Characterization of verotoxigenic E.coli and enteropathogenic E.coli isolated from infants with diarrhea in combination with antimicrobial resistance pattern in Minia, Egypt

Gany, Shimaa Salah Hassan Abd El; Gad, Gamal Fadl Mahmoud; Mousa, Suzan Omar; Ibrahem, Reham Ali

doi:10.21608/jabps.2020.24153.1070

Cited by 3 publications

(2 citation statements)

References 41 publications

(44 reference statements)

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“…These results differ from those obtained from Minia, Egypt [29], Tunisia [27], and the study done in Mangalore, India [30]. Mismatching of results may be due to the fact that the most common etiology of acute diarrhea in adults is viral infections which are self-limited conditions, while foodborne illness, and comorbidities that are related to bacterial causes of acute diarrhea in adults.…”

Section: Discussioncontrasting

confidence: 80%

Molecular Detection and charchterization of stx toxins of Escherichia coli O157:H7, Escherichia coli O26:H8, and Escherichia coli O111:H8 from diarrhoeic patients in Khartoum State, Sudan

Musa

Ahmed

Babikir

2023

Preprint

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Introduction/Background: Enterohaemorrhagic Escherichia coli (EHEC) O157:H7 is the most important serotype of E. coli responsible for serious intestinal and extra-intestinal disorders worldwide, but no molecular based study for EHEC as the causative agent diarrhea was performed in the Sudan. EHEC is a subset of Shiga toxin (stx) producing E. coli (STEC) which includes well recognized human pathogens. STEC produce high level toxins of a protein family referred to as Shiga toxin and Shiga-like toxins (SLTs; alternatively known as verocytotoxins VTEC or VTs). EHEC O26:H11 and O111:H8 have emerged as the most important non-O157:H7 EHEC. These toxins are mediating intestinal disease in children and adults, and if the cases left untreated these toxins may contribution to the development of extra-intestinal sequelae e.g., the haemolytic uraemic syndrome and neurological disorders. Objective: This study was conducted for the detection and molecular characterization of Shiga like toxin (stx) of E. coli O157:H7, E. coli O26:H11, and E. coli O111:H8 in Sudanese patients with diarrhea using PCR and multiplex PCR. Materials and methods: A cross sectional laboratory-based study was carried out in Khartoum, Sudan during the period from June to October 2020. All patients with confirmed E. coli diarrheal disease was included in this study. The isolates have been identified by using the biochemical, serological, molecular identification and characterization tests. SPSS IBM (software version 20.0) was used for analyses. Results: Out of 100 patients with diarrheal disease a total of 96 patients were confirmed of having E. coli organisms. All 96 E. coli bacteria were seropositive identified as members of the EHEC. Ten isolates of E. coliO157:H7, E. coli O26:H11, E. coli O111:H8 have been selected as follows; five, three, and two respectively. The selected isolates followed by multiplex PCR to detect the genes of stx toxins of E. coli O157:H7, E. coli, O26:H11, and E. coli O111:H8 and comparing between them. The multiplex PCR revealed that E. coli O157:H7 were carrying stx1 and stx2 genes represented by two bands, while E. coliO26:H11 and E. coli O111:H8 were carrying one stx2 gene represented by one band. Conclusions: E. coli O157:H7 produce stx1 and stx2 while the other serotypes E. coli O26:H11 and E. coli O111:H8 produce stx2 only. Effective control of pathogenic strains of E. coli requires a multifaceted approach. To reduce the frequency and severity of human exposure to STEC, controlling dietary sources and environmental sources such as cattle, water troughs, feed and manure is critical in breaking the cycle of infection and re-reinfecting with STEC.

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Section: Discussioncontrasting

confidence: 80%

Molecular Detection and charchterization of stx toxins of Escherichia coli O157:H7, Escherichia coli O26:H8, and Escherichia coli O111:H8 from diarrhoeic patients in Khartoum State, Sudan

Musa

Ahmed

Babikir

2023

Preprint

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“…In 2017, Hamed et al [ 35 ] isolated STEC by 23.68% from minced meat, beef burger, sausage and karish cheese. Abd El-Gany et al (2020) [ 36 ] isolated STEC in a percentage of 44.9% from stool samples of infants suffering from diarrhea, and 35% of these isolates were STEC O157. Finally, Elmonir et al (2021) [ 37 ] isolated STEC from 6, 7, 12 and 10% of collected milk, beef, diarrhetic cattle and human samples, respectively.…”

Section: Discussionmentioning

confidence: 99%

Evaluating the Phenotypic and Genomic Characterization of Some Egyptian Phages Infecting Shiga Toxin-Producing Escherichia coli O157:H7 for the Prospective Application in Food Bio-Preservation

El-Sayed

Elsayed

Amin

et al. 2022

Biology

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Shiga toxin-producing E. coli (STEC) is considered a worldwide public health and food safety problem. Despite the implementation of various different approaches to control food safety, outbreaks persist. The aim of study is to evaluate the applicability of phages, isolated against STEC O157:H7, as prospective food bio-preservatives. Considering the relatively wide host range and greatest protein diversity, two phages (STEC P2 and P4) from four were furtherly characterized. Complete genome analysis confirmed the absence of toxins and virulence factors—encoding genes. The results confirmed the close relation of STEC P2 to phages of Myoviridae, and STEC P4 to the Podoviridae family. The phages retained higher lytic competence of 90.4 and 92.68% for STEC P2 and P4, respectively with the HTST pasteurization. The strong acidic (pH 1) and alkaline (pH 13) conditions had influential effect on the surviving counts of the two phages. The lowest survivability of 63.37 and 86.36% in STEC P2 and P4 lysate, respectively appeared in 2% bile salt solution after 3 h. The results confirmed the strong effect of simulated gastric fluid (SGF) on the survivability of the two phages comparing with simulated intestinal fluid (SIF). Therefore, the two phages could be applied as a natural alternative for food preservation.

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Co-Existence of Certain ESBLs, MBLs and Plasmid Mediated Quinolone Resistance Genes among MDR E. coli Isolated from Different Clinical Specimens in Egypt

et al. 2021

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The emergence of multi-drug resistant (MDR) strains and even pan drug resistant (PDR) strains is alarming. In this study, we studied the resistance pattern of E. coli pathogens recovered from patients with different infections in different hospitals in Minia, Egypt and the co-existence of different resistance determinants. E. coli was the most prevalent among patients suffering from urinary tract infections (62%), while they were the least isolated from eye infections (10%). High prevalence of MDR isolates was found (73%) associated with high ESBLs and MBLs production (89.4% and 64.8%, respectively). blaTEM (80%) and blaNDM (43%) were the most frequent ESBL and MBL, respectively. None of the isolates harbored blaKPC and blaOXA-48 carbapenemase like genes. Also, the fluoroquinolone modifying enzyme gene aac-(6′)-Ib-cr was detected in 25.2% of the isolates. More than one gene was found in 81% of the isolates. Azithromycin was one of the most effective antibiotics against MDR E. coli pathogens. The high MAR index of the isolates and the high prevalence of resistance genes, indicates an important public health concern and high-risk communities where antibiotics are abused.

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Characterization of verotoxigenic E.coli and enteropathogenic E.coli isolated from infants with diarrhea in combination with antimicrobial resistance pattern in Minia, Egypt

Cited by 3 publications

References 41 publications

Molecular Detection and charchterization of stx toxins of Escherichia coli O157:H7, Escherichia coli O26:H8, and Escherichia coli O111:H8 from diarrhoeic patients in Khartoum State, Sudan

Molecular Detection and charchterization of stx toxins of Escherichia coli O157:H7, Escherichia coli O26:H8, and Escherichia coli O111:H8 from diarrhoeic patients in Khartoum State, Sudan

Evaluating the Phenotypic and Genomic Characterization of Some Egyptian Phages Infecting Shiga Toxin-Producing Escherichia coli O157:H7 for the Prospective Application in Food Bio-Preservation

Co-Existence of Certain ESBLs, MBLs and Plasmid Mediated Quinolone Resistance Genes among MDR E. coli Isolated from Different Clinical Specimens in Egypt

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