The occurrence of Listeria species in milk and dairy products: a national survey in England and Wales

Greenwood, M.; Roberts, Diane; Burden, Patricia

doi:10.1016/0168-1605(91)90070-6

Cited by 107 publications

(62 citation statements)

References 14 publications

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“…The rate of Listeria spp.-contamination of raw milk from farm bulk tanks in Nagano Prefecture was low (3.1%) as compared with those in other countries [4,5,9,10,13,17]. No direct comparison is possible, however, because Fenlon and Wilson [5] also showed that the differences in the incidence of the organism in raw milk was influenced by the method of isolation and sample volume.…”

Section: Discussionmentioning

confidence: 96%

Prevalence of Listeria Species in Raw Milk from Farm Bulk Tanks in Nagano Prefecture.

Yoshida

Sato

Hirai

1998

The Journal of Veterinary Medical Science

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ABSTRACT. Raw milk samples from bulk tanks of a total of 943 farms, which corresponded to approximately 60% of all dairy farms in Nagano Prefecture were examined for Listeria species between December 1990 and April 1991. Listeria spp. were isolated from 29 (3.1%) of 943 milk specimens. In the southern, central, eastern and northern areas of the prefecture, Listeria spp. were isolated from 6.1% (22/362), 1.5% (4/272), 1.4% (2/143) and 0.6% (1/166) of samples, respectively. Listeria monocytogenes was isolated from three (0.3%) bulk tanks in the southern area: two of the strains isolated from two different farm bulk tanks were serovar 4b, and the other one was 1/2a. Besides, between February 1991 and January 1992, 504 samples of raw milk from farm bulk tanks were collected nine times from 56 farms in the southern area, where the prevalence of Listeria spp. was the highest, and examined for the seasonal variation in the presence of Listeria spp. The prevalence of Listeria spp. was higher in spring (14.3%) than in autumn (4.8%). The 56 farms were divided into three groups according to the prevalence of Listeria spp., namely, three farms in Group 1 gave a high contamination rate (50%≤), 14 farms in Group 2 a low contamination rate and the remaining 39 farms in Group 3 no recovering of Listeria spp. Sixteen strains of L. monocytogenes serovar 4b were isolated from four farms. -KEY WORDS: Listeria monocytogenes, Listeria species, raw milk, seasonal variation.J. Vet. Med. Sci. 60(3): 311-314, 1998 samples were supplied by the Milk Testing Association in Nagano Prefecture. Five-milliliter samples of raw milk were collected and transported to our laboratory within 2 hr. Procedure for isolation and identification of Listeria:The 3-ml samples of milk were enriched by adding 30-ml of Listeria Enrichment Broth (Difco) and incubating at 30°C for 48 hr. After enrichment, 0.1 ml of each culture was streaked on PALCAM agar (Merck) plate, which was incubated at 37°C for 24 to 48 hr. Typical listeria-like colonies having developed on PALCAM agar plates were selected. Suspicious colonies were subcultured on TryptoSoya Agar (Nissui) supplemented with 0.5% yeast extract for characterization and purification. The plates were incubated at 37°C for 24 hr and the colonies were then reexamined in oblique transmitted light for a blue color [11]. One presumed listerial isolate per plate was biochemically identified and serologically confirmed as described by Seeliger and Höhne [16]. RESULTSThe prevalence of Listeria spp. is shown in Table 1. Listeria spp. were isolated from 29 (3.1%) samples. L. monocytogenes was detected in three (0.3%) out of 943 samples: one strain was serovar 1/2a and the other two serovar 4b. The others (2.8%) were L. innocua. The prevalence in four different sampling areas is also shown in Table 1. The highest incidence (6.1%) of Listeria spp. was found in the southern area, and the lowest incidence (0.6%) in the northern area. All three strains of L. monocytogenes were isolated from samples in the southern area.

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Section: Discussionmentioning

confidence: 96%

Prevalence of Listeria Species in Raw Milk from Farm Bulk Tanks in Nagano Prefecture.

Yoshida

Sato

Hirai

1998

The Journal of Veterinary Medical Science

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“…The milk produced on the study farm is pasteurized before its distribution to consumers, and the presence of Listeria in bulk tank milk is therefore unlikely to pose a human health hazard. Pasteurized milk from retail stores in the United States (11) and in England and Wales (14), however, tested positive for L. monocytogenes in 0.018% and 1.1% of samples, respectively. Outbreaks of human listeriosis have been attributed to the consumption of pasteurized milk (7,10) or dairy products manufactured with improperly pasteurized milk (22).…”

Section: Discussionmentioning

confidence: 99%

Molecular Ecology of Listeria monocytogenes : Evidence for a Reservoir in Milking Equipment on a Dairy Farm

Latorre

Kessel²,

Karns³

et al. 2009

Appl Environ Microbiol

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“…Goat curd has a high percentage of essential amino-acids, can lower intestinal pH value and regulate intestinal microbiota, so it's often recommended by gastroenterologists in liver disease [19]. Nevertheless, it can also be perfect medium for zoonotic bacteria transmission, such as Campylobacter, Listeria or E. coli [20]. That must be kept in mind especially if we consider the fact that presence of L. monocytogenes in raw goat milk can be up to 2.2% [20].…”

Section: Discussionmentioning

confidence: 99%

Unusual Listeria monocytogenes peritonitis in peritoneal dialysis patient with liver cirrhosis: a case report and review of literature

et al. 2017

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The occurrence of Listeria species in milk and dairy products: a national survey in England and Wales

Cited by 107 publications

References 14 publications

Prevalence of Listeria Species in Raw Milk from Farm Bulk Tanks in Nagano Prefecture.

Prevalence of Listeria Species in Raw Milk from Farm Bulk Tanks in Nagano Prefecture.

Molecular Ecology of Listeria monocytogenes : Evidence for a Reservoir in Milking Equipment on a Dairy Farm

Unusual Listeria monocytogenes peritonitis in peritoneal dialysis patient with liver cirrhosis: a case report and review of literature

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