part A

155

114

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“…In a study, it was compared several polylactides and related polymer scaffolds (PLLA and PLGA) administered with a chondrocyte/ atelocollagen mixture and then these scaffolds were implanted subcutaneously in nude mice. After 2 months of implantation all the scaffolds were studied and it was observed that their 3-D shape was maintained throughout the probing period and the higher level for of type I and type II collagen production it was reported in the case of PLLA and PLGA scaffolds [43]. In another study, it was reported a compressive modulus of approximately 6 MPa in PLLA scaffolds with a porous microstructure and it was observed that when PLLA is combined with fibrin gel, the mechanical properties of this complex are increasing and also a higher cell proliferation occurs [44].…”

Section: Synthetic Scaffoldsmentioning

confidence: 94%

Biomaterials for Cartilage Tissue Engineering

Grigore¹

2017

J Tissue Sci Eng

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One of the most challenging issues of musculoskeletal medicine is represented by injuries of the articular cartilage due to the poor regenerative properties of this tissue. A consequence of these injuries is represented by osteoarthritis. Osteoarthritis is the most common chronic condition of the joints, caused because of the progressive wear and tear on articular cartilage. A solution to prevent progressive joint degeneration in osteoarthritis is represented by a surgical intervention which offers the advantage of the success of total joint replacement, but also offers several disadvantages such as such as slower remodeling, immune reaction and disease transmission. In the last years, the researchers have found a solution to avoid surgical intervention by using biomaterials. This study aims to provide an updated survey of the major progress in the flied of biomaterials for cartilage tissue engineering, including biomaterials (natural, synthetic or composites), their advantages or disadvantages and the main seeding cell sources. Also, this review focuses on the progress made in the field of biomaterials for cartilage tissue repair and/or regeneration over the last years.

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“…Then, PLLA is modified or copolymerized with other degradable polymers to reduce degradation time, as shown by the use of radiations to create radicals in the ester alpha carbon which, upon rearrangement, shortens the polymer backbone through the removal of an ester bond and the release of carbon dioxide [100,101]. PLLA is used as bone fixator, scaffold for bone [102,103], cartilage [104], tendon [105], neural [106], and vascular [107] regeneration. Similarly, PDLLA is an amorphous polymer with the random positions of its two isomeric monomers within the polymer chain.…”

Section: Polyestersmentioning

confidence: 99%