The on-line preconcentration of nanoparticles in electromigration techniques

Dziomba, Szymon; Ciura, Krzesimir; Dawid, Marta

doi:10.1016/j.chroma.2019.06.053

Cited by 5 publications

(5 citation statements)

References 71 publications

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“…While simple extension of injection time might lead to sample overloading and peaks broadening, a number of online preconcentration strategies was developed [15,16]. However, implementation of certain online preconcentration techniques in the case of biological particles is challenging due to their stability and integrity issues during focusing process [17]. This is why the present work was focused on the CZE method that utilize standard sample injection.…”

Section: Resultsmentioning

confidence: 99%

“…The migration of particle under electric field might be suppressed due to the conductance of electric double layer (EDL). This phenomenon is known as relaxation effect and is dependent on the relative size of EDL to physical diameter of the particle [17,29]. While the physical diameter of certain EVs might be considered constant, the EDL size differs depending on the buffer physicochemical properties like ionic strength and counter‐ions type [32].…”

Section: Resultsmentioning

confidence: 99%

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Investigation of selected parameters of capillary zone electrophoresis method for analysis of isolates of outer membrane vesicles

et al. 2021

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The capillary zone electrophoresis (CZE) has recently been proposed by our group as a novel technique for outer membrane vesicles (OMVs) characterization (J. Chromatography 1621 (2020) 461047). In present work the impact of selected parameters of CZE method on OMVs isolates analysis was assessed. It was shown that the extension of sample injection plug length significantly improves the detectability of macromolecular aggregates in CZE. Moreover, a negligible adsorption of OMVs to both uncoated and polymer‐modified (poly(DMA‐GMA‐MAPS)) capillary walls was proven. Finally, the relaxation effect as well as deformation/polarization of vesicles were demonstrated to affect OMVs electrophoretic mobility. The significance of these findings was discussed.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Investigation of selected parameters of capillary zone electrophoresis method for analysis of isolates of outer membrane vesicles

et al. 2021

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“…Therefore, many ion sources have been developed to achieve this transition. The most commonly used ionization technique for liquid samples is ESI [5–49]. The integrated two‐phase electroextraction (EE), CE, and MS combine rapid sample extraction to enable high‐performance separation and sensitive detection of metabolites (i.e., leucine, isoleucine, adenosine, phenylalanine, and guanosine).…”

Section: Strategies For Sensitivity Enhancement In Cementioning

confidence: 99%

“…In reversed polarity, the setup and the EOF direction are opposite allowing better separation for certain analytes such as anions. Besides, polarity switching is applied within the stacking technique [19,20] to obtain better separation and higher sensitivity. The gained signal enhancement was more than 200-fold by the stacking technique [16,17,21,22].…”

Section: Introductionmentioning

confidence: 99%

Sensitivity enhancement in capillary electrophoresis and their applications for analyses of pharmaceutical and related biochemical substances

Suntornsuk

Anurukvorakun

2022

Electrophoresis

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This review aims to illustrate sensitivity enhancement methods in capillary electrophoresis (CE) and their applications for pharmaceutical and related biochemical substance analyses. The first two parts of the article describe the introduction and principle of CE. The main part focuses on strategies for sensitivity improvement in CE including detector and capillary technologies and preconcentration techniques. Applications of these techniques for pharmaceutical and biomedical substance analyses are surveyed during the years 2018-2021.

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“…As detection sensitivity is always limited with reported CE-UV or CE-MS approaches, some specific modes of on-line preconcentration are often needed prior to electrophoretic separations of nanoparticles [5]. In the case of core-shell magnetic nanoparticles (CSMNPs) that have been exploited in different health-care applications (i.e.…”

Section: ) Introductionmentioning

confidence: 99%

Electroosmotic flow modulation for improved electrokinetic preconcentration: Application to capillary electrophoresis of fluorescent magnetic nanoparticles

Nguyen

Smadja

Taverna

et al. 2021

Analytica Chimica Acta

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It is reported in this study a new approach for modulation and even suppression of the electroosmotic flow (EOF) to achieve better electrokinetic preconcentration in capillary electrophoresis This is based on the augmentation of the buffer's concentrations to very high levels (more than a thousand of mM) without recourse to any dynamic/permanent coating nor viscous gel. The use of large weakly charged molecules as background electrolyte's constituents allows working at extreme concentration ranges without penalty of high electric currents and Joule heating. By this way, the electroosmotic mobility could be modulated over a wide range (2 -60 × 10 -5 cm 2 .V -1 .s -1 under alkaline conditions), and suppressed to levels equivalent to those obtained with several neutral coatings. For demonstration, this new approach was applied for sensitive determination of core-shell magnetic nanoparticles (CSMNPs) having high potential for healthcare applications such as imaging agents for diagnostics and controllable cargos for nanomedicine. Different profiles were achieved for purposemade and commercial magnetic nanoparticles using CE coupled with light-emitting-diode induced fluorescence (LEDIF) detection. Compared to the conventional capillary electrophoresis (CE-UV) method for characterization of magnetic nanoparticles, our proposed approach with fluorescent detection and EOF-assisted preconcentration offers almost 350-fold sensitivity improvement. Furthermore, our scheme can be used for monitoring the interaction between CSMNPs and target pharmaceutical molecules, serving for drug delivery development. A preliminary study with two antibiotics using this approach revealed that kanamycin interacts better with the target nanoparticles than amikacin.

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The on-line preconcentration of nanoparticles in electromigration techniques

Cited by 5 publications

References 71 publications

Investigation of selected parameters of capillary zone electrophoresis method for analysis of isolates of outer membrane vesicles

Investigation of selected parameters of capillary zone electrophoresis method for analysis of isolates of outer membrane vesicles

Sensitivity enhancement in capillary electrophoresis and their applications for analyses of pharmaceutical and related biochemical substances

Electroosmotic flow modulation for improved electrokinetic preconcentration: Application to capillary electrophoresis of fluorescent magnetic nanoparticles

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