The Nutrition of Protozoa

Abstract

City, for a sample of his plasma lipid fraction. For small quantities of human serum albumin, we are indebted to Dr. John Edsall of the Department of Physical Chemistry, Harvard Medical School. Human blood serum from negative Wassermann tests was made available to us through the kindness of Mr. J. H. Spooner of the State Department of Health, Trenton, New Jersey. We are grateful to Dr. Garth Johnson for his generous counsel and encouragement in this investigation.

Influence of Trace Amounts of Fatty Acids on the Growth of Microorganisms

“…Fatty acids, collaborators (38) have demonstrated a peculiar too, were responsible for the growth stimulation effect on spore formers. With anaerobic spore of Trichomonas vaginali (76) and Entamoeba formers a delay in spore fermination was obhistolytica (35) by serum. The detoxifying action served (29) due to unsaturated fatty acids, of blood, on the other hand, has found applicawhereas with aerobic spore formers saturated tion in the use of blood agar for the cultivation fatty acids inhibited the sporulation (38).…”

The Role of Serum in thi Nutrition Asterococcus Mycoides

“…Preliminary work in this Laboratory indicated that lipids were involved in the growth-promoting activity of serum for A. mycoides. Serum extracted by the methods of Hardy and Gardiner (1910), McFarlane (1942), or of Sprince and . Kupferberg (1947) would no longer support growth, but growth was restored in the presence of both the lipid extract emulsified in "Tween 80" as dispersing agent and of the lipid-extracted residue.…”