The odontoblasts and the blood capillaries in the incisal region of rat incisor pulp.

Shoji, Tetsuo; Semba, Teruhiko

doi:10.2330/joralbiosci1965.32.226

Cited by 3 publications

(1 citation statement)

References 5 publications

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“…The intense expression of Dsp mRNA in mature odontoblasts in this study was precisely correlated with their functional activity. In contrast, the term "post-odontoblasts" is not suitable for the general terminology used in the process of dentinogenesis, because their appearance is a peculiar status due to an inflammatory reaction caused by the attrition of the incisal tip (39,50,52). The expression of Dsp mRNA in the odontoblasts became weak in intensity at Week 5 and almost disappeared at Week 8 in this study, suggesting the existence of odontoblasts that have vacated an active synthetic phase and committed to a quiescent phase.…”

Section: Classification Of Odontoblast-lineage Cells (Table 3)mentioning

confidence: 99%

Expression patterns of nestin and dentin sialoprotein during dentinogenesis in mice

et al. 2012

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Differentiated odontoblasts could not be identified by one unique phenotypic marker, but the combination of expression of dentin phosphoprotein (Dpp), dentin sialoprotein (Dsp), dentin matrix protein 1 (Dmp1), and nestin may be valuable for the assessment of these cells. However, the findings using these proteins remain controversial. This study aimed to compare two odontoblast differentiation markers: nestin and Dsp in the process of dentinogenesis in mice. We performed immunohistochemistry and/or in situ hybridization technique for nestin and Dsp using 3-week-old incisors as well as postnatal 1-day-to 8-week-old molars. Preodontoblasts began to express nestin and Dsp proteins and Dsp mRNA, which increased in their intensity according to the progress of odontoblast differentiation in both incisors and developing molars. Nestin was consistently expressed in the differentiated odontoblasts even after the completion of dentin matrix deposition. The expression of Dsp mRNA coincided with the odontoblast secretory activity for dentin matrix deposition. In contrast, other pulpal cells, predentin matrix and dentinal tubules also showed a positive reaction for Dsp protein in addition to differentiated odontoblasts. In conclusion, nestin is valuable as a differentiation marker for odontoblasts, whereas Dsp mRNA is a functional marker for their secretory activity.Dentin is a hard connective tissue that forms the bulk of the tooth, and it is a bone-like matrix characterized by multiple closely packed dentinal tubules that traverse its entire thickness and contain the cytoplasmic processes of odontoblasts, which are responsible for the formation and maintenance of the dentin. The cell bodies of the odontoblasts are aligned along the inner aspect of the dentin, beneath a layer of predentin, where they also form the peripheral boundary of the dental pulp (35). The odontoblasts are terminally differentiated ectomesenchymal cells that synthesize several collagenous and non-collagenous proteins (NCPs) (45). The morphologically discernible differentiation of the odontoblast begins with the dental papilla cells adjacent to the inner enamel epithelium (7). The dental papilla cells adjoining the acellular zone rapidly enlarge and elongate to become preodontoblasts first and then odontoblasts as their cytoplasm increases in volume to contain increasing amounts of proteinsynthesizing organelles. The morphology of odontoblasts reflects their functional activity and ranges from an active synthetic phase to a quiescent phase (35). However, the preferred use of terminology regarding the classification of odontoblasts is controversial, because several terms for differentiated odontoblasts have been used by different researchers, i.e., secretory, transitional, and aged odontoblasts (12), young and old odontoblasts (51), or

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Section: Classification Of Odontoblast-lineage Cells (Table 3)mentioning

confidence: 99%

Expression patterns of nestin and dentin sialoprotein during dentinogenesis in mice

et al. 2012

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Distribution and organization of peripheral capillaries in dental pulp and their relationship to odontoblasts

Yoshida

Ohshima

1996

Anat. Rec.

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The relationship between odontoblasts and pulp capillaries in the process of enamel- and cementum-related dentin formation in rat incisors

Ohshima

Yoshida

1992

Cell Tissue Res

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The relationship between odontoblasts and pulp capillaries in the process of dentinogenesis was studied in rat lower incisors, both on the labial and lingual sides, using light and transmission electron microscopy. The odontoblasts showed remarkable differences from the apical to the incisal end. Near the apical end of the tooth, "immature odontoblasts", which were thought to be involved in the formation of the mantle dentin, were arranged in a single layer, and continuous capillaries were located just beneath the odontoblasts. In the middle of the tooth, "mature odontoblasts" with highly developed cell organelles and notable processes formed a pseudostratified layer; fenestrated capillaries were found between these cells close to the predentin. The height of the odontoblast layer and the rate of dentin deposition on the labial (enamel-related) side was significantly greater than that on the lingual (cementum-related) side. Near the incisal end, cementum-related odontoblasts gradually decreased in height and number to become "post-odontoblasts" that produced atubular dentin; continuous capillaries were located subjacent to the post-odontoblasts. On the labial (enamel-related) side, however, odontoblasts retained their pseudostratification; fenestrated capillaries were still observed in the odontoblast layer. No atubular dentin was formed on the labial side.

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The odontoblasts and the blood capillaries in the incisal region of rat incisor pulp.

Abstract: The odontoblasts and the blood capillaries in the incisal region of the rat incisor pulp were

Cited by 3 publications

References 5 publications

Expression patterns of nestin and dentin sialoprotein during dentinogenesis in mice

Expression patterns of nestin and dentin sialoprotein during dentinogenesis in mice

Distribution and organization of peripheral capillaries in dental pulp and their relationship to odontoblasts

The relationship between odontoblasts and pulp capillaries in the process of enamel- and cementum-related dentin formation in rat incisors

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