Both mating types in the heterothallic fungus Ophiostoma quercus contain MAT1-1 and MAT1-2 genes

Wilken, P. Markus; Steenkamp, Emma Theodora; Hall, T. A.; Beer, Z. Wilhelm de; Wingfield, Michael J.; Wingfield, Brenda D.

doi:10.1016/j.funbio.2012.01.002

Cited by 19 publications

(20 citation statements)

References 65 publications

(90 reference statements)

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“…We were also able to identify (Coppin et al 1997;Turgeon and Yoder 2000). However, similar structures have also been observed in the MAT1-2 idiomorphs of few other heterothallic Sordariomycetes, where pseudogenes, partial sequences, or homologs of MAT1-1 genes are present in the MAT1-2 idiomorph (Kanematsu et al 2007;Wilken et al 2012;Yokoyama et al 2003).…”

Section: Discussionsupporting

confidence: 77%

“…This is also the first report describing the complete mating type locus in a heterothallic member of the Ophiostomatales. Together with the MAT1-1 idiomorph from G. clavigera (DiGuistini et al 2011), partial MAT1-1 and MAT1-2 idiomorphs from O. novo-ulmi (Jacobi et al 2010;Paoletti et al 2005), partial MAT1-1 and MAT1-2 idiomorphs from O. quercus (Wilken et al 2012), the mating type loci characterized in this study provide insights into the structure and gene orientation of the mating type locus in the Ophiostomatales.…”

Section: Discussionmentioning

confidence: 66%

“…novo-ulmi (Jacobi et al 2010) and O. quercus (Wilken et al 2012) respectively. Alignment of the a domain nucleotide and amino acid sequences with those of other ascomycetes showed a high level of similarity (Fig 2).…”

Section: Cloning Of Mat1-1 In L Profanummentioning

confidence: 99%

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Characterization of the mating-type genes in Leptographium procerum and Leptographium profanum

et al. 2013

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Highlights· The mating-type loci in Leptographium procerum and L. profanum were characterized.· The PCR-based mating-type markers were developed for these two species.· Leptographium procerum and L. profanum had a heterothallic mating system and thus these species are heterothallic. · Cryptic sexual reproduction was suggested for a population of L. procerum. AbstractLeptographium procerum and the closely related species Leptographium profanum, are ascomycetes associated with root-infesting beetles on pines and hardwood trees, respectively.Both species occur in North America where they are apparently native. Leptographium procerum has also been found in Europe, China New Zealand, and South Africa where it has most probably been introduced. As is true for many other Leptographium species, sexual states 2 have never been observed in L. procerum or L. profanum. The objectives of this study were to clone and characterize the mating type loci of these fungi, and to develop markers to determine the mating types of individual isolates. To achieve this, a partial sequence of MAT1-2-1 was amplified using degenerate primers targeting the high mobility group (HMG) sequence. A complete MAT1-2 idiomorph of L. profanum was subsequently obtained by screening a genomic library using the HMG sequence as a probe. Long range PCR was used to amplify the complete MAT1-1 idiomorph of L. profanum and both the MAT1-1 and MAT1-2 idiomorphs of L.procerum. Characterization of the MAT idiomorphs suggests that the MAT genes are fully functional and that individuals of both these species are self-sterile in nature with a heterothallic mating system. Mating type markers were developed and tested on a population of L. procerum isolates from the USA, the assumed center of origin for this species. The results suggest that cryptic sexual reproduction is occurring or has recently taken place within this population.

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Section: Discussionsupporting

confidence: 77%

Section: Discussionmentioning

confidence: 66%

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Characterization of the mating-type genes in Leptographium procerum and Leptographium profanum

et al. 2013

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“…Currently, DNA-based mating studies have been used to evaluate the distribution of the sexual compatibility types of Ophiostoma quercus in different geographical areas (20). According to this antecedent, in the present paper, by PCR of the MAT1 locus, we determined the mating types of 6 autochthonous clinical isolates and 22 native environmental isolates (infected bats and contaminated soil) of H. capsulatum from two distant regions within the Americas (Mexico and Brazil) ( Table 1).…”

Section: Resultsmentioning

confidence: 99%

Frequency and Genetic Diversity of the MAT1 Locus of Histoplasma capsulatum Isolates in Mexico and Brazil

et al. 2013

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The MAT1-1 and MAT1-2 idiomorphs associated with the MAT1 locus of Histoplasma capsulatum were identified by PCR. A total of 28 fungal isolates, 6 isolates from human clinical samples and 22 isolates from environmental (infected bat and contaminated soil) samples, were studied. Among the 14 isolates from Mexico, 71.4% (95% confidence interval [95% CI], 48.3% to 94.5%) were of the MAT1-2 genotype, whereas 100% of the isolates from Brazil were of the MAT1-1 genotype. Each MAT1 idiomorphic region was sequenced and aligned, using the sequences of the G-217B (؉ mating type) and G-186AR (؊ mating type) strains as references. BLASTn analyses of the MAT1-1 and MAT1-2 sequences studied correlated with their respective ؉ and ؊ mating type genotypes. Trees were generated by the maximum likelihood (ML) method to search for similarity among isolates of each MAT1 idiomorph. All MAT1-1 isolates originated from Brazilian bats formed a well-defined group; three isolates from Mexico, the G-217B strain, and a subgroup encompassing all soil-derived isolates and two clinical isolates from Brazil formed a second group; last, one isolate (EH-696P) from a migratory bat captured in Mexico formed a third group of the MAT1-1 genotype. The MAT1-2 idiomorph formed two groups, one of which included two H. capsulatum isolates from infected bats that were closely related to the G-186AR strain. The other group was formed by two human isolates and six isolates from infected bats. Concatenated ML trees, with internal transcribed spacer 1 (ITS1) -5.8S-ITS2 and MAT1-1 or MAT1-2 sequences, support the relatedness of MAT1-1 or MAT1-2 isolates. H. capsulatum mating types were associated with the geographical origin of the isolates, and all isolates from Brazil correlated with their environmental sources.

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“…In contrast, individual strains of homothallic ascomycetes contain both the MAT1-1 and MAT1-2 idiomorphs in their genomes and they are, therefore, self-fertile. While this is generally true, there are some exceptions, such as in the case of Ophiostoma quercus (Wilken et al, 2012).…”

Section: Introductionmentioning

confidence: 97%

Mating type markers reveal high levels of heterothallism in Leptographium sensu lato

et al. 2016

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Species of Leptographium sensu lato (Ophiostomatales, Ascomycetes) are sap-stain fungi vectored by bark beetles (Coleoptera, Scolytinae) and some species cause or are associated with tree diseases. Sexual states have been reported for more than 30 species in this group and these have been treated in the sexual genus Grosmannia. No sexual state is known for at least 59 additional species and these reside in the genus Leptographium. The discovery of sexual states for species of Leptographium relies mainly on the presence of fruiting bodies on host tissue at the time of isolation and/or intensive laboratory mating studies, which commonly have a low levels of success. In this study, markers were developed to diagnose mating-type and to study sexual compatibility of species in Leptographium sensu lato using these markers. To achieve this objective, available mating type sequences for species of Leptographium sensu lato and Ophiostoma were obtained, aligned and used to design primers to amplify MAT genes in Grosmannia and Leptographium species. Using these primers, it was possible to amplify portions of the mating type genes for 42 species and to determine thallism, in many species for the first time. Surprisingly, the results showed that heterothallic and putatively heterothallic species are abundant (39 out of 42 species) in Leptographium sensu lato, and only three species were confirmed to be homothallic. The mating-type markers developed in this study will be useful for future studies concerning mating type and sexual compatibility of species in this genus.

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Both mating types in the heterothallic fungus Ophiostoma quercus contain MAT1-1 and MAT1-2 genes

Cited by 19 publications

References 65 publications

Characterization of the mating-type genes in Leptographium procerum and Leptographium profanum

Characterization of the mating-type genes in Leptographium procerum and Leptographium profanum

Frequency and Genetic Diversity of the MAT1 Locus of Histoplasma capsulatum Isolates in Mexico and Brazil

Mating type markers reveal high levels of heterothallism in Leptographium sensu lato

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