The Nucleus-encoded Protein MOC1 Is Essential for Mitochondrial Light Acclimation in Chlamydomonas reinhardtii

Schönfeld, Christine; Wobbe, Lutz; Borgstädt, Rüdiger; Kienast, Alexandra; Nixon, Peter J.; Kruse, Olaf

doi:10.1074/jbc.m408477200

Cited by 106 publications

(117 citation statements)

References 66 publications

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“…Chlamydomonas reinhardtii wildtype wt13 [23] derived from the original Ebersold/Levine line 137c (CC-125 mt+) [24,25] was used as a model organism. For maintenance and preculturing the algae was continuously grown mixotrophic in liquid cultures of TAP media (Tris Acetate Phosphate) [26] under low light intensities (50 µE m -2 ·s -1 ).…”

Section: Strains and Mediummentioning

confidence: 99%

The Application of Transparent Glass Sponge for Improvement of Light Distribution in Photobioreactors

Jacob¹,

Bucharsky²,

GuenterSchell³

2012

J Bioproces Biotechniq

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A new concept for improving light dilution and light distribution in photobioreactors by applying transparent, openpored sponges was realized during cultivation experiments. A manufacturing process based on the polymer replica technique was established. A polyurethane template is impregnated with a nanoscaled SiO 2 powder suspension (solids loading of 60 wt% at around pH 10) and dried. The green body is prepared by burning-out the polymer at 800°C. Subsequently the sintering of the remaining SiO 2 structure to transparent cellular bodies is carried out at 1330°C. Many different factors influence the process. The slurry stabilization (viscosity, zeta-potential, pH) is important to generate a stable and self-supporting SiO 2 shell around the template and achieve appropriate green bodies. The sintering temperature determines the transition of amorphous (transparent) into crystalline silica. The 8 ppi sponge has a porosity of 0.9, the specific surface area of 568 m -1 means an increase of the (inner) surface to volume ratio of the reactor. Porous glass has a very high surface to volume ratio (about 0.26 m 2 /g of glass at 10 µm pore size). Because of multiple and complex reflection of light into the porous glass, the algae can obtain light energy at any location of the total volume. First cultivation experiments in special designed Miniplate reactors show an increase in growth rate (about +25%) at low cell densities. The photo conversion efficiency was enlarged from 4.9% for the empty reactor to 5.6% and 5.9% for the 8 and 15 ppi sponge filled reactor. The effect and the improved efficiency of biomass build-up per applied light will be more apparent at high cell densities. Therefore concentration of the standard Tris-Phosphate (TP) medium (2.5 fold) and feeding are necessary.

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Section: Strains and Mediummentioning

confidence: 99%

The Application of Transparent Glass Sponge for Improvement of Light Distribution in Photobioreactors

Jacob¹,

Bucharsky²,

GuenterSchell³

2012

J Bioproces Biotechniq

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“…At last, the availability of mutants affected to various extents in their ability to couple ATP synthesis to NADH oxidation is also a useful tool to study the relationship between the mitochondrial energetic status of the cell, hydrogen production, carbon metabolism, and ATP/NADPH adjustment for photosynthesis [62,[64][65][66][67] . 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 16 [17], (c) [68], (d) [69], (e) [70], (f) [71], (g) [72], (h) [73], (i) [74], (j) [75], (k) [76], (l) [77], (m) [22], (n) [29], (o) [78], (p) [58], (q) [79], (r)[34] …”

Section: Resultsmentioning

confidence: 99%

“…This mutant named stm6 was obtained by random insertional mutagenesis and is affected in the nuclear MOC1 gene [62]. The Moc1 protein belongs to the mitochondrial transcription Termination Factor (mTERF) family.…”

Section: Mutants Affected In the Transcription Of The Mitochondrial Gmentioning

confidence: 99%

“…complex IV where 28 assembly factors have been identified to date. In addition, the availability of mitochondrial transformation opens the way to reverse genetics and a better knowledge of the small mitochondrial genome of Chlamydomonas.At last, the availability of mutants affected to various extents in their ability to couple ATP synthesis to NADH oxidation is also a useful tool to study the relationship between the mitochondrial energetic status of the cell, hydrogen production, carbon metabolism, and ATP/NADPH adjustment for photosynthesis [62,[64][65][66][67]. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 16 …”

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confidence: 99%

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Respiratory-deficient mutants of the unicellular green alga Chlamydomonas: A review

et al. 2014

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Genetic manipulation of the unicellular green alga Chlamydomonas reinhardtii is straightforward. Nuclear genes can be interrupted by insertional mutagenesis or targeted by RNA interference whereas random or site-directed mutagenesis allows the introduction of mutations in the mitochondrial genome. This, combined with a screen that easily allows discriminating respiratorydeficient mutants, makes Chlamydomonas a model system of choice to study mitochondria biology in photosynthetic organisms. Since the first description of Chlamydomonas respiratory-deficient mutants in 1977 by random mutagenesis, many other mutants affected in mitochondrial components have been characterized. These respiratory-deficient mutants increased our knowledge on function and assembly of the respiratory enzyme complexes. More recently some of these mutants allowed the study of mitochondrial gene expression processes poorly understood in Chlamydomonas. In this review, we update the data concerning the respiratory components with a special focus on the assembly factors identified on other organisms. In addition, we make an inventory of different mitochondrial respiratory mutants that are inactivated either on mitochondrial or nuclear genes. Opposed Reviewers:Claire Remacle -Génétique des microorganismes -Institut de Botanique B22 Boulevard du Rectorat, 27 -Université de Liège-B-4000 Liège, Belgique Tel +32 4 3663812 -Fax +32 4 3663840 e-mail: c.remacle@ulg.ac.be First, we wish to thank the two anonymous reviewers for their constructive comments. We found their suggestions very helpful to improve the quality of our manuscript. We have addressed all the points they raised and have modified the text accordingly. A detailed response to reviewers' comments is included below.We believe we have addressed all referees' comments and we hope this revised version is now suitable for publication in Biochimie.We are looking forward to hearing from you, With best regards, Cover LetterResponse to reviewers The text has been modified in order to give more background details on the methodology used to create and screen respiratory mutants in Chlamydomonas. The main modifications correspond to: Paragraph 2.3: -p8. A figure (Fig. 4) has been added in order to provide more information on dark-dier/slow growth screens for different classes of mutant.-p9. The mitochondrial transformation method used in Chlamydomonas has been detailed.Paragraph 2.4: -p10. The TTC-based screen has been explained.Finally, all along the text the methods used to create nuclear and mitochondrial mutants has been indicated. Also, the text could benefit from being proof-read by a native English speaker as the grammar is a little awkward in places. I highlight a couple of points below, but there are a number of other places where the English could be improved for clarity.The text has been proof-read by a good English speaker as requested and the text has been modified according to the comments.Minor points:1. Abstract, line 1: "the genetics" have been replaced by "genetic manipu...

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“…It promotes transcription termination by binding to a 28-base pair region within the tRNA Leu (UUR) gene at the position immediately adjacent and downstream of the 16S rRNA gene (Kruse et al, 1989). Through bioinformatics tools, its paralogues like sea urchin mtDBP, Drosophila DmTTF, Chlamydomonas reinhardtii Moc1, Mus musculus mTERF, Homo sapiens MTERF2 (mterfd3, mTERFL) and MTERF3 (mterfd1) (Chen et al, 2005;Fernandez-Silva et al, 2001;Li et al, 2005;Roberti et al, 2003Roberti et al, , 2006Schonfeld et al, 2004) with similar structure were found, divided into four subfamilies, and then further characterized. The salient feature of proteins in MTERF family is the containing of variable number of "mterf" motifs of about 30 amino acids in length.…”

Section: Introductionmentioning

confidence: 99%

Expression profile of mouse Mterfd2, a novel component of the mitochondrial transcription termination factor (MTERF) family

Zhang

et al. 2011

Genes Genet. Syst.

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Mterfd2 is a component of mitochondria transcription termination factor (MTERF) family which belongs to the MTERF4 subfamily. In this report, we characterized the expression profile of mouse Mterfd2 during embryogenesis by in situ hybridization (ISH), quantitative real-time PCR (qRT-PCR) and northern blot. The whole mount ISH at E9.5, E10.5 and E11.5 showed that Mterfd2 was dynamically expressed in the brain. Besides, at E9.5 and E10.5 stages, Mterfd2 was persistently expressed in the lateral plate mesoderm and heart; at E10.5 and E11.5 stages, it showed an abundant expression in the limb buds. The tissue ISH of E13.5 and E15.5 suggested that Mterfd2 was ubiquitously expressed, and has the higher expression in the forebrain, diencephalon, midbrain, spinal cord, dorsal root ganglion, tongue, lung, liver and kidney. This ubiquitous expression profile in the late embryogenesis was further confirmed by qRT-PCR and northern blot at E12.5, E15.5 and E18.5 stages. Besides, the results of co-location of EGFPMterfd2 fusion protein indicated that Mterfd2 was targeted to the mitochondria. Collectively, these data suggested that Mterfd2 showed a dynamic expression pattern during embryogenesis. It might play an important role in the organ differentiation which was probably resulted from its role in the mitochondrial transcription regulation.

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The Nucleus-encoded Protein MOC1 Is Essential for Mitochondrial Light Acclimation in Chlamydomonas reinhardtii

Cited by 106 publications

References 66 publications

The Application of Transparent Glass Sponge for Improvement of Light Distribution in Photobioreactors

The Application of Transparent Glass Sponge for Improvement of Light Distribution in Photobioreactors

Respiratory-deficient mutants of the unicellular green alga Chlamydomonas: A review

Expression profile of mouse Mterfd2, a novel component of the mitochondrial transcription termination factor (MTERF) family

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