The Optical Design of Probe-type Microscope Objective for Intravital Laser Scanning CARS Microendoscopy

Abstract

A stack of gradient-index (GRIN) rod lenses cannot be used for coherent anti-Stokes Raman scattering (CARS) microendoscopy for insertion to internal organs through a surgical keyhole with minimal invasiveness. That's because GRIN lens has large amount of inherent chromatic aberrations in spite of absolutely requiring a common focus for pump and Stokes beam with each frequency of ωp and ωS. For this endoscopic purpose, we need to develop a long slender probe-type objective, namely probe-type microscope objective (PMO). In this paper, we introduce the structure, the working principle, and the design techniques of PMO which is composed of a probe-type lens module (PLM) and an adaptor lens module (ALM). PLM is first designed for a long slender type and ALM is successively designed by using several design parameters from PLM for eliminating optical discords between scanning unit and PLM. A combined module is optimized again to eliminate some coupling disparities between PLM and ALM for the best PMO. As a result, we can obtain a long slender PMO with perfectly diffraction-limited performance for pump beam of 817 nm and Stokes beam of 1064 nm.

Design and Fabrication of a Multi-modal Confocal Endo-Microscope for Biomedical Imaging

“…An ultra-thin fiber scanning endo-microscope has been also proposed for endoscopic use, but its magnification is very low, so it cannot obtain a high resolution image [6]. Because they have so many advantages, endomicroscopes are being studied all over the world [9]. However, in many cases, it is very difficult to acquire diffraction limited images because the lens in the endo-microscope has to be so small [10].…”

Non-imaging Optical Design of a Measurement Probe for LCD Display Used in a Color Analyzer

“…In order to achieve this purpose, color analyzer needs optical design with non-imaging treatment for gathering and illuminating colored lights within a small area on the sensor [6][7] . While optical designs were mostly reported on imaging optical system [8][9][10] , this study is willing to introduce how to design non-imaging optics by the analytical treatment using Gaussian (or paraxial) optics [11][12][13] . And then an obtained design from the analytical treatment is proceeded to be confirmed and completed by means of commercial software such as CODE V [14] and Light-Tools [15] .…”