Antifungal Activity and Biosynthetic Potential of New Streptomyces sp. MW-W600-10 Strain Isolated from Coal Mine Water

Abstract

Crop infections by fungi lead to severe losses in food production and pose risks for human health. The increasing resistance of pathogens to fungicides has led to the higher usage of these chemicals, which burdens the environment and highlights the need to find novel natural biocontrol agents. Members of the genus Streptomyces are known to produce a plethora of bioactive compounds. Recently, researchers have turned to extreme and previously unexplored niches in the search for new strains with antimicrobial activities. One such niche are underground coal mine environments. We isolated the new Streptomyces sp. MW-W600-10 strain from coal mine water samples collected at 665 m below ground level. We examined the antifungal activity of the strain against plant pathogens Fusarium culmorum DSM62188 and Nigrospora oryzae roseF7. Furthermore, we analyzed the strain’s biosynthetic potential with the antiSMASH tool. The strain showed inhibitory activity against both fungi strains. Genome mining revealed that it has 39 BGCs, among which 13 did not show similarity to those in databases. Additionally, we examined the activity of the Streptomyces sp. S-2 strain isolated from black soot against F. culmorum DSM62188. These results show that coal-related strains could be a source of novel bioactive compounds. Future studies will elucidate their full biotechnological potential.

Revealing Genome-Based Biosynthetic Potential of Streptomyces sp. BR123 Isolated from Sunflower Rhizosphere with Broad Spectrum Antimicrobial Activity

“…Particularly, the NRPS, NRPS-like, hybrid gene clusters, and majority of the peptide butyrolactone shared resemblance with antibacterial compounds, while most polyketides and other gene clusters shared similarity with anticancer and pigmented compounds. However, low degree of similarity was observed in most cases, suggesting the occurrence of possibly novel biosynthetic gene clusters [ 37 , 38 ].…”

Mining the nanotube-forming Bacillus amyloliquefaciens MR14M3 genome for determining anti-Candida auris and anti-Candida albicans potential by pathogenicity and comparative genomics analysis

“…This discovery denotes that the goal of the current study achieved with anti- C. auris metabolites [62] , [67] . Earlier studies have highlighted the use of bacterial genome mining for identifying metabolic potential against Candida albicans but no promising studies against C. auris [29] , [31] , [32] , [33] , [34] , [35] . The native bacterial strain from the present study, B. amyloliquefaciens MR14M3, was identified with biosynthesis-related gene clusters of possible anti- Candida auris and anti- Candida albicans potential natural compounds that were in line with the B. amyloliquefaciens DSM 7 genome [68] and Bacillus amyloliquefaciens FZB42 [69] .…”

“…Long-read Nanopore sequencing was chosen over other short-read high-throughput sequencing methods because they offer insufficient phylogenetic resolution and have a low level of integrity for the majority of the assembled area [79] . The MR14M3 genome was a single contig, achieved using long-read sequencing with a high portion in N50, which led us to predict many BGCs with the highest similarity [32] . The current study had great coverage in the genome, allowing us to produce high-quality genomes where the BGCs did not span a large number of contigs because of a single contig, which ensured the accuracy of prediction of antiSMASH [49] .…”

“…strain S-2 isolated from black soot contained 39 biosynthesis-related gene clusters (BGCs) with anti- Fusarium culmorum activity based on genome mining [32] . The value of genome mining for identifying biosynthetic potential in bacterial isolates has been highlighted in several studies [32] , [33] , [34] , [35] . Nevertheless, studies on bacterial genome mining in potential isolates with anti- C. auris activity is rare.…”

Streptomyces: The biofactory of secondary metabolites

“…The resurgence of fungal infection is a public health crisis. Secondary metabolites produced by Actinobacteria, particularly members of the Streptomyces genus, are known to have a wide range of biological activities, including antifungal compounds ( Siupka et al, 2021 ).…”