“…Our understanding of how sequence-non-specific chromatin remodeling complexes achieve specificity at genomic loci is still developing. Prior work has uncovered myriad remodeler-targeting ‘cues,’ including post-translational histone modifications (Clapier et al, 2001; Dann et al, 2017; Mashtalir et al, 2021), TFs (Barisic et al, 2019; Brahma and Henikoff, 2019; de Dieuleveult et al, 2016), three-dimensional (3D) chromosomal architecture (Barisic et al, 2019; Barutcu et al, 2016; Weber et al, 2021), composition of the nucleosome core particle (Dann et al, 2017; Gamarra et al, 2018; McBride et al, 2020), and, as discussed here, availability of extranucleosomal flanking DNA (Stockdale et al, 2006; Yang et al, 2006; Zofall et al, 2004). Here, we elaborate on the length-sensing model by connecting DNA length-sensing on mononucleosomes to nucleosome density of individual chromatin fibers.…”