The nuclease activity of the endogenous differentiation factor of the HL-60 cell line

Dranitsyna, S. M.; Коstanyan, I. A.; Andreeva, Svetlana; Astapova, M. V.; Бабиченко, И. И.; Baeva, Olga V.; Bogachuk, A. P.; Molotkovskaya, I. M.; Rodionov, I. L.; Smirnova, E. V.; Липкин, В. М.

doi:10.1007/bf02759282

Cited by 4 publications

(5 citation statements)

References 35 publications

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“…HLDF-24 probably acts as an ECE antagonist and suppresses the formation of endogenous endothelin. HLDF-8 exhibits DNA/ RNA-hydrolyzing activity, which promotes free radical generation in the vascular bed [2]. Free radicals inactivate various enzymes, including Znmetalloproteinases (e.g., ECE).…”

Section: Resultsmentioning

confidence: 99%

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Hemodynamic effects of peptide fragments of differentiation factor HLDF

Ржевский

Dranitsyna²,

Байдакова

et al. 2006

Bull Exp Biol Med

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Section: Resultsmentioning

confidence: 99%

“…This protein was found in the blood and nervous system of mammals [14]. This factor contained two active fragments: hexapeptide HLDF-6 possessing differentiation capacity of the full-length factor and exhibiting protective activity [1,3,5,6,13] and octapeptide HLDF-8 exhibiting apoptogenic activity [2].…”

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confidence: 99%

Hemodynamic effects of peptide fragments of differentiation factor HLDF

Ржевский

Dranitsyna²,

Байдакова

et al. 2006

Bull Exp Biol Med

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“…It induces differentiation of actively proliferating HL-60 cells and participates in apoptosis processes. Two active fragments of HLDF are identified: six-member peptide fragment (HLDF-6) completely retaining differentiating activity of the initial protein and possessing antiapoptotic and cell-protective properties and eightmember peptide (HLDF-8) capable of inducing programmed cell death [1,2]. HLDF is present in mammalian and human blood and nervous system, while the peptide fragments of this protein are essential for cognitive functions [14].…”

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confidence: 99%

Experimental model of hemorrhagic stroke: Rabbit immunization with HL-60 promyelocytic cell differentiation factor

et al. 2006

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“…Among the known agents, mention should be made of the hemin conjugates with peptides (hemin peptides) we synthesized previously [3], in particular, those described by the formula The peptide components of hemin peptides are fragments of the HL-60 cell line of human promyelocyte leukemia differentiating factor (HLDF) [2].…”

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“…A substantial number of artificial nucleases, which are compounds of various classes or conjugates, are also known [1,2].…”

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confidence: 99%

Interaction of Artificial Nuclease and DNA: Atomic Force Microscopy Data

et al. 2005

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The interest in studies of nucleic acid cleavage under the action of nucleases is largely related to the development of new systems for solving problems of genetic engineering and to the search for new-generation therapeutic drugs.In this work, the highly efficient cleavage of plasmid DNA by original synthetic biodegradable and biocompatible nucleases, conjugates of natural products, was studied by different physicochemical methods. The invoking of atomic force microscopy (AFM) as an additional method allowed us to improve visualization and find reliable evidence for full nonspecific cleavage of the nucleic acid under the action of the artificial nucleases and to interpret more correctly the data of gel electrophoresis and electronic spectroscopy.The destruction of nucleic acids can proceed enzymatically, i.e., under the action of the extensive group of nucleases of natural origin. A substantial number of artificial nucleases, which are compounds of various classes or conjugates, are also known [1, 2].The search for and design of biocompatible and biodegradable compounds able to perform highly efficient destruction of DNA and RNA are currently under way. Among the known agents, mention should be made of the hemin conjugates with peptides (hemin peptides) we synthesized previously [3], in particular, those described by the formula N N N N Fe 3+ COOH COX Cl -(OH) (X) (I) X = -ArgArgTrpHisArgLeuLysGlu(OMe)OH (II) X = -ArgTrpHisArgLeuLysGlu(OMe)OH (III) X = -TrpHisArgLeuLysGlu(OMe)OH The peptide components of hemin peptides are fragments of the HL-60 cell line of human promyelocyte leukemia differentiating factor (HLDF) [2].The data on the nuclease activity of compounds I -III were obtained by the traditional method, namely, gel electrophoresis in agarose gel with coloring of various forms of the plasmid DNA pGem by a fluorescent intercalating dye, ethidium bromide [3, 4] (Fig. 1). The plasmid DNA pGem is a two-strand ring molecule (3700 base pairs).Hemin peptides I -III are able to cleave all forms of plasmid DNA, the cleavage efficiency and conditions (hemin peptide concentration, pH, and medium composition) depending on their structure.

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The nuclease activity of the endogenous differentiation factor of the HL-60 cell line

Cited by 4 publications

References 35 publications

Hemodynamic effects of peptide fragments of differentiation factor HLDF

Hemodynamic effects of peptide fragments of differentiation factor HLDF

Experimental model of hemorrhagic stroke: Rabbit immunization with HL-60 promyelocytic cell differentiation factor

Interaction of Artificial Nuclease and DNA: Atomic Force Microscopy Data

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