The nucleotide sequence of the 5′-untranslated region of bovine viral diarrhoea virus: its use as a probe in rapid detection of bovine viral diarrhoea viruses and border disease viruses

Qi, Fengxia; Gustad, Thomas R.; Lewis, Terry L.; Berry, Eugene S.

doi:10.1006/mcpr.1993.1052

Cited by 16 publications

(4 citation statements)

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“…The 5′ non-coding region (5′NCR) of the genome is considered to be highly conserved among pestiviruses, allowing the selection of specific primers that amplify all known pestiviruses. Therefore, the 5′NCR has been the target region when studying differences between and within pestiviruses [8,13,14,23,25]. Reverse transcriptase polymerase chain reaction (RT-PCR) has been used by various researchers for the detection of pestiviruses using oligonucleotide primers based on the 5′NCR of the viral genome [16][17].…”

Section: Introductionmentioning

confidence: 99%

Genetic characterization of bovine viral diarrhoea (BVD) viruses: confirmation of the presence of BVD genotype 2 in Africa

et al. 2012

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Bovine viral diarrhoea virus (BVDV) has emerged as one of the economically important pathogens in cattle populations with a worldwide distribution causing a complex of disease syndromes. Two genotypes BVDV 1 and 2 exist and are discriminated on the basis of the sequence of the 5′ non-coding region (5 NCR) using real-time PCR. The use of the real-time PCR is more sensitive, specific, and less time consuming than a conventional PCR, and has reduced the risk of cross-contamination of samples. Limited information exists on BVDV genetic subtypes in South Africa. The aim of this study was to determine the genotypes of

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Section: Introductionmentioning

confidence: 99%

Genetic characterization of bovine viral diarrhoea (BVD) viruses: confirmation of the presence of BVD genotype 2 in Africa

et al. 2012

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“…The genus also includes classical swine fever virus (CSFV) and Border disease virus (BDV) of sheep [3,20]. Based on a comparison of the 5' untranslated region (UTR) and the N pro -and E2-encoding sequences [23,30], BVDV can be divided into two different genotypes: BVDV-1 and BVDV-2 [21,22]. The genome of each genotypes contains a single positivestranded RNA with a size of approximately 12.3 kb, consisting of a single large open reading frame (ORF) flanked by 5' and 3' untranslated regions [6,8].…”

Section: Introductionmentioning

confidence: 99%

Analysis of codon usage in bovine viral diarrhea virus

et al. 2010

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Bovine viral diarrhea virus (BVDV) is a widespread virus in beef and dairy herds. BVDV has been grouped into two genotypes, genotype 1 and genotype 2. In this study, the relative synonymous codon usage (RSCU) values, effective number of codon (ENC) values and nucleotide content were investigated, and a comparative analysis of codon usage patterns for open reading frames (ORFs) of 22 BVDV genomes, including 14 of genotype 1 and 8 of genotype 2, was carried out. A high A+U content and low codon bias were found in BVDV genomes. Depending on the RSCU data, it was found that there was a significant variation in bias of codon usage between the two genotypes, and a geographic factor exists only in genotype-1 of BVDV. The RSCU data have a negative correlation with general average hydrophobicity (GRAVY), aromaticity and nucleotide content. Furthermore, the overall abundance of C and U has no effect on the synonymous codon usage patterns. In contrast, the A and G content showed a significant correlation with the nucleotide content at the third position. In addition, the codon usage patterns of BVDV are similar to those of 22 conserved genes of Bos taurus. Taken together, the genetic characteristics of BVDV possibly result from interactions between natural selection and mutation pressure.

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“…The nucleotide sequence of the 5'-UTR is very similar and well conserved among the members of the Pestivirus genus [29,35], thus providing a useful region suitable for accurate virological negative 23 rubella negative negative 24 rubella negative negative 25 rubella negative negative 26 rubella negative negative 27 polio negative negative 28 polio negative negative 29 polio negative negative 30 polio negative negative 31 polio negative negative 32 polio negative negative 33 polio negative negative 34 polio negative negative 35 polio negative negative 36 polio negative negative 37 polio negative negative 38 Str. pneumoniae* negative negative *: Bacterial vaccine.…”

Section: Discussionmentioning

confidence: 99%

Genotypes of Pestivirus RNA Detected in Live Virus Vaccines for Human Use.

Giangaspero

Vacirca

Harasawa

et al. 2001

The Journal of Veterinary Medical Science

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ABSTRACT. Live virus vaccines for human use, 29 monovalent vaccines against measles, mumps, rubella or polio, eight polyvalent vaccines against measles-mumps-rubella and one bacterial polyvalent vaccine against Streptococcus pneumoniae, were tested by reverse transcriptase-nested PCR for the presence of petivirus or pestivirus RNA. Twenty-four samples were selected from European manufacturers, ten were from U.S.A. and four from Japan. Five (13.1%) out of 38 tested samples were positive for pestivirus RNA. Three vaccines (rubella and two measles) were from Europe and two (mumps and rubella) from Japan. The 5'-untranslated genomic region of the contaminant pestivirus RNA were amplified by reverse transcription-PCR and sequenced. Analyses based on primary nucleotide sequence homology and on secondary structures, characteristic to genotypes, revealed that the cDNA sequences belonged to bovine viral diarrhea virus (BVDV). A cDNA sequence, detected from one measles sample, belonged to BVDV-1b genotype. Pestiviral cDNA detected from the Japanese mumps and rubella vaccine samples, belonged to the BVDV genotypes 1a and 1c, respectively. Analysis on two cDNA sequences detected from measles and rubella vaccine samples from Europe showed their appurtenance to a new genotype, BVDV-1d. These findings indicate that contamination by animal pestivirus may occur in biological products for human use.

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The nucleotide sequence of the 5′-untranslated region of bovine viral diarrhoea virus: its use as a probe in rapid detection of bovine viral diarrhoea viruses and border disease viruses

Cited by 16 publications

References 0 publications

Genetic characterization of bovine viral diarrhoea (BVD) viruses: confirmation of the presence of BVD genotype 2 in Africa

Genetic characterization of bovine viral diarrhoea (BVD) viruses: confirmation of the presence of BVD genotype 2 in Africa

Analysis of codon usage in bovine viral diarrhea virus

Genotypes of Pestivirus RNA Detected in Live Virus Vaccines for Human Use.

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