The occurrence and nature of electrochemical activity in milk from a herd of dairy cows

Brain, Jarod; Collier, W. A.; Hart, A. L.

doi:10.1017/s0022029905001676

Cited by 6 publications

(3 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The relative standard deviation (STD) for all measurements placed within the limit for reliable measurements (RSD < 5%) [26,27]. These results validate that the procedure is suitable for the development of a sensitive and reproducible aptasensor.…”

Section: Resultsmentioning

confidence: 66%

An Electrochemical Impedimetric Aptasensing Platform for Sensitive and Selective Detection of Small Molecules Such as Chloramphenicol

Pilehvar

Dierckx

Blust

et al. 2014

Sensors

View full text Add to dashboard Cite

We report on the aptadetection of chloramphenicol (CAP) using electrochemical impedance spectroscopy. The detection principle is based on the changes of the interfacial properties of the electrode after the interaction of the ssDNA aptamers with the target molecules. The electrode surface is partially blocked due to the formation of the aptamer-CAP complex, resulting in an increase of the interfacial electron-transfer resistance of the redox probe detected by electrochemical impedance spectroscopy or cyclic voltammetry. We observed that the ratio of polarization resistance had a linear relationship with the concentrations of CAP in the range of 1.76–127 nM, and a detection limit of 1.76 nM was obtained. The covalent binding of CAP-aptamer on the electrode surface combined with the unique properties of aptamers and impedimetric transduction leads to the development of a stable and sensitive electrochemical aptasensor for CAP.

show abstract

Section: Resultsmentioning

confidence: 66%

An Electrochemical Impedimetric Aptasensing Platform for Sensitive and Selective Detection of Small Molecules Such as Chloramphenicol

Pilehvar

Dierckx

Blust

et al. 2014

Sensors

View full text Add to dashboard Cite

show abstract

“…The response of the CDH modified SPCEs as a function of pH showed that the response current for both immobilised enzymes attained a maximum at pH 4.5 (citrate buffer containing 0.1 M KCl), that was used in the following experiments. In order to minimise enzyme inactivation, possible interferences from other components, which might be present in the sample (e.g., ascorbic, uric acids) [29][30][31][32] but, on the other hand, still be able to detect the analyte with a high sensitivity a potential of 100 mV vs. pseudo Ag|AgCl (which is formed on the surface of the reference electrode due to the presence of KCl in the working buffer) was chosen for all further experiments. A flow rate of 0.57 mL min −1 provided a good combination of both high and rapid analytical response for the chosen system and was used for further experiments.…”

Section: Resultsmentioning

confidence: 99%

A simple and sensitive method for lactose detection based on direct electron transfer between immobilised cellobiose dehydrogenase and screen-printed carbon electrodes

Safina

Ludwig

Gorton

2010

Electrochimica Acta

View full text Add to dashboard Cite

“…Detection of lactose with biosensors sometimes requires pre-treatment of milk samples to remove fat and proteins [16]. The response of a lactose biosensor may be additionally affected by calcium chloride, ascorbic and/or uric acids [17].…”

mentioning

confidence: 99%

Calculating the output signal parameters of a lactose bienzymatic biosensing system from the transient phase response

Gornischeff¹,

Rinken²

2011

Proc. Estonian Acad. Sci.

View full text Add to dashboard Cite

We constructed for the determination of lactose a bienzymatic biosensing system based on a fibre-optical oxygen sensor and two enzymes -β-galactosidase (β-gal, from Aspergillus oryzae, Sigma Aldrich, EC 3.2.1.23) and glucose oxidase (GOD, from A. niger, Sigma Aldrich, EC 1.1.3.4) and analysed how the calculation of biosensor output signal parameters, used for the calibration of lactose biosensors, is influenced by the data collection period during the transient phase of the signal rising in case no preliminary incubation period with β-gal was applied. The calculation of reaction steady state and kinetic parameters from the biosensor signal revealed that longer data collection periods resulted in more accurate biosensor calibration curves with bigger slopes, while in case of slower reactions the calculated reaction parameters had their maximal values already if data were collected for 600 seconds. For reactions where enzyme concentrations were higher (0.027-0.071 IU/mL β-gal and 2.03-5.33 IU/mL GOD), the steady state signal was not achieved even within 1 hour from the initiation of the reaction and the calculated reaction parameters continued to change. Although the sensor signal was decreasing continuously, the reaction parameters calculated from the transient phase data were suitable for biosensor calibration if the data of at least 500 seconds were taken into consideration.

show abstract

The occurrence and nature of electrochemical activity in milk from a herd of dairy cows

Cited by 6 publications

References 24 publications

An Electrochemical Impedimetric Aptasensing Platform for Sensitive and Selective Detection of Small Molecules Such as Chloramphenicol

An Electrochemical Impedimetric Aptasensing Platform for Sensitive and Selective Detection of Small Molecules Such as Chloramphenicol

A simple and sensitive method for lactose detection based on direct electron transfer between immobilised cellobiose dehydrogenase and screen-printed carbon electrodes

Calculating the output signal parameters of a lactose bienzymatic biosensing system from the transient phase response

Contact Info

Product

Resources

About