Prospects for marker-associated selection in tomato &lt;i&gt;Solanum lycopersicum&lt;/i&gt; L.

Shcherban, A. B.

doi:10.18699/vj19.522

Cited by 3 publications

(1 citation statement)

References 61 publications

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“…Despite the importance of varietal certification and assessment of intervarietal genome variability, there are few studies on marking the genotypes of tomato cultivars in Russia, and these are mainly works on genotyping using already known markers (Shcherban, 2019). For example, a collection of tomato varieties and hybrids from the Michurinsky State Agrarian University was screened using the P7 molecular marker to identify donors of cladosporiosis resistance (Shamshin et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Genome variability of domestic tomato varieties: data from AFLP analysis

et al. 2022

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Tomato Solanum lycopersicum L. is one of the main vegetable crops, accessions and cultivars of which are characterized by a low level of genomic polymorphism. Introgressive tomato breeding uses related wild Solanum species to improve cultivars for stress tolerance and fruit quality traits. The aim of this work was to evaluate the genome variability of 59 cultivars and perspective breeding lines of S. lycopersicum and 11 wild tomato species using the AFLP method. According to the AFLP analysis, four combinations of primers E32/M59, E32/M57, E38/M57, and E41/M59, which had the highest PIC (polymorphism information content) values, were selected. In the process of genotyping a collection of 59 cultivars/lines of S. lycopersicum and 11 wild tomato accessions, the selected primers revealed 391 fragments ranging in size from 80 to 450 bp, of which 114 fragments turned out to be polymorphic and 25 were unique. Analysis of the amplif ication spectra placed wild tomato accessions into separate clades. Sister clades included cultivars of FSCV breeding resistant to drought and/or cold and, in part, to late blight, Alternaria, Septoria, tobacco mosaic virus and blossom end rot, as well as tomato accessions not characterized according to these traits, which suggests that they have resistance to stress factors. In accessions of distant clades, there was clustering on the basis of resistance to Verticillium, cladosporiosis, Fusarium, tobacco mosaic virus, gray rot, and blossom end rot. The combination of accessions according to their origin from the originating organization was shown. The primer combinations E32/M59, E32/M57, E38/M57 and E41/M59 were shown to be perspective for genotyping tomato cultivars to select donors of resistance to various stress factors. The clade-specif ic fragments identif ied in this work can become the basis for the development of AFLP markers for traits of resistance to stress factors.

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Section: Introductionmentioning

confidence: 99%

Genome variability of domestic tomato varieties: data from AFLP analysis

et al. 2022

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Technologies of DNA marking of target genes as a tool for selecting parental pairs in tomato breeding programs for resistance to Fusarium oxysporum f. ssp. Lycopersici.

Korzh

Dubina

2022

BIO Web Conf.

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Development of tomato varieties and hybrids with resistance to the pathogen Fusarium oxysporum f sp. Lycopersici is a priority in breeding of vegetable crops. Evaluation and selection of the initial material is important in effective breeding for resistance to a pathogen. To do this, along with classical methods in tomato breeding, the method of molecular marking is widely used. The purpose of this study was to screen the existing collection of tomatoes with molecular markers I-2/5, I2OH and At-2, which allow identification of resistance genes to the first and second races of the fusarium pathogen. As a result of the work, genotypes were identified that can be used as donors of resistance genes for further breeding work.

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Estimation of the prospects of using agrobiotechnologies to increase yield and preservation of soils

Startsev

Glinushkin

Spiridonov

2021

IOP Conf. Ser.: Earth Environ. Sci.

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The article substantiates the possibility of creating agrobiocenoses with strategically important crops for the formation of a nutritional balance in the diet of the population of the Russian Federation without the use of chemically synthesized agrochemicals, in which the variety of the main elements of biologic technologies of cultivation of agricultural plants plays a key role. Progressive analysis of demand from the agro-industrial complex, indicators of the development of the agrobiotechnology market, consumer trends, and venture investments in biologic technologies allow us to conclude that the development and proposed approaches are promising, allowing to obtain high yields of agricultural crops while maintaining and increasing soil fertility, improving the phytosanitary state of fields.

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Prospects for marker-associated selection in tomato <i>Solanum lycopersicum</i> L.

Cited by 3 publications

References 61 publications

Genome variability of domestic tomato varieties: data from AFLP analysis

Genome variability of domestic tomato varieties: data from AFLP analysis

Technologies of DNA marking of target genes as a tool for selecting parental pairs in tomato breeding programs for resistance to Fusarium oxysporum f. ssp. Lycopersici.

Estimation of the prospects of using agrobiotechnologies to increase yield and preservation of soils

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