The Novel Mesoporous Silica Aerogel Modified With Protic Ionic Liquid for Lipase Immobilization

Abstract

Recebido em 24/08/2015; aceito em 22/12/2015; publicado na web em 11/02/2016Mesoporous silica supports (aerogels) were used to immobilize Burkholderia cepacia lipase (BC) by encapsulation (EN or ENIL), physical adsorption (ADS or ADSIL) and covalent binding (CB or CBIL) into or onto the aerogel modified with protic ionic liquid (PIL). Yield immobilization (Ya) and operational stability were determined by the hydrolytic reaction of olive oil. Ya (37% to 83% by physical adsorption) and operational stability (2 to 23 batches by encapsulation) increased when the support was modified with PIL. For immobilized derivates observed by the BET method, in this case ADS and CB for ADSIL and CBIL, increased pores size was observed, possibly due to the higher amount of BC immobilized conferring Ya and operational stability. This effect was probably attributed to the entry of the enzyme into the pores of the silica aerogel structure. SEM images showed a change in the structure and properties of immobilized lipase derived with PIL. A characteristic FTIR band was obtained for the silanol groups and amides I, IV and V, demonstrating the efficiency of immobilization of BC. The most efficient biocatalysts were ADSIL with regard to yield immobilization and ENIL for operational stability.

Determinação Do Fator De Efetividade Para Enzimas Imobilizadas Usando Métodos De Regressão Simbólica via Programação Genética

“…A medida da tensão superficial ao longo do tempo é um método indireto para monitorar a produção de biossurfactante sendo, desta forma, de grande importância no estudo desse bioproduto. À medida que o microrganismo cresce, o biossurfactante é sintetizado e excretado no meio, reduzindo a tensão superficial (LIMA et al, 2016).…”

“…Por esse motivo a avaliação da caracterização físico-química aliada à caracterização bioquímica destes sistemas imobilizados se intensificou na última década. Os biocatalisadores imobilizados em suportes inorgânicos já foram estudados por diversos grupos (BARBOSA et al 2014;OLIVEIRA et al, 2014;BARBOSA et al 2016), mas poucos relatam a imobilização em suportes orgânicos.…”

“…A técnica tem aplicações industriais com base em métodos químicos e físicos, tais como a evaporação do solvente, polimerização interfacial, e da armadilha (orgânico/ inorgânico) da matriz (KATO et al, 2011). Atualmente, dispõe-se de um grande número de matrizes, englobando materiais orgânicos e inorgânicos, naturais ou sintéticos (RUZENE et al, 2014, BARBOSA et al, 2014WANG et al, 2015;BARBOSA et al, 2016;MAJEWSKI et al, 2017).…”

Lipase Immobilization on Silica Xerogel Treated with Protic Ionic Liquid and its Application in Biodiesel Production from Different Oils

“…Efficient immobilization is the result of the perfect matching of several factors depending on the enzyme, the process, the immobilization support, and the additives used for modification supports [ 11 , 12 , 13 , 14 ]. Therefore, the use of an appropriate support can influence the biocatalyst physical-chemical properties, such as diffusion and catalytic efficiency on each specific reaction system [ 15 , 16 ].…”

“…An alternative method for support enhancement in enzymatic immobilization is the modification of a silica surface support using an ionic liquid as an additive. The application of an additive has as its main goals a positive influence in the gel structure modification, the addition of silanol groups on the surface, an increase in surface area and pore size and the enhancement of the protective hydration layer on the enzyme, which prevents enzyme denaturation in the presence of alcohol [ 12 , 13 , 15 , 18 , 19 ].…”