The Nucleic Acid and Carbohydrate of Influenza Virus

Knight, C.A.

doi:10.1084/jem.85.1.99

Cited by 57 publications

(10 citation statements)

References 30 publications

(56 reference statements)

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“…Though Knight (1947) found that a sample of nucleic acid isolated from defatted virus was degraded by crystalline ribonuclease, specific chemical evidence has been obtained only recently. As judged by absorption measurements at 260 mp.…”

mentioning

confidence: 99%

Influenza Virus Nucleic Acid: Relationship between Biological Characteristics of the Virus Particle and Properties of the Nucleic Acid

Ada

Perry

1956

Journal of General Microbiology

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mentioning

confidence: 99%

Influenza Virus Nucleic Acid: Relationship between Biological Characteristics of the Virus Particle and Properties of the Nucleic Acid

Ada

Perry

1956

Journal of General Microbiology

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“…The Public Health Laboratory, Northampton (With 4 Figures in the Text) Early estimations of the nucleic acid content of influenza virus A gave values of the order of 5 % and indicated the probable presence of both ribonucleic acid (RNA) and deoxyribonucleic acid (DNA) (Taylor, 1944;Knight, 1947;Graham, 1950).…”

Section: By W Frisch-niggemeyer and L Hoylementioning

confidence: 99%

“…Taylor (1944) and Knight (1947) showed that influenza virus preparations contained carbohydrate other than nucleic acid sugar. By the use of the carbazole colour reaction Knight suggested that the carbohydrate was made up of galactose, mannose and glucosamine units.…”

Section: Nucleic Acid Content Of Intact Virusmentioning

confidence: 99%

The nucleic acid and carbohydrate content of influenza virus A and of virus fractions produced by ether disintegration

Frisch-Niggemeyer¹,

Hoyle²

1956

J. Hyg.

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The preparation of purified influenza virus from infected allantoic fluid is described, and its fractionation by ether treatment with the production of pure samples of soluble antigen and haemagglutinin.Chemical analysis of intact infective virus showed it to have a nitrogen content of 9.3%, a carbohydrate content of 3.5%, and a content of ribonucleic acid of 0.7–0.74%.Purified soluble antigen derived from the infective virus particle was shown to be a ribonucleoprotein containing 5.3% RNA. No carbohydrate other than ribose was found. Purified haemagglutinin was shown to be a mucoprotein containing 4.2% of a polysaccharide possibly composed of galactose and mannose in equimolar proportions.

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“…There are sixteen amino acids in all (Knight, 1947), the major one being arginine 9-8 per cent, the others are lysine 1-47 per cent, aspartic acid 13-5 per cent, glutamic acid 11-3 per cent, which accounts for the acidity of the protein, cysteine (no cystine) 0-69 per cent, glycine 1-9 per cent, isoleucine &6 per cent, leucine 9-3 per cent, phenylalanine 8 -4 per cent, proline 5-8 per cent, serine 7-2 per cent, threonine 9-9 per cent, tryptophane 2-1 per cent, tyrosine 3-8 per cent, valine 9-2 per cent.…”

Section: Tobacco Mosaic Virusmentioning

confidence: 99%

“…Investigations on highly purified preparations of influenza virus have revealed the presence of an amount of carbohydrate apparently greater than that accountable for in the nucleic acids. Knight (1947) obtained carbohydrate-rich fractions from highly purified samples of PR 8 and Lee influenza viruses and subjected them to analysis. In each case, the carbohydrate appeared to be a polysaccharide composed of mannose, galactose, and glucosamine units.…”

Section: Influenza Virusmentioning

confidence: 99%

An Introduction to the Study of Viruses

1951

JAMA

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At the time of writing this book, there has been no attempt to present the study of viruses as the separate discipline of-to use a bastard word-virology. Heretofore, the subject has been divided up into artificial compartments, plant viruses, animal viruses, and bacteriophages, as if there were no common bond between them. I have therefore attempted to survey the whole field, superficially no doubt, but in such a way that the relationships between these interesting agents may become clear to the student. In doing this I am aware that I am inviting criticism and that there may be little in common between, say, the viruses of vaccinia or psittacosis and some of the very small plant viruses. We can, however, follow Boycott's suggestion of a descending scale with the smallest organisms at one end and the protein molecule at the other. In between those limits, and perhaps overlapping at each end, lie the viruses, a heterogeneous collection no doubt but all showing-some characteristics in common. Like most modern scientific disciplines, but more so than some, the study of viruses becomes essentially a matter of team work because of its many contacts with other subjects and its wide ramifications through much of the field of natural science. This makes it a difficult matter for one writer to cover the field in an adequate, if only introductory, manner and a severe test of his comprehension of the subject. I have, nevertheless, written this book in the hope that it will be of use, not only to the 'Virologist," if such a person exists, but also to the biochemist, the biologist, the medical student and anyone else who is interested in that borderland country whose boundaries were described by Aristotle as "indistinct and doubtful." Grateful acknowledgment is due to my colleague, Dr. Roy Markham, for his constructive criticism of Chapters V to VIII, and for taking many o£ the photographs used in the illustrations.

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The Nucleic Acid and Carbohydrate of Influenza Virus

Cited by 57 publications

References 30 publications

Influenza Virus Nucleic Acid: Relationship between Biological Characteristics of the Virus Particle and Properties of the Nucleic Acid

Influenza Virus Nucleic Acid: Relationship between Biological Characteristics of the Virus Particle and Properties of the Nucleic Acid

The nucleic acid and carbohydrate content of influenza virus A and of virus fractions produced by ether disintegration

An Introduction to the Study of Viruses

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