The nucleotide sequence of barley strain of<i>Wheat Dwarf Virus</i>isolated in Bulgaria

Four Hungarian winter wheat cultivars were investigated for their susceptibility to the geminivirus Wheat dwarf virus (WDV). Previously, two cultivars (Mv Regiment and Mv Emese) were assessed by breeders to exhibit virus symptoms in the field, whereas Mv Dalma and Mv Vekni showed few symptoms. Two inoculation techniques for WDV, vector transmission with the leafhopper Psammotettix alienus and agroinoculation, were used. Leafhopper transmission was more efficient than agroinoculation. However, irrespective of the technique used, no Mv Dalma or Mv Vekni plants showed clear WDV symptoms. In contrast, 3 ⁄ 30 Mv Emese and 4 ⁄ 36 Mv Regiment plants showed dwarfing and chlorosis after agroinoculation and 13 ⁄ 17 and 14 ⁄ 15 plants, respectively, had clear WDV symptoms after vector transmission. WDV-specific PCR showed that Mv Vekni and Mv Dalma plants could be infected, especially following vector transmission (approximately 50% infection), but at significantly lower frequency than Mv Emese or Mv Regiment plants (100% infection). Furthermore, real-time PCR showed that WDV DNA accumulated to much lower levels in infected Mv Vekni and Mv Dalma plants than in infected Mv Regiment and Mv Emese plants. The data strongly suggest that Mv Vekni and Mv Dalma are partially resistant to WDV infection. As WDV resistance has not previously been identified in wheat, and because WDV can cause significant yield losses, the resistance of Mv Vekni or Mv Dalma will provide a valuable breeding resource.

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“…In addition, WDV has recently been reported from Turkey (Koklu et al. , 2007), Bulgaria (Tóbiás et al. , 2009), Africa (Najar et al.…”

Section: Introductionmentioning

confidence: 98%

Partial resistance to Wheat dwarf virus in winter wheat cultivars

et al. 2010

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“…ere are, however, contradictory reports on whether the wheat strain can infect barley, and whether the barley strain can infect wheat. Lindsten and Vacke (1991) and Tóbiás et al (2009) observed no transmission of the barley strain to wheat, whereas the wheat strain was transmissible to barley. Similar conclusion was made by Kundu et al (2009), who found that the barley strain is restricted to the barley host, while the wheat strain is present in both wheat and barley plants.…”

Section: Comparison Of the Nucleotide Sequences Of Wheat Dwarf Virus mentioning

confidence: 98%

Comparition of the Nucleotide Sequences of Wheat Dwarf Virus (WDV) Isolates from Hungary and Ukraine

TÓBIÁS¹,

Shevchenko²,

KISS³

et al. 2011

Pol J Microbiol

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Wheat dwarf virus (WDV) is the most ubiquitous virus in cereals causing huge losses in both Hungary and Ukraine. The presence of barley-and wheat-adapted strains has been confirmed, suggesting that the barley strain is restricted to barley, while the wheat strain is present in both wheat and barley plants. Five WDV isolates from wheat plants sampled in Hungary and Ukraine were sequenced and compared with known WDV isolates from GenBank. Four WDV isolates belonged to the wheat strain. Our results indicate that WDV-Odessa is an isolate of special interest since it has originated from wheat, but belongs to the barley-adapted strain, providing novel data on WDV biology and raising issues of pathogen epidemiology.

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