The Nonsteroidal Antiinflammatory Drug Diclofenac Potentiates the In Vivo Activity of Caspofungin Against Candida albicans Biofilms

Bink, Anna; Kucharíková, Soňa; Neirinck, Bram; Zhang, Fei; Dijck, Patrick Van; Cammue, Bruno P. A.; Thevissen, Karin

doi:10.1093/infdis/jis594

Cited by 62 publications

(56 citation statements)

References 20 publications

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“…Sodium Diclofenacshowed lower MIC against C. albicans and C. glabrata while ibuprofen showed lower MIC against C. krusei in comparison to other NSAIDs. By testing their effect on germ tube formation, it was found that Sodium Diclofenacat 500 µg/ml inhibit germ tube formation while ketoprofen and ibuprofen inhibit it to lesser extent that agree with many studies [40,41,42].…”

Section: Discussionsupporting

confidence: 86%

“…The second study [42] showed inhibition of the yeast-to-hyphae transition by diclofenac on solid YPD media. The first study demonstrated a significant reduction of biofilm formation in YPD on small polyvinyl chloride disks by diclofenac when added during the period of adhesion and biofilm formation, quantified by XTT measurements.…”

Section: Discussionmentioning

confidence: 97%

“…Ghalehnoo et al, [41] and Bink et al, [42] have documented the effect of diclofenac on C. albicans biofilms or on cells grown on solid media. The second study [42] showed inhibition of the yeast-to-hyphae transition by diclofenac on solid YPD media.…”

Section: Discussionmentioning

confidence: 99%

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Effect of Some Non steroidal Anti-Inflammatory Drugs on Growth, Adherence and Mature Biofilms of Candida spp.

Ashraf¹,

Yousri²,

Taha³

et al. 2015

AJMR

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Candida spp. are the most common cause of fungal diseases and the fourth commonest cause of nosocomial invasive infections which are considered in many cases as life threatening. Among Candida spp., C. albicans is the most common cause of many fungal diseases, but non-albicans spp. infections are in increase. Nonsteroidal anti-inflammatory drugs have previously been shown to have antifungal activity. In this study we determine the antifungal activity of the tested NSAIDs using agar well diffusion method, their effect on the dimorphic transition of C. albicans by testing their ability to form germ tube in the presence of human serum. Determining the effect of NSAIDs on the adherence to plastic surfaces and on the mature biofilms formed by the tested Candida spp.. The results indicated that Sodium Diclofenac showed lower MIC against C. albicans and C. glabrata while Ibuprofen had lower MIC against C. krusei. upon examining the effect of Diclofenac sodium, Ibuprofen and ketoprofen on biofilm formed on polyurethane segments by Scanning electron microscope (SEM), a damage to membranes of the tested species was observed. Sodium Diclofenac showed the highest inhibitory effect on the adherence (51.1-76.9% at MIC and 56.6-83.3% at 2XMIC) of C. albicans and C. glabrata but Ibuprofen showed a higher inhibitory effect against the adherence of C. krusei. For mature biofilms, the highest disruptive effect on mature biofilms formed by all tested Candida Spp. .06% at 2XMIC) was observed by Diclofenac sodium. Sodium Diclofenac inhibited dimorphic transition of C. albicans but a decrease in germ tube formation was shown by others. In conclusion, the tested drugs showed antifungal, anti-adherent and antibiofilm activity that make them useful in the treatment of fungal infection and the prevention of biofilm formation on the surface of medical devices.

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Section: Discussionsupporting

confidence: 86%

Section: Discussionmentioning

confidence: 97%

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Effect of Some Non steroidal Anti-Inflammatory Drugs on Growth, Adherence and Mature Biofilms of Candida spp.

Ashraf¹,

Yousri²,

Taha³

et al. 2015

AJMR

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“…Most of the currently available antifungals and antibiotics are unable to cure these biofilm-associated infections effectively (11,12). Therefore, treatment often requires the removal of the infected device, which can be an expensive and painful surgical procedure.…”

mentioning

confidence: 99%

Derivatives of the Mouse Cathelicidin-Related Antimicrobial Peptide (CRAMP) Inhibit Fungal and Bacterial Biofilm Formation

Brucker

Delattin

Robijns

et al. 2014

Antimicrob Agents Chemother

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fWe identified a 26-amino-acid truncated form of the 34-amino-acid cathelicidin-related antimicrobial peptide (CRAMP) in the islets of Langerhans of the murine pancreas. This peptide, P318, shares 67% identity with the LL-37 human antimicrobial peptide. As LL-37 displays antimicrobial and antibiofilm activity, we tested antifungal and antibiofilm activity of P318 against the fungal pathogen Candida albicans. P318 shows biofilm-specific activity as it inhibits C. albicans biofilm formation at 0.15 M without affecting planktonic survival at that concentration. Next, we tested the C. albicans biofilm-inhibitory activity of a series of truncated and alanine-substituted derivatives of P318. Based on the biofilm-inhibitory activity of these derivatives and the length of the peptides, we decided to synthesize the shortened alanine-substituted peptide at position 10 (AS10; KLKKIAQKIKN FFQKLVP). AS10 inhibited C. albicans biofilm formation at 0.22 M and acted synergistically with amphotericin B and caspofungin against mature biofilms. AS10 also inhibited biofilm formation of different bacteria as well as of fungi and bacteria in a mixed biofilm. In addition, AS10 does not affect the viability or functionality of different cell types involved in osseointegration of an implant, pointing to the potential of AS10 for further development as a lead peptide to coat implants.

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“…To this end, E. coli were grown in tryptone broth medium in the presence or absence of amoxicillin as positive control, NSAIDs and Amoxicillin combined with aspirin for 24 h. The bacterial culture was incubated with ethidium bromide for 20 min at room temperature in the dark. Membrane permeability was (Bink et al, 2012).…”

Section: Membrane-permeability Assaymentioning

confidence: 99%

Evaluation of antibacterial activity of some non-steroidal anti-inflammatory drugs against Escherichia coli causing urinary tract infection

Ahmed¹,

El-Baky²,

Ahmed³

et al. 2016

Afr. J. Microbiol. Res.

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Extensive use of antibiotics for urinary tract infections has led to the emergence of drug-resistant microorganisms and one solution to this problem is to search for non-antibiotic compounds that exert anti-bacterial activity through different mechanisms such as non-steroidal anti-inflammatory drugs (NSAIDs). In this study, out of 100 urine samples; 48 Escherichia coli strains were detected, 47.9% were multi-drug resistant. The antibiogram resistance pattern of the strains was carried out by agar dilution method. Diclofenac sodium, indomethacin, aspirin and ibuprofen were tested against the E. coli isolates. Diclofenac sodium showed the lowest MIC 50 and MIC 90 ; 8 and 256 g/ml, respectively. Aspirin showed MIC 50 of 64 g/ml, while both indomethacin and ibuprofen showed MIC 50 of 256 g/ml. Indomethacin, aspirin and ibuprofen showed the same MIC 90 of 1024 g/ml. The combined effects of the four NSAIDs and five antibiotics (Amoxicillin, Augmentin, Cefotaxime, Ciprofloxacin and Gentamicin) were tested on five resistant clinical E. coli strains by checkerboard dilution technique. All the tested NSAIDs significantly reduced the minimum inhibitory concentrations (MICs) of antibiotics against the tested bacteria and fractional inhibitory concentration indices (FICIs) for this combination ranged from 0.03 to 0.5. In this study, leakage of intracellular components suggests that the effect of NSAIDs on E. coli could be the formation of pores in the plasma membrane and scanning electron microscopy (SEM) observations confirmed the damage to the structural integrity of the tested bacteria. In conclusion, NSAIDs showed antibacterial activity against E. coli causing urinary tract infections (UTIs), a combination of them and antibiotics exhibited good synergism and the mechanism of their action was by damaging the bacterial cell membrane.

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The Nonsteroidal Antiinflammatory Drug Diclofenac Potentiates the In Vivo Activity of Caspofungin Against Candida albicans Biofilms

Cited by 62 publications

References 20 publications

Effect of Some Non steroidal Anti-Inflammatory Drugs on Growth, Adherence and Mature Biofilms of <i>Candida spp</i><i>.</i>

Effect of Some Non steroidal Anti-Inflammatory Drugs on Growth, Adherence and Mature Biofilms of <i>Candida spp</i><i>.</i>

Derivatives of the Mouse Cathelicidin-Related Antimicrobial Peptide (CRAMP) Inhibit Fungal and Bacterial Biofilm Formation

Evaluation of antibacterial activity of some non-steroidal anti-inflammatory drugs against Escherichia coli causing urinary tract infection

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