The Ongoing Revolution of MALDI-TOF Mass Spectrometry for Microbiology Reaches Tropical Africa

Fall, B.; Lo, Cheikh Ibrahima; Samb-Ba, Bissoume; Perrot, Nadine; Diawara, Silman; Gueye, Mamadou Wagué; Sow, Kowry; Aubadie-Ladrix, Maxence; Mediannikov, Oleg; Sokhna, Cheikh; Diémé, Yaya; Chatellier, Sonia; Wade, B.; Raoult, Didier; Fénollar, Florence

doi:10.4269/ajtmh.14-0406

Cited by 43 publications

(34 citation statements)

References 40 publications

(72 reference statements)

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“…Identification systems validated with bacterial collections obtained in high-resource settings Identification methods not validated with bacterial collections from low-resource settings; 49,50 some tropical bacteria cannot be reliably identified with commercial identification methods 51,52 Antimicrobial susceptibility testing of bacteria…”

Section: Identificationmentioning

confidence: 99%

Clinical bacteriology in low-resource settings: today's solutions

Ombelet

Ronat

Walsh

et al. 2018

The Lancet Infectious Diseases

137

159

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Low-resource settings are disproportionately burdened by infectious diseases and antimicrobial resistance. Good quality clinical bacteriology through a well functioning reference laboratory network is necessary for effective resistance control, but low-resource settings face infrastructural, technical, and behavioural challenges in the implementation of clinical bacteriology. In this Personal View, we explore what constitutes successful implementation of clinical bacteriology in low-resource settings and describe a framework for implementation that is suitable for general referral hospitals in low-income and middle-income countries with a moderate infrastructure. Most microbiological techniques and equipment are not developed for the specific needs of such settings. Pending the arrival of a new generation diagnostics for these settings, we suggest focus on improving, adapting, and implementing conventional, culture-based techniques. Priorities in low-resource settings include harmonised, quality assured, and tropicalised equipment, consumables, and techniques, and rationalised bacterial identification and testing for antimicrobial resistance. Diagnostics should be integrated into clinical care and patient management; clinically relevant specimens must be appropriately selected and prioritised. Open-access training materials and information management tools should be developed. Also important is the need for onsite validation and field adoption of diagnostics in low-resource settings, with considerable shortening of the time between development and implementation of diagnostics. We argue that the implementation of clinical bacteriology in low-resource settings improves patient management, provides valuable surveillance for local antibiotic treatment guidelines and national policies, and supports containment of antimicrobial resistance and the prevention and control of hospital-acquired infections.

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Section: Identificationmentioning

confidence: 99%

Clinical bacteriology in low-resource settings: today's solutions

Ombelet

Ronat

Walsh

et al. 2018

The Lancet Infectious Diseases

137

159

View full text Add to dashboard Cite

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“…The upfront equipment cost necessary for a MALDI-TOF MS system may be higher than that of a fluorescent system, but the cost is lowering to that point that when combined with the low cost and high speed of sample analysis, MALDI-TOF systems are beginning to be adopted in high and low resource countries (Fall, et al, 2015, Martiny, et al, 2014, Neville, et al, 2011). MALDI-TOF MS analysis offers several advantages for our detection scheme.…”

Section: Discussionmentioning

confidence: 99%

Phage-protease-peptide: a novel trifecta enabling multiplex detection of viable bacterial pathogens

Alcaine

Tilton

Serrano

et al. 2015

Appl Microbiol Biotechnol

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Bacteriophages represent rapid, readily targeted, and easily produced molecular probes for the detection of bacterial pathogens. Molecular biology techniques have allowed researchers to make significant advances in the bioengineering of bacteriophage to further improve speed and sensitivity of detection. Despite their host-specificity, bacteriophages have not been meaningfully leveraged in multiplex detection of bacterial pathogens. We propose a proof-of-principal phage-based scheme to enable multiplex detection. Our scheme involves bioengineering bacteriophage to carry a gene for a specific protease, which is expressed during infection of the target cell. Upon lysis, the protease is released to cleave a reporter peptide, and the signal detected. Here we demonstrate the successful: i) modification of T7 bacteriophage to carry TEV protease; ii) expression of TEV protease by E. coli following infection by our modified T7, an average of 2000 units of protease per phage are produced during infection; and iii) proof-of-principle detection of E. coli in 3 hours after a primary enrichment via TEV protease activity using a fluorescent peptide and using a designed target peptide for MALDI-TOF MS analysis. This proof-of-principle can be translated to other phage-protease-peptide combinations to enable multiplex bacterial detection, and readily adopted on multiple platforms, like MALDI-TOF MS or fluorescent readers, commonly found in labs.

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“…Thus, for most resource poor countries this is not an option. Fall et al (45), presented the use of MALDI-TOF in Dakar (Senegal) where it was successfully used to identify species causing infectious diseases in tropical Africa.…”

Section: Towards Multiparameter Testingmentioning

confidence: 99%

Improving diagnosis of pneumococcal disease by multiparameter testing and micro/nanotechnologies

Salieb-Beugelaar¹,

Zhang

Nigo³

et al. 2016

European Journal of Nanomedicine

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The diagnosis and management of pneumococcal disease remains challenging, in particular in children who often are asymptomatic carriers, and in low-income countries with a high morbidity and mortality from febrile illnesses where the broad range of bacterial, viral and parasitic cases are in contrast to limited, diagnostic resources. Integration of multiple markers into a single, rapid test is desirable in such situations. Likewise, the development of multiparameter tests for relevant arrays of pathogens is important to avoid overtreatment of febrile syndromes with antibiotics. Miniaturization of tests through use of micro-and nanotechnologies combines several advantages: miniaturization reduces sample requirements, reduces the use of consumables and reagents leading to a reduction in costs, facilitates parallelization, enables point-of-care use of diagnostic equipment and even reduces the amount of potentially infectious disposables, characteristics that are highly desirable in most healthcare settings. This critical review emphasizes our vision on the importance of multiparametric testing for diagnosing pneumococcal infections in patients with fever and examines recent relevant developments in micro/nanotechnologies to achieve this goal.

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The Ongoing Revolution of MALDI-TOF Mass Spectrometry for Microbiology Reaches Tropical Africa

Cited by 43 publications

References 40 publications

Clinical bacteriology in low-resource settings: today's solutions

Clinical bacteriology in low-resource settings: today's solutions

Phage-protease-peptide: a novel trifecta enabling multiplex detection of viable bacterial pathogens

Improving diagnosis of pneumococcal disease by multiparameter testing and micro/nanotechnologies

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