The renal system is one of the major organ systems present in the human body, it is responsible for maintaining electrolyte balance, secreting important hormones, and maintaining homeostasis in the body. Every individual has 2 kidneys which are located behind the peritoneum at the level of L2-L3 from the vertebral column. Broadly kidneys function to filter blood, purify it, and eliminate toxic waste. We propose a diagnostic test that evaluates the renal blood flow rate in the kidneys and assesses their functioning based on their hemodynamics in the given time frame. Here we aim to explore the application of nanoparticles to assess localized blood pressure in the kidney based on the illumination intensity/radioactivity. Connexin40(Cx40) is a transmembrane protein present in juxtaglomerular apparatus, Cx40 gold antibodies prepared can be conjugated with nanoparticles and introduced in renal circulation. Imaging technologies such as fluorescence/computed tomography can be used to detect these nanoparticles. Once injected they can be assessed and information on renal blood flow can be obtained. The intensity generated on screening can be used for the assessment of renal blood pressure. This information will be valuable for the assessment of kidney function.