The occurrence of atypical enteropathogenic Escherichia coli strains among children with diarrhea in Iran

Bakhshi, Bita; Fallahzad, Soheil; Pourshafie, Mohammad Reza

doi:10.1007/s10156-012-0526-0

Cited by 19 publications

(14 citation statements)

References 21 publications

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“…The gene cassettes ranged between 750 base pairs and 2000 base pairs, and amplicons of 1500 base pairs and 2000 base pairs were the most common gene cassette regions harbored in class 1 and 2 integrons, respectively. Moreover Machado et al 2007 [32] and Bakhshi et al 2012 [33] published similar results confirming our investigation.…”

Section: Discussionsupporting

confidence: 91%

High Prevalence of Class 1 to 3 Integrons Among Multidrug-Resistant Diarrheagenic Escherichia coli in Southwest of Iran

Kargar

Mohammadalipour

Doosti

et al. 2014

Osong Public Health and Research Perspectives

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ObjectivesHorizontal transfer of integrons is one of the important factors that can contribute to the occurrence of multidrug-resistant (MDR) bacteria. This study aimed to determine the prevalence of integrons among MDR Escherichia coli strains isolated from stool specimens and investigate the associations between the existence of integrons and MDR properties in the southwest of Iran.MethodsThere were 164 E. coli strains isolated from January 2012 to June 2012. Fecal specimens identified as E. coli by the conventional methods. Subsequently the antibiotic resistance was assessed using Clinical and Laboratory Standard Institute criteria. The presence of class 1–3 integrons and embedded gene cassettes was verified using specific primers by multiplex polymerase chain reaction assay.ResultsAmong a total of 164 studied samples, 69 (42.07%) isolates were multidrug resistant. Class 1 and class 2 integrons were present in 78.26% and 76.81% MDR isolates, respectively. For the first time in Iran, class 3 integron was observed in 26.09% MDR isolates. Significant correlations were identified between: class 1 integron and resistance to amikacin, gentamicin, chloramphenicol, ampicillin, tetracycline, nalidixic acid, and co-trimoxazole; class 2 integron and resistance to aminoglycosides, co-trimoxazole, cefalexin, ampicillin, and chloramphenicol; and class 3 integron and resistance to gentamicin, kanamycin, and streptomycin.ConclusionOur results indicate that integrons are common among MDR isolates and they can be used as a marker for the identification of MDR isolates. Therefore, due to the possibility of a widespread outbreak of MDR isolates, molecular surveillance and sequencing of the integrons in other parts of the country is recommended.

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Section: Discussionsupporting

confidence: 91%

High Prevalence of Class 1 to 3 Integrons Among Multidrug-Resistant Diarrheagenic Escherichia coli in Southwest of Iran

Kargar

Mohammadalipour

Doosti

et al. 2014

Osong Public Health and Research Perspectives

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“…Disease symptoms may begin from mild diarrhea to severe bloody diarrhea [hemorrhagic colitis (HC)] especially in children (1-4 years) [24]. Other reports found that EPEC are associated with outbreaks of infantile diarrhea among children in developing countries [25]. These previous causes paid our attention to make this study.…”

Section: Discussionmentioning

confidence: 97%

Characterization of verotoxigenic E.coli and enteropathogenic E.coli isolated from infants with diarrhea in combination with antimicrobial resistance pattern in Minia, Egypt

Gany

Gad

Mousa

et al. 2020

Journal of advanced Biomedical and Pharmaceutical Sciences

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Diarrheal disease is considered a significant health problem, principally in developing countries as it is one of the leading causes of morbidity and mortality especially in infants. Studies found that STES and EPEC strains are among the most predominant causative agents in acute diarrhea. The aim of the study was to investigate the presence of certain virulence factors and antibiotic resistances of STEC and EPEC originating from infants with diarrhea in Egypt. A total of 200 infants of different ages suffering from diarrhea between August 2017 and February 2019 were registered in this study. Standard cultural and biochemical methods were used to isolate E. coli species while; PCR was applied for the detection of virulence genes (stx1, stx2, eae and pfb A) for STEC and EPEC isolates, respectively. Eighty nine (44.5 %) E. coli strains were isolated from 200 stool samples. A total of 40 (44.9 %) strains were identified as STEC, and 1(1.12 %) strain was atypical EPEC. Fourteen of the STEC serotypes were O157:H7. Susceptibilities of 40 STEC and 1 aEPEC isolates were determined for ten antimicrobial drugs. STEC O157:H7 strains and STEC Non-O157:H7 exhibited the highest resistance against cefazolin and no resistance against imipnem and Amikacin, respectively while, the isolated strain of aEPEC was sensitive to all the studied antibiotics except cefazolin. Results showed that STEC is one of the major causes of diarrhea that showed resistant to commonly used antibiotics so representatives must pay great attention to this matter in order to increase the health of the community.

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“…Likewise, 92% of EPEC isolates collected from children in Brazil between 2001 and 2002 were atypical (66), compared to 38% in a 1998-1999 study (52). More recently, 39.3% of EPEC strains isolated from children with diarrhea in Iran tested positive for the bfp gene, while the remaining 61.7% appeared as aEPEC, which lacks bfp (67). Studies from Norway (68) and Australia (69) also suggest that aEPEC isolates are more commonly found among persistent cases of diarrhea than typical isolates.…”

Section: Epidemiologymentioning

confidence: 99%

Recent Advances in Understanding Enteric Pathogenic Escherichia coli

et al. 2013

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SUMMARY Although Escherichia coli can be an innocuous resident of the gastrointestinal tract, it also has the pathogenic capacity to cause significant diarrheal and extraintestinal diseases. Pathogenic variants of E. coli (pathovars or pathotypes) cause much morbidity and mortality worldwide. Consequently, pathogenic E. coli is widely studied in humans, animals, food, and the environment. While there are many common features that these pathotypes employ to colonize the intestinal mucosa and cause disease, the course, onset, and complications vary significantly. Outbreaks are common in developed and developing countries, and they sometimes have fatal consequences. Many of these pathotypes are a major public health concern as they have low infectious doses and are transmitted through ubiquitous mediums, including food and water. The seriousness of pathogenic E. coli is exemplified by dedicated national and international surveillance programs that monitor and track outbreaks; unfortunately, this surveillance is often lacking in developing countries. While not all pathotypes carry the same public health profile, they all carry an enormous potential to cause disease and continue to present challenges to human health. This comprehensive review highlights recent advances in our understanding of the intestinal pathotypes of E. coli .

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The occurrence of atypical enteropathogenic Escherichia coli strains among children with diarrhea in Iran

Cited by 19 publications

References 21 publications

High Prevalence of Class 1 to 3 Integrons Among Multidrug-Resistant Diarrheagenic Escherichia coli in Southwest of Iran

High Prevalence of Class 1 to 3 Integrons Among Multidrug-Resistant Diarrheagenic Escherichia coli in Southwest of Iran

Characterization of verotoxigenic E.coli and enteropathogenic E.coli isolated from infants with diarrhea in combination with antimicrobial resistance pattern in Minia, Egypt

Recent Advances in Understanding Enteric Pathogenic Escherichia coli

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