The Occurrence of Bioactive Micromonosporae in Aquatic Habitats of the Sunshine Coast in Australia

Abstract

Abstract:Screening strategies based on the ecological knowledge of antibiotic producing microorganisms and their roles in the natural environment are being increasingly employed in the search for novel antibiotic agents. Micromonosporae are common inhabitants of aquatic habitats and have proved to be a continuing source of novel bioactive compounds including antibacterial and antitumor agents. The ecological distribution and frequency of bioactive micromonosporae in Sunshine Coast region aquatic habitats were studied through a range of selective isolation procedures designed to negatively select against the isolation of unwanted microbial taxa commonly associated with marine environments. It was revealed that bioactive compound producing species of micromonosporae were present in the aquatic habitats of the Sunshine Coast region in Australia.

Isolation and characterization of potential antibiotic producing actinomycetes from water and sediments of Lake Tana, Ethiopia

“…To obtain even growth, the entire Mueller Hinton agar (MHA) surface was swabbed uniformLy by the cotton swab. The inoculated plates were left at room temperature for [3][4][5] minutes to allow for any surface moisture to be absorbed before applying the extract. Ten mg/mL of each crude extracts was impregnated with filter paper (6 mm diameter disc) and placed on the surface of MHA medium.…”

“…The test tubes were incubated at 37 °C for 24 h until the visible turbidity and density equal to that of 0. 5 McFarland standard. After adjusting the turbidity, sterile cotton swab was dipped into the suspension and streaked over the entire surface of the plate medium for three times by rotating the plate approximately at 60 °C to ensure uniform distribution of the inoculum.…”

“…In perpendicular streak method, nutrient agar media was used and each plate was streaked with individual isolates at the center/diameter of the plate and incubated at 30 °C for 7 d. Later, 24 h fresh sub-cultured test bacteria were prepared and streaked perpendicular to the isolates and incubated at 37 °C for 24 h [30] . In agar overlay method, [5][6][7] d old colony of actinomycetes was spot inoculated on nutrient agar plates and incubated for 7 d at 30 °C . Then overlaid with 5 mL of semisolid nutrient agar with standardized suspension of test bacteria was inoculated and incubated at 37 °C for 24 h. The zone of inhibition (in mm) around the colonies was measured.…”

“…They are found in freshwater and marine water habitats [1][2][3] . The dominant actinomycetes Micromonospora can be isolated from aquatic habitats such as streams, rivers, lake mud, river sediments, beach sands, sponge and marine sediments [4,5] . Several novel bioactive compounds were discovered from aquatic actinomycetes, for example rifamycin from Micromonospora [6] ; salinosporamide-A, an anticancer metabolite from a Salinispora strain [7] ; marinomycins from Marinophilus sp.…”

Production, Structural Elucidation and Biological Activities of Bioactive Metabolite of MarineSaccharomonospora viridi

“…The search for novel therapeutic agents for use in the pharmaceutical industry is driven by the need to combat the increase in the incidence of infection due to antibiotic resistant pathogens coupled with the search for antitumor and antiviral compounds (Eccleston et al, 2008). The decreasing rate of discovery of novel drugs from established terrestrial sources has motivated the evaluation of new sources of chemically diverse bioactive compounds (Magarvey et al, 2004).The increasing number of novel metabolites from marine microorganisms reported manually shows that marine habitats are a promising source of biotechnological commercially significant products (Blunt et al, 2007).…”