The Occurrence ofbla_CTX-M,bla_SHV, andbla_TEMGenes in Extended-Spectrumβ-Lactamase-Positive Strains ofKlebsiella pneumoniae,Escherichia coli, andProteus mirabilisin Poland

Abstract

Bacteria belonging to theEnterobacteriaceaefamily that produce extended-spectrumβ-lactamase (ESBL) enzymes are important pathogens of infections. Increasing numbers of ESBL-producing bacterial strains exhibiting multidrug resistance have been observed. The aim of the study was to evaluate the prevalence ofblaCTX-M,blaSHV, andblaTEMgenes among ESBL-producingKlebsiella pneumoniae,Escherichia coli, andProteus mirabilisstrains and to examine susceptibility to antibiotics of tested strains. In our study, thirty-six of the tested strains exhibitedblaCTX-Mgenes(blaCTX-M-15,blaCTX-M-3,blaCTX-M-91, andblaCTX-M-89). Moreover, twelve ESBL-positive strains harboredblaSHVgenes(blaSHV-18,blaSHV-7,blaSHV-2, andblaSHV-5), and the presence of ablaTEMgene(blaTEM-1)in twenty-five ESBL-positive strains was revealed. AmongK. pneumoniaethe multiple ESBL genotype composed ofblaCTX-M-15, blaCTX-M-3, blaSHV-18, blaSHV-7, blaSHV-2, andblaSHV-5genes encoding particular ESBL variants was observed. Analysis of bacterial susceptibility to antibiotics revealed that, amongβ-lactam antibiotics, the most effective againstE. colistrains was meropenem (100%), whereasK. pneumoniaewere completely susceptible to ertapenem and meropenem (100%), andP. mirabilisstrains were susceptible to ertapenem (91.7%). Moreover, among non-β-lactam antibiotics, gentamicin showed the highest activity toE. coli(91.7%) and ciprofloxacin the highest toK. pneumoniae(83.3%).P. mirabilisrevealed the highest susceptibility to amikacin (66.7%).

Antimicrobial resistance in extended spectrum -lactamases (ESBL)-producing Escherichia coli isolated from human urinary tract infections in Ndjamena, Chad

“…It was shown that resistant genes for β-lactams are often located in mobile genetic elements such as plasmids and integrons, whereby the horizontal transfer of these genes is possible not only in bacteria of the same species but also between bacteria of different species (Bush et al, 2008;Dominika et al, 2014). This characteristic location of genes responsible for resistance could explain the high prevalence of ESBL-producers among strains of E. coli observed in this work Interestingly, our data indicated that infection with ESBL-producing E. coli was significantly higher in adults than in children.…”

Detection of virulence and antimicrobial resistance genes in Escherichia coli isolates from diarrhoiec dogs in Iran

“…In E. coli, the CITM gene cluster encodes AmpC β-lactamase which hydrolyses β-lactams (Van et al 2008). Detection of bla(SHV) in 6 (42.9%) of the examined isolates, suggested high prevalence of this SHV β-lactamase-encoding gene (Feria et al 2002, Ojdana et al 2014. The bla(SHV) gene encodes β-lactamase which mediates resistance to cephalothin, a first-generation cephalosporin.…”

“…The bla(SHV) gene encodes β-lactamase which mediates resistance to cephalothin, a first-generation cephalosporin. However, some variants of bla(SHV) encode extended-spectrum β-lactamase which hydrolyses third-generation cephalosporins (extended-spectrum β-lactams) (Bradford 2001, Bush and Jacoby 2010, Ojdana et al 2014, these variants have been reported in faecal E. coli isolates from dogs (Rocha-Gracia et al 2015, Schmidt et al 2015). Therefore, the 42.9% bla(SHV) detection rate in this study suggested that many dogs with diarrhoea in Iran may harbor extended-spectrum β-lactam (ESBL)-resistant E. coli.…”

First Report of Extended-Spectrum β-Lactamases Among Clinical Isolates ofKlebsiella pneumoniaein Gaza Strip, Palestine

“…K. pneumoniae isolates carrying multiple b-lactamase genes have been previously reported in other studies. 13,[43][44][45] Four isolates coexpressed bla CTX-M-15 along with other ESBLs. Three isolates harbored both SHV-5 and CTX-M-15, and only one isolate coproduced both SHV-12 and CTX-M-15.…”