The nucleotide sequence of the region of bacteriophage T4 inh(lip)-hoc genes

Kaliman, A V; Khasanova, M.A.; Kryukov, V. A.; Tanyashin, V. I.; Bayev, A.A.

doi:10.1093/nar/18.14.4277

Cited by 9 publications

(5 citation statements)

References 2 publications

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“…The td intron homing occurred efficiently even when the length of DNA homology was decreased by up to 35–50 bp [30], while seg E gene mobility did not take place when the downstream DNA homology region was reduced to 73 bp. It is also known that expression of both the I‐TevI ORF and the seg E gene is controlled by T4 late promoters [4, 13]. Thus, although the detailed molecular mechanism by which seg E gene transfer occurs remains to be determined, the process promoted by the SegE endonuclease appears to be associated with the phage recombination‐dependent DNA replication occurring at the late phase of the T4 life cycle [31].…”

Section: Discussionmentioning

confidence: 99%

“…1. The construction of plasmids pVT123, pMX3,4‐2, pVTX and psEET‐3a was described earlier [11, 15, 16]. Plasmids pEW19, pseT219 and pΔA19 contain fragments from the T4 hoc .1‐ uvs W region that were generated by PCR using primer pairs 1+5, 1+2, and 1+3, respectively, and inserted into the Sma I site of pUC19.…”

Section: Methodsmentioning

confidence: 99%

“…The seg E gene of phage T4 lies in the hoc .1‐ uvs W intergenic region and encodes a site‐specific endonuclease [10–13]. SegE and related proteins (SegA–SegD) have a GIY‐YIG motif, which is also present within I‐TevI endonuclease [14].…”

Section: Introductionmentioning

confidence: 99%

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A phage T4 site‐specific endonuclease, SegE, is responsible for a non‐reciprocal genetic exchange between T‐even‐related phages

1997

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The bacteriophage T4 segE gene encoding sitespecific endonuclease lies between the hoc.l and uvsW genes. The similar region of T-even-related phage RB30 lacks the segE gene. Here we demonstrate that the phage T4 segE gene is inherited preferably by progeny of mixed infection with RB30. The preferred inheritance of the segE gene depends on its own expression and is based on a non-reciprocal homologous recombination event providing the transfer of the gene from the segE-containing to the .se^E-lacking alíele. The SegE endonuclease cleaves DNA in a site located at the 5' end of the KVSW gene in the RB30 genome. The T4 DNA is also cleaved by the enzyme, but less efficiently. The cleavage at the RB30 site appears to initiate the observed conversion, which is stimulated by DNA homology and accompanied by co-conversion of flanking markers. Our findings provide a novel example of endonuclease-dependent generation of genetic variation in prokaryotes.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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A phage T4 site‐specific endonuclease, SegE, is responsible for a non‐reciprocal genetic exchange between T‐even‐related phages

1997

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“…The T4 PPase specifically cleaves the 65 residue long amino terminal "∆ piece" of the gp23 molecule [39]. A small piece of the gp24 sequence (2.2 kD) is also cleaved, but the portal protein gp20 is not cleaved.…”

Section: Head Structure and Assemblymentioning

confidence: 99%

Molecular architecture of bacteriophage T4

Mesyanzhinov

Leiman

Kostyuchenko

et al. 2004

Biochemistry (Moscow)

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In studying bacteriophage T4--one of the basic models of molecular biology for several decades--there has come a Renaissance, and this virus is now actively used as object of structural biology. The structures of six proteins of the phage particle have recently been determined at atomic resolution by X-ray crystallography. Three-dimensional reconstruction of the infection device--one of the most complex multiprotein components--has been developed on the basis of cryo-electron microscopy images. The further study of bacteriophage T4 structure will allow a better understanding of the regulation of protein folding, assembly of biological structures, and also mechanisms of functioning of the complex biological molecular machines.

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“…Hoc display vector, pR.hoc. The 5-kb DNA between the T4 map units 107 to 112 kb, consisting of three open reading frames (ORFs) including hoc, 38 kd, and 24, was amplified by PCR with primers 5Ј-CGGGGGATCCAGAGTAGCATG AGCTCCGATG-3Ј and 5Ј-CGGGGGATCCATCATTCAAGTCATCAGTA G-3Ј (12,15). The amplified DNA, after digestion with BamHI was cloned into the BamHI-linearized and dephosphorylated pET-9D (32).…”

Section: Bacteria Escherichia Coli B40 (Supmentioning

confidence: 99%

Display of a PorA peptide from Neisseria meningitidis on the bacteriophage T4 capsid surface

1997

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The exterior of bacteriophage T4 capsid is coated with two outer capsid proteins, Hoc (highly antigenic outer capsid protein; molecular mass, 40 kDa) and Soc (small outer capsid protein; molecular mass, 9 kDa), at symmetrical positions on the icosahedron (160 copies of Hoc and 960 copies of Soc per capsid particle). Both these proteins are nonessential for phage infectivity and viability and assemble onto the capsid surface after completion of capsid assembly. We developed a phage display system which allowed in-frame fusions of foreign DNA at a unique cloning site in the 5 end of hoc or soc. A DNA fragment corresponding to the 36-amino-acid PorA peptide from Neisseria meningitidis was cloned into the display vectors to generate fusions at the N terminus of Hoc or Soc. The PorA-Hoc and PorA-Soc fusion proteins retained the ability to bind to the capsid surface, and the bound peptide was displayed in an accessible form as shown by its reactivity with specific monoclonal antibodies in an enzyme-linked immunosorbent assay. By employing T4 genetic strategies, we show that more than one subtype-specific PorA peptide can be displayed on the capsid surface and that the peptide can also be displayed on a DNA-free empty capsid. Both the PorA-Hoc and PorA-Soc recombinant phages are highly immunogenic in mice and elicit strong antipeptide antibody titers even with a weak adjuvant such as Alhydrogel or no adjuvant at all. The data suggest that the phage T4 hoc-soc system is an attractive system for display of peptides on an icosahedral capsid surface and may emerge as a powerful system for construction of the next generation multicomponent vaccines.

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The nucleotide sequence of the region of bacteriophage T4 inh(lip)-hoc genes

Cited by 9 publications

References 2 publications

A phage T4 site‐specific endonuclease, SegE, is responsible for a non‐reciprocal genetic exchange between T‐even‐related phages

A phage T4 site‐specific endonuclease, SegE, is responsible for a non‐reciprocal genetic exchange between T‐even‐related phages

Molecular architecture of bacteriophage T4

Display of a PorA peptide from Neisseria meningitidis on the bacteriophage T4 capsid surface

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