Sensitivity enhancement in capillary electrophoresis and their applications for analyses of pharmaceutical and related biochemical substances

Suntornsuk, Leena; Anurukvorakun, Oraphan

doi:10.1002/elps.202100236

Cited by 17 publications

(12 citation statements)

References 151 publications

(257 reference statements)

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“…On the other hand, sweeping is based on focusing the analytes as narrow bands using pseudostationary phases such as micelles [49]. The interaction between the analyte and the pseudophase controls its efficiency in sensitivity enhancement.…”

Section: Sample Preparationmentioning

confidence: 99%

Recent advances in microchip electrophoresis for analysis of pathogenic bacteria and viruses

et al. 2022

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Life‐threatening diseases, such as hepatitis B, pneumonia, tuberculosis, and COVID‐19, are widespread due to pathogenic bacteria and viruses. Therefore, the development of highly sensitive, rapid, portable, cost‐effective, and selective methods for the analysis of such microorganisms is a great challenge. Microchip electrophoresis (ME) has been widely used in recent years for the analysis of bacterial and viral pathogens in biological and environmental samples owing to its portability, simplicity, cost‐effectiveness, and rapid analysis. However, microbial enrichment and purification are critical steps for accurate and sensitive analysis of pathogenic bacteria and viruses in complex matrices. Therefore, we first discussed the advances in the sample preparation technologies associated with the accurate analysis of such microorganisms, especially the on‐chip microfluidic‐based sample preparations such as dielectrophoresis and microfluidic membrane filtration. Thereafter, we focused on the recent advances in the lab‐on‐a‐chip electrophoretic analysis of pathogenic bacteria and viruses in different complex matrices. As the microbial analysis is mainly based on the analysis of nucleic acid of the microorganism, the integration of nucleic acid‐based amplification techniques such as polymerase chain reaction (PCR), quantitative PCR, and multiplex PCR with ME will result in an accurate and sensitive analysis of microbial pathogens. Such analyses are very important for the point‐of‐care diagnosis of various infectious diseases.

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Section: Sample Preparationmentioning

confidence: 99%

Recent advances in microchip electrophoresis for analysis of pathogenic bacteria and viruses

et al. 2022

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“…Dünyada artan jenerik ilaç endüstrisi pazarı nedeniyle, bu ilaçların tayini için analitik metotların geliştirilmesi gereklidir. Yapılan literatür taramasında pravastatinin tayini için yüksek 146 performans sıvı kromatografi (HPLC) [3][4][5], kapiler elektroforez [6], elektroanalitik yöntemler [7][8] yaygın şekilde kullanılmaktadır. Bu yöntemler arasında ilaç endüstrisinde yaygın şekilde kullanılan HPLC yöntemi, pravastatinin kalitatif ve kantitatif tayini için tercih edilmektedir.…”

Section: Introductionunclassified

Pravastatinin Su ve Su-Asetonitril İkili Karışımlarında İyonlaşma Sabiti Değerinin RPLC Metoduyla Belirlenmesi

TANRIKULU

KONÇE

Demıralay

2023

Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi

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Ters faz sıvı kromatografi (RPLC) yöntemi asidik ve bazik fonksiyonel grup içeren bileşiklerin iyonlaşma/protonasyon (pKa) sabitlerinin tayini için tercih edilmektedir. pKa değeri, ilaçların absorpsiyon, dağılım, metabolizma, atılım, toksitite (ADMET) özelliklerini tahmin etmeye yarayan önemli bir parametredir. Sunulan bu çalışmada, pravastatinin su-asetonitril ikili karışımında pKa değerinin tayini için RPLC metot geliştirilmiş ve asetonitrilin mobil fazdaki miktarının değişimiyle pravastatinin kromatografik davranışı da belirlenmiştir. Çalışma Nücleosil C4 (250×4,6 mm, 5 µm) kolonda, 37°C’de ve 1 mL/dakika sabit akış hızında gerçekleştirilmiştir. Elde edilen alıkonma verileriyle doğrusal olmayan model kullanılarak bileşiğin pKa değeri belirlenmiştir. Hidrofilik özellikte olan pravastatinin su ortamındaki pKa değeri farklı organik modifiyer-su karışımlarında tayin edilen mobil faz pKa değerleri ve asetonitrilin makroskopik sabitleri (mol kesri, dielektrik sabiti, vs.), çözücü-çözünen arasındaki spesifik solvasyon etkileşim parametreleri kullanılarak hesaplanmıştır. Hesaplanan bu önemli fizikokimyasal parametrenin literatür verileriyle uyumlu olduğu görülmüştür.

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“…As a rapidly developing analytical technique, CE has been widely used in the field of pharmaceutical analysis because of its low samples and organic reagents consumption, high efficiency and sensitivity, simple equipment, and wide applicability [20][21][22][23]. Therein, MEKC is usually used as a common mode because of its good characteristic of separation.…”

Section: Introductionmentioning

confidence: 99%

In‐capillary screening and quantifying the antioxidants of Yangxinshi Tablet using field‐enhanced sample injection‐micellar electrokinetic chromatography

Zhou

Wang

Liu

et al. 2023

J of Separation Science

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Award Number: 21ZYJDJC00080An in-capillary 2,2′-azinobis-(3-ethylbenzthiazoline-6-sulphonate) as oxyradicals combining field-enhanced sample injection with micellar electrokinetic chromatography was developed for screening and determination of the major antioxidants in Yangxinshi Tablet. To obtain simultaneous separation and detection of radicals and analytes, relevant factors were optimized separately. Under the optimum conditions, four compounds including salvianolic acid B, hyperoside, puerarin, and caffeic acid were identified as the major antioxidants. All validation results covering recovery, precision, and stability demonstrated good applicability of the method. On this basis, the total antioxidant activity was successfully evaluated in terms of the decreased peak area of radicals. There was a correlation coefficient of 0.8974 between the total contents of major antioxidants and the total antioxidative activity of the sample. Therefore, these four compounds were selected as combinatorial markers for the quality evaluation of Yangxinshi Tablet. It was concluded that the established method presented a powerful potential to screen and quantify active ingredients in the complex preparation of Chinese medicine.

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Sensitivity enhancement in capillary electrophoresis and their applications for analyses of pharmaceutical and related biochemical substances

Cited by 17 publications

References 151 publications

Recent advances in microchip electrophoresis for analysis of pathogenic bacteria and viruses

Recent advances in microchip electrophoresis for analysis of pathogenic bacteria and viruses

Pravastatinin Su ve Su-Asetonitril İkili Karışımlarında İyonlaşma Sabiti Değerinin RPLC Metoduyla Belirlenmesi

In‐capillary screening and quantifying the antioxidants of Yangxinshi Tablet using field‐enhanced sample injection‐micellar electrokinetic chromatography

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