The occurrence of three species of the genus Oscheius Andrássy, 1976 (Nematoda: Rhabditida) in Iran

Abstract

Due to importance and effectiveness of some entomopathogenic or insect parasitic nematodes in controlling of agricultural pests, or pests of non-agricultural plants, a study was conducted in order to identify the species of this group of nematodes in city of Tehran. As the result, three species belonging to the genus Oscheius were recovered in association with bark samples having the bark beetle galleries. Morphological and molecular data were provided for two recently recovered species of the genus, namely O. necromenus and O. onirici. Molecular data were also provided for a recently recovered isolate of O. tipulae. All three species were recovered in association with bark samples collected from dead trees in the city of Tehran. Morphological characters and morphometric data of the two aforementioned species are in accordance with the data given in their original descriptions. One recovered individual from a small bark sample characterized by its short body length was sequenced for its 28S and internal transcribed spacer (ITS) rDNA loci, and the results of BLAST search using the newly obtained partial sequences revealed that it belonged to O. tipulae. Molecular phylogenetic studies revealed recently sequenced Iranian populations of O. onirici and O. tipulae forming a clade with other isolates/populations of these species in ITS tree with maximal Bayesian posterior probability (BPP), and presently sequenced isolates of O. tipulae and O. necromenus form a clade with other isolates of these species in 28S tree. The two species O. onirici and O. necromenus were reported in Iran for the first time.

Morphological, Morphometrical and Molecular Characterization of Oscheius siddiqii Tabassum and Shahina, 2010 (Rhabditida, Rhabditidae) from India with Its Taxonomic Consequences for the Subgenus Oscheius Andrássy, 1976

“…Morphometrically, the also show close similitude to each other, such as in the size of the females; similar ratios (a, b, c, and c’); similar anal body width; similar SW%, GS% and V%; and other morphometric measurements ( Supplementary Tables S2 and S3 ). Therefore, O. rugaoensis is proposed as a junior synonym of O. chongmingensis , while the population from Japan [ 50 ] attributed to O. rugaoensis should be considered conspecific with the population of O. necromenus that is described from Iran [ 51 ].…”

First record of Oscheius myriophilus (Poinar, 1986) (Rhabditida: Rhabditidae) from Iran; and its efficacy against two economic forest trees pests, Cydalima perspectalis (Walker, 1859) (Lepidoptera: Crambidae) and Hyphantria cunea (Drury, 1773) (Lepidoptera: Erebidae) in laboratory condition

“…Oscheuis rugaoensis was reported from Iran, isolated from the white grub larvae Polyphylla adspersa Motschulsky, 1853 (Coleopera: Scarabaeidae) ( Darsouei et al, 2014 ). Valizadeh et al (2017) recovered three species O. necromenus ( Sudhaus and Schulte, 1989 ), O. onirici Torrini et al, 2015 and O. tipulae in association with bark beetle galleries in Tehran, Iran. The application of native EPNs is preferred for more successful and environmental low-risk purposes ( Labaude and Griffin, 2018 ).…”

“…Hassani-Kakhki et al (2012) reported O. tipulae (Lam and Webster, 1971) from Iran in the shape of an abstract. Oscheuis rugaoensis was reported from Iran, isolated from the white grub larvae Polyphylla ads persa (Motschulsky, 1853) (Coleopera: Scarabaeidae) (Darsouei et al, 2014). Valizadeh et al (2017 recovered three species O. necromenus (Sudhaus and Schulte, 1989), O. onirici (Torrini et al, 2015) and O. tipulae in association with bark beetle galleries in Tehran, Iran.…”

Description of Oscheius indicus n. sp. (Rhabditidae: Nematoda) from India

“…Another species, Heterorhabditidoides rugaoensis, was also transferred to Oscheius (Darsouei et al, 2014;Tabassum et al, 2016). Synonymizing Heterorhabditoides with Oscheius has been widely accepted in the field (Liu et al, 2012;Campos-Herrera et al, 2015;Torrini et al, 2015;Tabassum et al, 2016;Valizadeh et al, 2017). A total of 44 species of Oscheius have been described till date, out of which 16 are from the Indian sub-continent.…”