The Nonribosomal Peptide Valinomycin: From Discovery to Bioactivity and Biosynthesis

Huang, San Yuan; Liu, Yushi; Liu, Wanqiu; Neubauer, Peter; Li, Jian

doi:10.3390/microorganisms9040780

Cited by 35 publications

(23 citation statements)

References 133 publications

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“…Finally, mining through the CS090a genome sequence we were only able to find one NRPS type BGC with moderate similarity (56%) to the valinomycin/montanastatin BGC (BGC0001846; [57]) and to the valinomycin BGC (BGC0000453; [58]). In-depth analysis of the described valinomycin gene cluster and a literature revision pointed out that only two genes within the cluster (both encoding NRPSs) were necessary to synthetize the compound and that another thioesterase coding gene improved its production [59,60]. Homologues of these three "core" genes were found within the putative BGC predicted by antiSMASH in the CS090a genome thus we could link this BGC with the valinomycin production in this Streptomycete strain.…”

Section: Correlation Between Bgcs Detected By Antismash Analysis and Dereplication Resultsmentioning

confidence: 86%

A Multidisciplinary Approach to Unraveling the Natural Product Biosynthetic Potential of a Streptomyces Strain Collection Isolated from Leaf-Cutting Ants

et al. 2021

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The rapid emergence of bacterial resistance to antibiotics has urged the need to find novel bioactive compounds against resistant microorganisms. For that purpose, different strategies are being followed, one of them being exploring secondary metabolite production in microorganisms from uncommon sources. In this work, we have analyzed the genome of 12 Streptomyces sp. strains of the CS collection isolated from the surface of leaf-cutting ants of the Attini tribe and compared them to four Streptomyces model species and Pseudonocardia sp. Ae150A_Ps1, which shares the ecological niche with those of the CS collection. We used a combination of phylogenetics, bioinformatics and dereplication analysis to study the biosynthetic potential of our strains. 51.5% of the biosynthetic gene clusters (BGCs) predicted by antiSMASH were unknown and over half of them were strain-specific, making this strain collection an interesting source of putative novel compounds.

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Section: Correlation Between Bgcs Detected By Antismash Analysis and Dereplication Resultsmentioning

confidence: 86%

A Multidisciplinary Approach to Unraveling the Natural Product Biosynthetic Potential of a Streptomyces Strain Collection Isolated from Leaf-Cutting Ants

et al. 2021

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“…and closely related Rothia species as previous reported [55] (Table 2). The presence of the valinomycin clusters in R. nasisuis likely provide this species with a competitive advantage to prevail in the porcine tonsil microbiota and influence the composition of the microbiota in the first weeks of piglet life [7,54]. We identified 18 novel NRPS gene clusters in the genomes of R. nasimurium species using BigScape.…”

Section: Discussionmentioning

confidence: 99%

Comparative genomics of Rothia species reveals diversity in novel biosynthetic gene clusters and ecological adaptation to different eukaryotic hosts and host niches

Oliveira

Baarlen

et al. 2022

Microbial Genomics

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Rothia species are understudied members of the phylum Actinobacteria and prevalent colonizers of the human and animal upper respiratory tract and oral cavity. The oral cavity, including the palatine tonsils, is colonized by a complex microbial community, which compete for resources, actively suppress competitors and influence host physiology. We analysed genomic data from 43 new porcine Rothia isolates, together with 112 publicly available draft genome sequences of Rothia isolates from humans, animals and the environment. In all Rothia genomes, we identified biosynthetic gene clusters predicted to produce antibiotic non-ribosomal peptides, iron scavenging siderophores and other secondary metabolites that modulate microbe–microbe and potentially microbe–host interactions. In vitro overlay inhibition assays corroborated the hypothesis that specific strains produce natural antibiotics. Rothia genomes encode a large number of carbohydrate-active enzymes (CAZy), with varying CAZy activities among the species found in different hosts, host niches and environments. These findings reveal competition mechanisms and metabolic specializations linked to ecological adaptation of Rothia species in different hosts.

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“…They are compounds widely distributed among Streptomyces genus [ 77 ]. Valinomycin is a peptide antibiotic, which was shown to be effective against Candida, Cryptococcus albidus , and plant pathogen Botrytis cinerea [ 78 , 79 , 80 ]. Furthermore, both strains pose BGCs, encoding several desferrioxamine B siderophores (100% similarity).…”

Section: Discussionmentioning

confidence: 99%

Antifungal Activity and Biosynthetic Potential of New Streptomyces sp. MW-W600-10 Strain Isolated from Coal Mine Water

Siupka

Hansen

Schier

et al. 2021

IJMS

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Crop infections by fungi lead to severe losses in food production and pose risks for human health. The increasing resistance of pathogens to fungicides has led to the higher usage of these chemicals, which burdens the environment and highlights the need to find novel natural biocontrol agents. Members of the genus Streptomyces are known to produce a plethora of bioactive compounds. Recently, researchers have turned to extreme and previously unexplored niches in the search for new strains with antimicrobial activities. One such niche are underground coal mine environments. We isolated the new Streptomyces sp. MW-W600-10 strain from coal mine water samples collected at 665 m below ground level. We examined the antifungal activity of the strain against plant pathogens Fusarium culmorum DSM62188 and Nigrospora oryzae roseF7. Furthermore, we analyzed the strain’s biosynthetic potential with the antiSMASH tool. The strain showed inhibitory activity against both fungi strains. Genome mining revealed that it has 39 BGCs, among which 13 did not show similarity to those in databases. Additionally, we examined the activity of the Streptomyces sp. S-2 strain isolated from black soot against F. culmorum DSM62188. These results show that coal-related strains could be a source of novel bioactive compounds. Future studies will elucidate their full biotechnological potential.

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The Nonribosomal Peptide Valinomycin: From Discovery to Bioactivity and Biosynthesis

Cited by 35 publications

References 133 publications

A Multidisciplinary Approach to Unraveling the Natural Product Biosynthetic Potential of a Streptomyces Strain Collection Isolated from Leaf-Cutting Ants

A Multidisciplinary Approach to Unraveling the Natural Product Biosynthetic Potential of a Streptomyces Strain Collection Isolated from Leaf-Cutting Ants

Comparative genomics of Rothia species reveals diversity in novel biosynthetic gene clusters and ecological adaptation to different eukaryotic hosts and host niches

Antifungal Activity and Biosynthetic Potential of New Streptomyces sp. MW-W600-10 Strain Isolated from Coal Mine Water

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