Distribution and organization of peripheral capillaries in dental pulp and their relationship to odontoblasts

Yoshida, Shigemitsu; Ohshima, Hayato

doi:10.1002/(sici)1097-0185(199606)245:2<313::aid-ar14>3.0.co;2-s

Cited by 51 publications

(27 citation statements)

References 47 publications

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“…The present study clearly demonstrated that the expression of Dsp mRNA coincided with the odontoblast secretory activity for dentin matrix deposition in both 3-week-old incisors and developing molars, judging from the previous data: the rate of dentin deposition in the medial coronal portion almost ceases after 60 days in rats (57) and the progress of dentinogenesis in mice is faster than that in rats (26,27). In contrast, other pulpal cells, predentin matrix and dentinal tubules showed positive reactions only for Dsp protein and increased in their intensity according to the progress of tooth development.…”

Section: Dspsupporting

confidence: 80%

Expression patterns of nestin and dentin sialoprotein during dentinogenesis in mice

et al. 2012

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Differentiated odontoblasts could not be identified by one unique phenotypic marker, but the combination of expression of dentin phosphoprotein (Dpp), dentin sialoprotein (Dsp), dentin matrix protein 1 (Dmp1), and nestin may be valuable for the assessment of these cells. However, the findings using these proteins remain controversial. This study aimed to compare two odontoblast differentiation markers: nestin and Dsp in the process of dentinogenesis in mice. We performed immunohistochemistry and/or in situ hybridization technique for nestin and Dsp using 3-week-old incisors as well as postnatal 1-day-to 8-week-old molars. Preodontoblasts began to express nestin and Dsp proteins and Dsp mRNA, which increased in their intensity according to the progress of odontoblast differentiation in both incisors and developing molars. Nestin was consistently expressed in the differentiated odontoblasts even after the completion of dentin matrix deposition. The expression of Dsp mRNA coincided with the odontoblast secretory activity for dentin matrix deposition. In contrast, other pulpal cells, predentin matrix and dentinal tubules also showed a positive reaction for Dsp protein in addition to differentiated odontoblasts. In conclusion, nestin is valuable as a differentiation marker for odontoblasts, whereas Dsp mRNA is a functional marker for their secretory activity.Dentin is a hard connective tissue that forms the bulk of the tooth, and it is a bone-like matrix characterized by multiple closely packed dentinal tubules that traverse its entire thickness and contain the cytoplasmic processes of odontoblasts, which are responsible for the formation and maintenance of the dentin. The cell bodies of the odontoblasts are aligned along the inner aspect of the dentin, beneath a layer of predentin, where they also form the peripheral boundary of the dental pulp (35). The odontoblasts are terminally differentiated ectomesenchymal cells that synthesize several collagenous and non-collagenous proteins (NCPs) (45). The morphologically discernible differentiation of the odontoblast begins with the dental papilla cells adjacent to the inner enamel epithelium (7). The dental papilla cells adjoining the acellular zone rapidly enlarge and elongate to become preodontoblasts first and then odontoblasts as their cytoplasm increases in volume to contain increasing amounts of proteinsynthesizing organelles. The morphology of odontoblasts reflects their functional activity and ranges from an active synthetic phase to a quiescent phase (35). However, the preferred use of terminology regarding the classification of odontoblasts is controversial, because several terms for differentiated odontoblasts have been used by different researchers, i.e., secretory, transitional, and aged odontoblasts (12), young and old odontoblasts (51), or

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Section: Dspsupporting

confidence: 80%

Expression patterns of nestin and dentin sialoprotein during dentinogenesis in mice

et al. 2012

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“…During the bell stage of tooth development, the endothelial cells rapidly colonise the dental papilla reaching up to the odontoblast layer. The close relationship between the odontoblasts and peripheral capillaries of the tooth has been related to the secretory activity of the odontoblasts (Yoshida and Ohshima 1996).…”

Section: Discussionmentioning

confidence: 99%

Contribution of mesoderm to the developing dental papilla

Rothová

Feng

Sharpe³

et al. 2011

Int. J. Dev. Biol.

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Teeth develop from epithelium and neural crest-derived mesenchyme via a series of reciprocal epithelial-mesenchymal interactions. The majority of the dental papilla of the tooth has been demonstrated to be of neural crest origin. However, non-neural crest cells have also been observed in this region from the bud stage of tooth development onwards. The number of these non-neural crest-derived cells rises as the dental papilla develops. However, their origin is unknown. We have followed migration of cells into the tooth in vitro using DiI to fate map regions surrounding the developing tooth. To identify the contribution of mesodermally-derived cells, we have utilised Mesp1cre/R26R transgenic reporter mice. We document that cells outside the early tooth primordium migrate into the developing dental papilla from the late cap stage of development. Here, we show that migrating cells are mesodermally-derived and create a network of endothelial cells, forming the blood vessels of the tooth. No cells of mesodermal origin were present in the condensed mesenchyme surrounding the dental epithelium until the cap stage of tooth development. Mesodermally-derived cells start invading the dental papilla at the late cap stage, providing the blood supply to the dental pulp. Endothelial cells are able to invade the developing dental papilla in vitro using the slice culture method. Understanding the origin and timing of migration of the mesodermally-derived cells is an important advance in our understanding of how a tooth develops and is particularly relevant to studies which aim to create bioengineered teeth.

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“…In addition, recently a meticulous in vitro study elucidated PTHR1 subcellular trafficking after its internalization, and has shown the Golgi apparatus to be the final destination of this trafficking system (Tawfeek and Abou-Samra, 2004). Here, PTHR1 intensive positive reactions were observed at the juxtanuclear compartment of columnar odontoblasts and ameloblasts known as the Golgi area (Bishop and Boyde, 1986;Dahl, 1993;Ohshima, and Yoshida, 1992;Sakaki and Garant, 1996;Tabata et al, 1993;Yoshida and Ohshima, 1996). This accumulation of the protein in the Golgi apparatus was not described in the recent in vivo studies.…”

Section: Discussionmentioning

confidence: 81%

“…The nomenclature for the differentiation of odontoblastic lineage cells was based on previous reports (Dahl, 1983;Bishop and Boyde, 1986;Ohshima and Yoshida, 1992;Tabata et al, 1993;Sakaki and Garant, 1996;Yoshida and Ohshima, 1996), and classified as mesenchymal cells, pre-odontoblasts, columnar odontoblasts, high columnar odontoblasts or postodontoblasts ( Figure 1).…”

Section: Methodsmentioning

confidence: 99%

PTHrP and PTH/PTHrP Receptor 1 Expression in Odontogenic Cells of Normal and HHM Model Rat Incisors

Kato¹,

Suzuki²,

Karasawa³

et al. 2005

Toxicol Pathol

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Parathyroid hormone related peptide (PTHrP) was discovered as a causative factor of humoral hypercalcemia of malignancy (HHM). We examined PTHrP and its receptor (PTHR1) expression patterns in odontogenic cells in normal and HHM model rat incisors. Nontreated nude rats serving as the normal control and HHM model rats produced by implantation of PTHrP-expressing tumor (LC-6) cells were prepared. HHM rats fractured its incisor, and histopathologically, restrict population of odontoblasts showed findings classified as "shortening of high columnar odontoblasts" and "dentin niche." The incisors were immunostained against PTHrP and PTHR1. In normal rats, PTHrP and PTHR1 colocalized in ameloblasts, cementoblasts, and odontoblastic cells from mesenchymal cells to columnar odontoblasts. In high columnar odontoblasts, PTHrP solely expressed. In the HHM animals, although the expression patterns were identical to those of the normal rats in normal area, the shortened high columnar odontoblasts maintained PTHR1 expression and dentin niche comprising odontoblastic cells expressed both proteins. In the HHM model, the protein expression patterns changed in the odontoblastic cells with histological anomalies, and thus direct relations between the anomalies and PTHrP/PTHR1 axis are suggested.

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Distribution and organization of peripheral capillaries in dental pulp and their relationship to odontoblasts

Cited by 51 publications

References 47 publications

Expression patterns of nestin and dentin sialoprotein during dentinogenesis in mice

Expression patterns of nestin and dentin sialoprotein during dentinogenesis in mice

Contribution of mesoderm to the developing dental papilla

PTHrP and PTH/PTHrP Receptor 1 Expression in Odontogenic Cells of Normal and HHM Model Rat Incisors

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