Design and Fabrication of a Multi-modal Confocal Endo-Microscope for Biomedical Imaging

Kim, Young-Duk; Ahn, MyoungKi; Gweon, Dae−Gab

doi:10.3807/josk.2011.15.3.300

Cited by 3 publications

(2 citation statements)

References 14 publications

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“…Because grayscale values determine the final depth after development, any errors in the parameters cause considerable losses and negatively affect three-dimensional formability analysis. Thus, many optical lenses require a sample to test the optical quality after design and simulation [17][18][19][20]. A maskless lithography system can substantially reduce the cost and time required for grayscale lithography.…”

Section: Introductionmentioning

confidence: 99%

Forming a Fresnel Zone Lens: Effects of Photoresist on Digital-micromirror-device Maskless Lithography with Grayscale Exposure

Huang

Jeng

2012

Journal of the Optical Society of Korea

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This study discusses photoresist forming using a composite grayscale to fabricate a Fresnel lens. Grayscale lithography is a common production method used to facilitate the forming of lenses with different curvatures and depths. However, this approach is time consuming and expensive. This study proposes a method for overcoming these obstacles by integrating a digital micromirror device and microscope to supplant the traditional physical grayscale mask. This approach provides a simple and practical maskless optical lithography system. According to the results, the two adjacent grayscales displayed substantial differences between the high grayscale and influence the low grayscale that ultimately affected photoresist formation. Furthermore, we show that change of up to 150% in the slope can be achieved by changing the grayscale gradient in the central zone and the ring profile. The results of the optical experiment show a focus change with different gray gradients.

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Section: Introductionmentioning

confidence: 99%

Forming a Fresnel Zone Lens: Effects of Photoresist on Digital-micromirror-device Maskless Lithography with Grayscale Exposure

Huang

Jeng

2012

Journal of the Optical Society of Korea

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“…To achieve these goals, solutions similar to what is widely used on benchtop microscopes can be implemented: multiple excitations in combination with multiple fluorescent markers, targeting multiple biological entities, possibly in interaction. Again, different approaches have been proposed, providing multispectral imaging capabilities, with 23,24 or without 25 microscopic performances.…”

Section: The Need For Multispectral Imagingmentioning

confidence: 99%

Multicolor probe-based confocal laser endomicroscopy: a new world for in vivo and real-time cellular imaging

Vercauteren¹,

Doussoux²,

Cazaux³

et al. 2013

Endoscopic Microscopy VIII

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Since its inception in the field of in vivo imaging, endomicroscopy through optical fiber bundles, or probe-based Confocal Laser Endomicroscopy (pCLE), has extensively proven the benefit of in situ and real-time examination of living tissues at the microscopic scale. By continuously increasing image quality, reducing invasiveness and improving system ergonomics, Mauna Kea Technologies has turned pCLE not only into an irreplaceable research instrument for small animal imaging, but also into an accurate clinical decision making tool with applications as diverse as gastrointestinal endoscopy, pulmonology and urology.The current implementation of pCLE relies on a single fluorescence spectral band making different sources of in vivo information challenging to distinguish. Extending the pCLE approach to multi-color endomicroscopy therefore appears as a natural plan. Coupling simultaneous multi-laser excitation with minimally invasive, microscopic resolution, thin and flexible optics, allows the fusion of complementary and valuable biological information, thus paving the way to a combination of morphological and functional imaging. This paper will detail the architecture of a new system, Cellvizio Dual Band, capable of video rate in vivo and in situ multi-spectral fluorescence imaging with a microscopic resolution. In its standard configuration, the system simultaneously operates at 488 and 660 nm, where it automatically performs the necessary spectral, photometric and geometric calibrations to provide unambiguously co-registered images in real-time. The main hardware and software features, including calibration procedures and sub-micron registration algorithms, will be presented as well as a panorama of its current applications, illustrated with recent results in the field of pre-clinical imaging.

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Microscope autofocus algorithm based on number of image slope variations

Weng

et al. 2021

Opt. Express

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This paper presents a passive autofocus algorithm applicable to interferometric microscopes. The proposed algorithm uses the number of slope variations in an image mask to locate the focal plane (based on focus-inflection points) and identify the two neighboring planes at which fringes respectively appear and disappear. In experiments involving a Mirau objective lens, the proposed algorithm matched the autofocusing performance of conventional algorithms, and significantly outperformed detection schemes based on zero-order interference fringe in dealing with all kinds of surface blemish, regardless of severity. In experiments, the proposed algorithm also proved highly effective in cases without fringes.

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Design and Fabrication of a Multi-modal Confocal Endo-Microscope for Biomedical Imaging

Cited by 3 publications

References 14 publications

Forming a Fresnel Zone Lens: Effects of Photoresist on Digital-micromirror-device Maskless Lithography with Grayscale Exposure

Forming a Fresnel Zone Lens: Effects of Photoresist on Digital-micromirror-device Maskless Lithography with Grayscale Exposure

Multicolor probe-based confocal laser endomicroscopy: a new world for in vivo and real-time cellular imaging

Microscope autofocus algorithm based on number of image slope variations

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