The Ophiostoma piceae complex in the Southern Hemisphere: a phylogenetic study

Beer, Z. Wilhelm de; Wingfield, Brenda D.; Wingfield, Michael J.

doi:10.1017/s0953756203007445

Cited by 44 publications

(60 citation statements)

References 23 publications

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“…These isolates were obtained from Quercus, Acacia and Eucalyptus hosts in Africa, Europe and North America. Their mating type specificities have been determined in previous studies (Brasier and Kirk 1993;De Beer et al 2003;Kamgan et al 2008). For routine cultivation of these isolates, malt-extract agar (MEA; 20 g/L malt extract [Biolab, Merck], 20 g/L agar [Biolab, Merck]) medium and an incubation temperature of 25°C were used.…”

Section: Isolates and Mating Studymentioning

confidence: 93%

“…Most species have a close association with treeinfesting bark beetles and some cause serious tree diseases (Hausner et al 1993). This is true of species in the O. piceae complex (Chung et al 2006;De Beer et al 2003;Harrington et al 2001;Kamgan et al 2008;Uzunovic et al 2000), which includes the Dutch elm disease pathogens (O. ulmi and O. novo-ulmi) that have been responsible for the death of millions of Elm trees in the Northern Hemisphere (Brasier 1990). Other species in the O. piceae complex result in blue-stain of timber and they degrade wood quality.…”

Section: Introductionmentioning

confidence: 99%

“…Other species in the O. piceae complex result in blue-stain of timber and they degrade wood quality. For example, O. quercus is responsible for significant economic losses due to sapstain in hardwoods (De Beer et al 2003;Harrington et al 2001). …”

Section: Introductionmentioning

confidence: 99%

“…Conventional mating tests are laborious and time-consuming as they involve mating all available isolates in every possible combination and subsequent assignment of mating specificities. This traditional approach has been used widely for heterothallic species of Ophiostoma (Brasier and Kirk 1993;De Beer et al 2003;Grobbelaar et al 2009;Harrington et al 2001;Zhou et al 2004). …”

Section: Introductionmentioning

confidence: 99%

“…quercus, a number of mating tester strains is available from previous mating studies (Brasier and Kirk 1993;De Beer et al 2003;Grobbelaar et al 2009;Kamgan et al 2008). The aim of this study was to characterise the MAT genes in some of these O. quercus isolates.…”

Section: Introductionmentioning

confidence: 99%

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Both mating types in the heterothallic fungus Ophiostoma quercus contain MAT1-1 and MAT1-2 genes

et al. 2012

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In heterothallic Ascomycota, two opposite but distinct mating types control all sexual processes. Using mating crosses on agar plates, the heterothallic nature of the wood-inhabiting fungus Ophiostoma quercus was confirmed and mating types were assigned to 10 isolates. Primers were subsequently designed to target both the mating type 1 (MAT1-1) and 2 (MAT1-2) idiomorphs in these isolates. The results showed that all isolates contained sequence fragments representing both idiomorphs. This was unexpected, as each isolate from a heterothallic species would typically contain only one of the two possible MAT idiomorphs. An atypical mating system such as the one described in this study has not previously been encountered in any other Ascomycota mating locus described to date.

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Section: Isolates and Mating Studymentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Both mating types in the heterothallic fungus Ophiostoma quercus contain MAT1-1 and MAT1-2 genes

et al. 2012

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Constant association of ophiostomatoid fungi with the bark beetle Ips subelongatus invading Japanese larch logs

et al. 2009

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Ceratocystiopsis, Ceratocystis, Grosmannia, and Ophiostoma species were isolated from Ips subelongatus and beetle-infested Japanese larch logs collected at several areas in central and northern Honshu Island, Japan, to determine constant associates of I. subelongatus. Ceratocystiopsis minuta, two species of Ceratocystis, three species of Grosmannia, and four species of Ophiostoma were isolated. Of the fungi isolated in the present study, G. laricis, O. brunneociliatum, and O. piceae were constant associates of the beetles. Ceratocystis fujiensis, Ceratocystiopsis minuta, and Ophiostoma sp. F were occasionally isolated with high frequencies of occurrence but were not consistent associates. Ceratocystis fujiensis was most often isolated as the leading fungal invasion in the sapwood of Japanese larch logs invaded by I. subelongatus, confirming that the fungus acts as a primary invader of sapwood in beetle-attacked logs

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Discovery of Ophiostoma tsotsi on Eucalyptus wood chips in China

et al. 2011

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Ophiostoma species such as O. quercus are the most frequent causal agents of sapstain of freshly felled hardwood timber and pulpwood. Many species are regarded as economically important agents of wood degradation. The aim of this study was to identify a collection of Ophiostoma isolates, resembling O. quercus, found on stained Eucalyptus pulpwood chips in China. DNA sequences of the internal transcribed spacer regions, including the 5.8S region, of the ribosomal DNA, and parts of the β-tubulin and elongation factor-1α genes, revealed that the isolates were not O. quercus. Surprisingly, they represented O. tsotsi, a wound-infesting fungus recently described from hardwoods in Africa. In addition, sequence data from an isolate from agarwood in Vietnam, identified in a previous study as belonging to an unknown Pesotum species, were also shown to represent O. tsotsi. A high level of genetic variability was observed among isolates of both O. quercus and O. tsotsi. This was unexpected and suggests that both species have been present in Asia for a significant amount of time.

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The Ophiostoma piceae complex in the Southern Hemisphere: a phylogenetic study

Cited by 44 publications

References 23 publications

Both mating types in the heterothallic fungus Ophiostoma quercus contain MAT1-1 and MAT1-2 genes

Both mating types in the heterothallic fungus Ophiostoma quercus contain MAT1-1 and MAT1-2 genes

Constant association of ophiostomatoid fungi with the bark beetle Ips subelongatus invading Japanese larch logs

Discovery of Ophiostoma tsotsi on Eucalyptus wood chips in China

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