The Novel ps and ps-2 Specific Markers for Selection of Functional Male Sterile Tomato Lines in Breeding Programs and Hybrids Seed Production

Abstract

<p>Functional male sterility in tomato (<em>Solanum lycopersicum</em> L.), controlled by the <em>ps-2</em> and <em>ps</em> genes can be utilized in the production of F₁hybrid tomato seed. Two novel cleaved amplified polymorphic sequence (CAPS) markers linked to the <em>ps-2</em> and <em>ps</em> genes were found in tomato. The C4-30 and C2-21 markers were developed based on the conserved ortholog set II (COSII) sequences C2_At3g20020 and C2_At1g65900 located on tomato chromosomes 4 and 2, respectively. The <em>Hin</em>fI-derived PCR restriction product C4-30₈₀<em> </em>was applicable in the detection of functional male sterile plants. In case of the C2-21 marker, a polymorphism was revealed after digestion of the amplicon with restriction enzyme <em>Mbo</em>I. Specificity of the DNA markers identified was verified by scoring the tomato parental lines, F₂ progeny and F₁ hybrids, in which maternal lines possessed the <em>ps</em> or <em>ps-2</em> gene. C4-30 and C2-21 can be used as the diagnostic tools in tomato breeding programs and in F₁ hybrid seed production.</p

Prospects for marker-associated selection in tomato <i>Solanum lycopersicum</i> L.

“…The single mutation that disrupts splicing of mRNA, resulting in its aberrant forms is responsible for the ps2 phenotype. A number of markers have been developed for the Ps-2 gene: SNP (Gorguet et al, 2009), CAPS (Staniaszek et al, 2012), etc.…”

Strategies for Improvement of Horticultural Crops against Abiotic Stresses

“…Further, classical breeding faced some difficulty affected the hybridization such as environmental conditions in particular, low and high relative humidity, as well as low and high temperatures [5]. Furthermore, selection is the most basic and ancient procedure in plant breeding program.…”