The nutrition of animal cells in vitro

Paul, John

doi:10.1079/pns19600013

Cited by 4 publications

(3 citation statements)

References 24 publications

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“…One may conclude that vitamin A is required neither for the growth of mammalian mast-cell tumours in culture nor for their synthesis ofheparin. This substantiates an earlier view (Paul, 1960) that fat-soluble vitamins are not required for vertebrate cell growth in vitro. Concurrently with this work J. T. Dingle (personal communication) has found that [14C]glucosamine incorporation by cultured fibroblasts is similarly insensitive to lack of vitamin A.…”

Section: Resultssupporting

confidence: 91%

Vitamin A and the biosynthesis of sulphated mucopolysaccharides. Experiments with rats and cultured neoplastic mast cells

Thomas

Pasternak

1969

Biochemical Journal

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1. The uptake and incorporation of [(35)S]sulphate into mucopolysaccharides by colon and duodenum in vitro are unaffected by the vitamin A status of the animals. 2. Uptake and incorporation in vivo are unaffected at 4hr. after injection of [(35)S]sulphate, but at later times are decreased in some tissues of vitamin A-deficient animals. 3. The rate of removal of (35)S from blood, its rate of appearance in urine, the plasma concentration of sulphate and the uronic acid content of several tissues are not significantly altered in vitamin A deficiency. 4. These results, and direct measurement of (35)S in mucopolysaccharides at various times after injection of [(35)S]sulphate, suggest that the synthesis of mucopolysaccharides is unaffected but that their turnover is increased in vitamin A deficiency. 5. Neither the growth rate of, nor the incorporation of [(35)S]sulphate into heparin by, P815Y and HC cultured neoplastic mast cells is decreased when the horse serum necessary for growth is treated with ultraviolet light or is replaced by serum from vitamin A-deficient rats. 6. The addition of citral is no more toxic to growth rate or to incorporation of (35)S than is the addition of vitamin A itself. 7. It is concluded that neoplastic mast cells in culture do not require vitamin A for growth or for the synthesis of heparin. 8. None of these results is compatible with the view that vitamin A or a derivative is directly involved in the biosynthesis of sulphated mucopolysaccharides.

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Section: Resultssupporting

confidence: 91%

Vitamin A and the biosynthesis of sulphated mucopolysaccharides. Experiments with rats and cultured neoplastic mast cells

Thomas

Pasternak

1969

Biochemical Journal

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“…containing EDTA* in a divalent ion-free salt solution (Paul, 1960) re moves fibroblast-like cells from the glass more readily than epithelial cells. These observations are in sharp contrast to those of Sato et al (1960) and Zaroff et al (1961), who concluded that cells isolated in vitro are derived from a special small minority of the cell population of the tissue of origin.…”

Section: Globulinsmentioning

confidence: 99%

“…The basis for recent developments in the use of mammalian cell cultures as a biological assay method-the definition of the nutritional requirements of mammalian cells in culturehas been reviewed extensively (Eagle, , 1960a; J. F. Morgan, 1958;Swim, 1959;Paul, 1960;Lucy, 1960;Merchant and Eidam, 1961;Morris, 1962;, as has the biochemistry of cultured mammalian cells (Lucy, 1960;Merchant and Eidam, 1961;Morris, 1962;. These collateral studies are considered first in order to provide current background for the evaluation of the usefulness and limitations of these experimental systems as assay tools.…”

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confidence: 99%