The optical properties of two species of marine phytoplankton were characterized by application of principles of reflectance spectroscopy.
Suspensions of Thalassiosira pseudonana from batch cultures and MonochrysisZutheri from continuous cultures were placed in a spectrophotometer for measurements of diffuse spectral transmittance and reflectance. These measurements yielded values for the diffuse absorption coefficiant, a*(h), and diffuse backscattering coefficient, b*(h), two parameters that can be applied to calculations of the effect of phytoplankton crop size on ocean color gpectra. Results show that the absorption and scattering properties of a cell suspension contain information related not only to cell concentration but also to cell age or growth rate. Most important, in batch culture the ratio a*(X):b*(h) decreases with age once the plateau phase has been reached, and in continuous culture the ratio of absorption to backscattering coefficients is roughly proportional to the specific growth rate of the cells. Such changes in a*(A): b*(A) are larger at the red absorption maximum of chlorophyll a than in the blue, where accessory pigments also contribute to the absorption coefficient.