The occurrence of Helicobacter pylori antigens in dental plaque; an association with oral health status and oral hygiene practices

Namiot, DB; Leszczyńska, Katarzyna; Namiot, Z; Chilewicz, M; Bucki, Robert; Kemonä, A

doi:10.2478/v10039-010-0032-5

Cited by 27 publications

(24 citation statements)

References 15 publications

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“…Most published studies have aimed to investigate the oral cavity as a source for subsequent gastric infections, which, of course, is an important issue in this field of research (Namiot et al, 2010;Chaudhry et al, 2011;Silva et al, 2010;de Souza Gonçalves et al, 2009;Bago et al, 2011;Eskandari et al, 2010). In contrast to the results presented here, many studies clearly reported the occurrence of H. pylori in dental plaque and concluded that there was an association between gastroesophageal disease and dental oral status (Silva et al, 2009(Silva et al, , 2010; Morales-Espinosa et al, …”

Section: Discussionmentioning

confidence: 59%

“…The first contact between humans and H. pylori, leading to infection, takes place in the oral cavity. However, in the literature, it is evident that H. pylori is considered a typical member of the oral flora (Aas et al, 2005;Baumgartner et al, 2009;Namiot et al, 2010;Rickard et al, 2003). On the other hand, no cultivation of H. pylori has been reported from the oral cavity to date (AlAhmad et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

“…If PCR was not used, some authors concluded the occurrence of H. pylori in the oral cavity after detection of some antigens such as those revealed by the kit used for detection of antigens in stool samples (Namiot et al, 2010). Moreover, on the basis of PCR results, some authors have suggested that saliva and dental plaque may serve as a temporary reservoir for H. pylori in individuals with gastric disease (Silva et al, 2009).…”

Section: ; De Souza Gonçalves Et Al 2009)mentioning

confidence: 99%

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Is Helicobacter pylori resident or transient in the human oral cavity?

Al‐Ahmad

Kürschner

Weckesser

et al. 2012

Journal of Medical Microbiology

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Helicobacter pylori colonizes the stomachs of at least half of the world's human population. The role of the oral cavity in this colonization is not clear and there are, to date, no comprehensive data that clearly demonstrate the isolation of this bacterium from the oral cavity. The aim of this study was to evaluate the prevalence of H. pylori in the oral cavity of 15 patients who tested positive for H. pylori. A comprehensive dental examination of all patients was conducted. Samples were taken from supragingival and subgingival plaque, saliva, periapical exudates and tongue swabs. All samples were taken before the application of antibiotics. A total of 163 oral samples were investigated by PCR using two different H. pylori-specific primer pairs. A PCR inhibition control using a modified plasmid was always included for the most specific primer pair. In addition, a culture technique was used to confirm PCR results. Despite a PCR detection limit of 10 2 bacteria ml "1, out of 14 patients, H. pylori could not be detected in any of the samples taken. In one patient, H. pylori-positive PCR signals were obtained in two samples using only one primer pair. H. pylori could not be cultivated from these two PCR-positive samples; therefore, no correlation to oral colonization status could be established. This study challenges the misleading preconception that H. pylori resides in the human oral cavity and suggests that this bacterium should be considered transient and independent of the oral status. To date, positive PCR results for H. pylori in the oral cavity have been overestimated and not critically interpreted in literature.

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Section: Discussionmentioning

confidence: 59%

Section: Discussionmentioning

confidence: 99%

Section: ; De Souza Gonçalves Et Al 2009)mentioning

confidence: 99%

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Is Helicobacter pylori resident or transient in the human oral cavity?

Al‐Ahmad

Kürschner

Weckesser

et al. 2012

Journal of Medical Microbiology

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“…The diagnostic criteria followed the WHO protocol [34]. Oral examination involved assessment of dentition (number of teeth, carious teeth, plaque index and gingival index), halitosis and hygienic procedures (frequency of daily toothbrushing, sort of used toothbrush, a yearly frequency of changing toothbrush, feeding with premasticated food, common usage of glasses, spoons in a family) [25, 35–42]. …”

Section: Methodsmentioning

confidence: 99%

The investigation of Helicobacter pylori in the dental biofilm and saliva samples of children with dyspeptic complaints

et al. 2017

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BackgroundThe oral cavity can be an extra-gastric reservoir for Helicobacter pylori (H.pylori). This can play a role in the pathogenesis of halitosis, glossitis, recurrent aphthous stomatitis, and dental caries. The present study was conducted to detect the presence of H.pylori within the dental biofilm and in saliva samples collected from children suffering from dyspepsia and children without any gastrointestinal complaints. Associations with gastric infection, halitosis, and some oral parameters were also evaluated.MethodsSeventy children (aged between 5–16) with dyspepsia were selected for the study group and control group composed of 30 healthy children without dyspepsia were also included in the study. After detailed oral and clinical examinations for oral parameters, saliva, and supragingival dental biofilm samples were collected for 16S rRNA and 23S rRNA genes detection by real-time polymerase chain reaction (RT-PCR). The presence of gastric H.pylori was evaluated in endoscopic biopsy specimens histopathologically. Halitosis was evaluated by benzoyl-DL-arginine-naphthylamid (BANA) test. Salivary S.mutans and Lactobacilli sp. counts were also carried out by commercial kits.Results H.pylori was histopathologically detected amongst 83% of the children with the dyspeptic condition. The detection rate of this bacteria in dental biofilm and saliva samples and halitosis were found relatively higher in the dyspeptic children rather than the control group (p < 0.01). Halitosis was not significantly different between dyspeptic children and those detected with H.pylori (p > 0.05). In the gastric H.pylori positive group with dyspepsia, DMFT/S and dmft/s numbers and plaque indices were found higher than the control group (p < 0.01). Only plaque indices of gastric H.pylori negative group with dyspepsia were found higher than the control group (p < 0.01). S.mutans and Lactobacilli sp. counts were not significantly different between gastric H.pylori positive and negative groups (p > 0.05). Comparing to those with negative for both genes, in children whose dental biofilm and saliva samples were positive for both 16S rRNA and 23S rRNA genes, significantly higher results for halitosis, and DMFS numbers and significantly lower results for dmfs numbers and pH values were found (p < 0.01).Conclusions Helicobacter pylori can occur in the oral cavity aside and independently from the stomach. However, the high number of bacteria in the oral cavities of children with gastric H.pylori, an association between the presence of H.pylori and halitosis, DMFS, and pH were found.Electronic supplementary materialThe online version of this article (doi:10.1186/s12903-017-0361-x) contains supplementary material, which is available to authorized users.

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“…A search of Scopus detected 221 articles that have ‘Helicobacter pylori' and ‘oral' in the title, 7 titles that also include ‘antigen' in the title, and only 1 title that is about detection of oral H. pylori antigen in humans [17]. Namiot et al [17] screened 155 patients who had no history of H. pylori infection and found that 65.6% were positive for H. pylori in dental plaque using the Oxoid IDEA Hp StAR amplified immunoassay (the test uses monoclonal antibodies to detect fecal antigen). It has been proposed that salivary H. pylori originates from gastrointestinal reflux [18].…”

Section: Discussionmentioning

confidence: 99%

A Screening Trial of <b><i>Helicobacter pylori</i></b>-Specific Antigen Tests in Saliva to Identify an Oral Infection

Yee¹,

Wei²,

Yee³

et al. 2013

Digestion

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Objective:Helicobacter pylori infection places a heavy burden on medical and economic resources. Standard diagnosis requires the presence of established H. pylori gastric disease. Study Design and Setting: A multicenter screening trial assessing 2 immunochromatographic H. pylori antigen oral tests was carried out with 201 participants. The analysis also included a urea breath test (UBT), a Campylobacter-like organism test, silver stain, culture, serology, and stool tests. Results: The participants were grouped into UBT positive (UBT+) and UBT negative (UBT-) people, using conventional methods with congruent clusters based on p values from McNemar's paired χ² analysis and 95% CI estimates. Both oral tests were also positive in 82% of the seropositive UBT- people. However, oral antigen and seroprevalence divided UBT- people into 2 statistically separate CI subgroups: the UBT- symptomatic (highly positive) group and the UBT- asymptomatic (mostly negative) group. 90.5% of all people whose oral tests were both negative were also UBT-. Conclusions: Saliva H. pylori antigen is an important indicator in UBT- asymptomatic patients. Currently, its clinical significance remains uncertain, but saliva may be a reservoir from where H. pylori is transmitted to the stomach. In symptomatic patients, it is strongly associated with stomach infection.

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The occurrence of Helicobacter pylori antigens in dental plaque; an association with oral health status and oral hygiene practices

Cited by 27 publications

References 15 publications

Is Helicobacter pylori resident or transient in the human oral cavity?

Is Helicobacter pylori resident or transient in the human oral cavity?

The investigation of Helicobacter pylori in the dental biofilm and saliva samples of children with dyspeptic complaints

A Screening Trial of <b><i>Helicobacter pylori</i></b>-Specific Antigen Tests in Saliva to Identify an Oral Infection

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