Actinobacteria in Marine Environments

Kurtböke, D. İpek; Okazaki, Takao; Vobis, Gernot

doi:10.1002/9781119143802.ch86

Cited by 2 publications

(1 citation statement)

References 208 publications

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“…Marine habitats and especially the intertidal zones are occupied by diverse organisms. In order to survive in these extreme habitats, bacteria need to develop unique strategies of survival through the synthesis of novel metabolites, proteins and inhibitors 20 . Current study focused on identi cation and characterization of extracellular PIs from Streptomyces species inhabiting the intertidal zones.…”

Section: Discussionmentioning

confidence: 99%

Diversity and plasticity of simultaneously expressing extracellular protease inhibitors from four marine Streptomyces spp. isolated from West coast of India

Shintre

Deshpande

Patwardhan

et al. 2022

Preprint

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Streptomyces have long been studied for their defense metabolites and Protease Inhibitors (PIs) they secrete for mediating biotic interactions. Current study focuses on diversity of extracellular PIs obtained from four marine Streptomyces species isolated from intertidal regions of West Coast of India. Streptomyces PIs showed strong inhibition of subtilisin followed by trypsin and chymotrypsin. Using shotgun proteomics approach, we identified 15 Streptomyces PIs belonging to 4 PI families namely Streptomyces subtilisin inhibitor (SSI), Potato peptidase inhibitor, Ovomucoid and Serpin. This is a first report of identification of Ovomucoid and Potato peptidase inhibitor families from Streptomyces. Amongst the 15 PIs, 12 were SSIs with 20-75% sequence similarity and variations in the conserved 73rd aa residue. Moreover, 10 SSI isoforms were co-expressed in a single species S. longispororuber. In-silico and in-vitro assays with proteases from microbial, bovine and insect sources suggested that the Streptomyces PIs had a broad-spectrum inhibitory activity. In co-culture of Streptomyces with other protease producing bacteria, cellular secretions differentially affected growth, sporulation and/or pigment production. Present study elucidates the diversity and plasticity of extracellular PIs from marine Streptomyces spp. Further, their role in chemical ecology of the bacterial communities as well as their potential applications in therapeutics are discussed.

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Section: Discussionmentioning

confidence: 99%

Diversity and plasticity of simultaneously expressing extracellular protease inhibitors from four marine Streptomyces spp. isolated from West coast of India

Shintre

Deshpande

Patwardhan

et al. 2022

Preprint

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Effects of Fresh Groundwater and Seawater Mixing Proportions and Salt-Freshwater Displacement on Coastal Aquifer Microbial Communities

Chen,

Ma,

et al. 2024

Water

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Seawater intrusion significantly affects the microbial communities within coastal aquifers. Investigating the spatial distribution of groundwater microbial communities in coastal regions is crucial for understanding seawater intrusion. The primary objective of this study is to develop a novel microbial index-based method for detecting seawater intrusion. Groundwater microbial samples were collected and sent to the laboratory in the west coastal area of Longkou City, Shandong Province. By characterizing the microbial community within the whole interval of seawater intrusion into fresh groundwater and discussing the effects of salt-freshwater displacement intensities on groundwater microbial communities, including diversity, structure, and function, using indoor domestication experiments, we reveal the response of microorganisms to the seawater intrusion process under in situ environmental conditions. The results show that the microbial community diversity is highest in environments with a seawater mixing proportion (P(sm)) of 2.5% and lowest in those with a P(sm) of 75%. When considering species abundance and evolutionary processes, the microbial community structure is similar at higher P(sm) levels, while it is similar at lower P(sm) levels based on the presence or absence of species. Tenericutes, Flavobacteriia, Rhodobacterales, Flavobacteriales, Rhodobacteraceae, Flavobacteriaceae, Cohaesibacteraceae, and Cohaesibacter are significantly positively correlated with the P(sm). Strong salt-freshwater displacement enhanced the richness and evenness of the microbial community, whereas weak displacement showed the opposite trend. Strong displacement affects the functional profiles of the microbial community. This study effectively addressed the challenge of obtaining samples in coastal areas and also incorporated salt-freshwater displacement intensities, which can more comprehensively describe the microbial community characteristics within the groundwater of coastal aquifers.

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Actinobacteria in Marine Environments

Cited by 2 publications

References 208 publications

Diversity and plasticity of simultaneously expressing extracellular protease inhibitors from four marine Streptomyces spp. isolated from West coast of India

Diversity and plasticity of simultaneously expressing extracellular protease inhibitors from four marine Streptomyces spp. isolated from West coast of India

Effects of Fresh Groundwater and Seawater Mixing Proportions and Salt-Freshwater Displacement on Coastal Aquifer Microbial Communities

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