Streptomyces: The biofactory of secondary metabolites

Alam, Khorshed; Mazumder, Arpita; Sikdar, Suranjana; Zhao, Yiming; Hao, Jinfang; Song, Chaoyi; Wang, Yanyan; Sarkar, Rajib; Islam, Shofiq; Zhang, Youming; Li, Aiying

doi:10.3389/fmicb.2022.968053

Cited by 113 publications

(81 citation statements)

References 156 publications

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“…Among these 19 shared BGCs, some were previously reported to encode products conserved across the Streptomyces genus. These include https://www.wikidata.org/wiki/Q414414, which prevents osmotic stress, https://www.wikidata.org/wiki/Q420233, responsible for the characteristic odor of moist soil, https://www.wikidata.org/wiki/Q14124950, which plays a role in prokaryotic membrane stability, spore pigments, as well as the siderophore https://www.wikidata.org/wiki/Q419618 (Alam et al, 2022; Ward & Allenby, 2018). Additionally, the three genomes contained other BGCs encoding various antibiotics, such as https://www.wikidata.org/wiki/Q27122616 (Zhao et al, 2008), https://www.wikidata.org/wiki/Q5031709 (Chen et al, 2003), calcium dependant‐antibiotics (Hojati et al, 2002), and https://www.wikidata.org/wiki/Q106715135 (Liu et al, 2017).…”

Section: Resultsmentioning

confidence: 99%

“…Among these 19 shared BGCs, some were previously reported to encode products conserved across the Streptomyces genus. These include ectoine, which prevents osmotic stress, geosmin, responsible for the characteristic odor of moist soil, hopene, which plays a role in prokaryotic membrane stability, spore pigments, as well as the siderophore desferrioxamine (Alam et al, 2022;Ward & Allenby, 2018).…”

Section: Theoretical Spectral Libraries Searchmentioning

confidence: 99%

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Comparative genomic and metabolomic study of three Streptomyces sp. differing in biological activity

Gillon,

Abdelrahman,

Abou‐Mansour

et al. 2023

MicrobiologyOpen

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The Streptomyces genus is known to produce many specialized metabolites of value for medicine, but the potential of these metabolites in agronomy remains largely unexplored. In this study, we investigated three phylogenetically closely related Streptomyces strains (B5, B91, and B135) isolated from three distinct soil samples in Sudan. Despite belonging to the same species, these strains exhibited different ranges of Phytophthora infestans inhibition. The objective of this work was to identify the active compound(s) responsible for the inhibition of P. infestans and of other plant pathogens by comparing the genomes and metabolomes of the three strains which showed distinct activity patterns: B5 was the strongest inhibitor of oomycetes, B5 and B91 both inhibited most fungi and B135 was the only strain showing antibacterial activity. Our comparative genomic and metabolomic analysis identified borrelidin as the bioactive compound underlying B5's strong anti‐oomycete activity and highlighted a few other metabolites as putative candidates underlying the strains' antifungal and antibacterial activities. This study illustrates the power of comparative genomics and metabolomics on phylogenetically closely related strains of differing activities to highlight bioactive compounds that could contribute to new sustainable crop protection strategies.

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Section: Resultsmentioning

confidence: 99%

Section: Theoretical Spectral Libraries Searchmentioning

confidence: 99%

Comparative genomic and metabolomic study of three Streptomyces sp. differing in biological activity

Gillon,

Abdelrahman,

Abou‐Mansour

et al. 2023

MicrobiologyOpen

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“…Gram-positive, filamentous soil bacteria of the Streptomyces genus are of great pharmaceutical and economic importance, since they produce a huge variety of bioactive molecules useful to human health or agriculture such as antibiotics, anti-cancer drugs, pesticides and herbicides ( Pham et al, 2019 ; Barka et al, 2016 ; Alam et al, 2022 ). The biosynthesis of these molecules is usually triggered in condition of phosphate limitation ( Martin, 2004 ) that is correlated with a low intracellular ATP content ( Esnault et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Metabolic adjustments in response to ATP spilling by the small DX protein in a Streptomyces strain

Apel

Levasseur²,

Lejeune³

et al. 2023

Front. Cell Dev. Biol.

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ATP wasting is recognized as an efficient strategy to enhance metabolic activity and productivity of specific metabolites in several microorganisms. However, such strategy has been rarely implemented in Streptomyces species whereas antibiotic production by members of this genus is known to be triggered in condition of phosphate limitation that is correlated with a low ATP content. In consequence, to assess the effects of ATP spilling on the primary and specialized metabolisms of Streptomyces, the gene encoding the small synthetic protein DX, that has high affinity for ATP and dephosphorylates ATP into ADP, was cloned in the integrative vector pOSV10 under the control of the strong ErmE promoter. This construct and the empty vector were introduced into the species Streptomyces albogriseolus/viridodiastaticus yielding A37 and A36, respectively. A37 yielded higher biomass than A36 indicating that the DX-mediated ATP degradation resulted into a stimulation of A37 metabolism, consistently with what was reported in other microorganisms. The comparative analysis of the metabolomes of A36 and A37 revealed that A37 had a lower content in glycolytic and Tricarboxylic Acid Cycle intermediates as well as in amino acids than A36, these metabolites being consumed for biomass generation in A37. In contrast, the abundance of other molecules indicative either of energetic stress (ADP, AMP, UMP, ornithine and thymine), of activation (NAD and threonic acid) or inhibition (citramalic acid, fatty acids, TAG and L-alanine) of the oxidative metabolism, was higher in A37 than in A36. Furthermore, hydroxyl-pyrimidine derivatives and polycyclic aromatic polyketide antibiotics belonging to the angucycline class and thought to have a negative impact on respiration were also more abundantly produced by A37 than by A36. This comparative analysis thus revealed the occurrence in A37 of antagonistic metabolic strategies, namely, activation or slowing down of oxidative metabolism and respiration, to maintain the cellular energetic balance. This study thus demonstrated that DX constitutes an efficient biotechnological tool to enhance the expression of the specialized metabolic pathways present in the Streptomyces genomes that may include cryptic pathways. Its use thus might lead to the discovery of novel bioactive molecules potentially useful to human health.

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“…Actinobacteria play a profound role in the marine environment by contributing to the breakdown and recycling of organic compounds [2]. They are gaining importance not only for their taxonomic and ecological perspectives, but also for their unique secondary metabolites notably antibiotics [3,4,5] and enzymes [6]. Despite being such a potential source of bioactive compounds, the probiotic role of actinomycetes mainly in aquaculture systems is comparatively less explored.…”

Section: Introductionmentioning

confidence: 99%

Marine actinomycete Streptomyces variabilis S26 as biocontrol agent for vibriosis in shrimp larval rearing systems

Solomon

Babu

Kachiprath

et al. 2023

Preprint

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Indiscriminate use of antibiotics has led to the emergence of antibiotic resistant microbes and the loss of natural flora in aquaculture systems ultimately necessitating the ban of many of the chemotherapeutants in aquaculture. Actinobacteria play a profound role in the biogeochemical cycling in the marine environment and they represent the principal source of secondary metabolites with antimicrobial property. In the present study, 98 marine derived actinomycete isolates were screened for antimicrobial activity against the common aquatic pathogens. A potent actinomycete isolate S26, identified as Streptomyces variabilis based on 16S rRNA gene sequencing was used for further study. Optimization of the fermentation medium for secondary metabolite production was carried out by response surface methodology (RSM) using DESIGN EXPERT. The ANOVA of the quadratic regression model demonstrated that the model was highly significant for the response concerned i.e., antimicrobial activity as evident from the Fisher’s F- test with a very low probability value [(P model>F) = 0.0001]. Of the 10 different solutions suggested by the software, the most suitable composition was found to be starch, 1.38 %; soy powder, 0.88 %; ammonium sulphate, 0.16 % and salinity, 27.76 ‰. S. variabilis S26 cultured in the optimized production medium was applied in the Penaeus monodon larval rearing system and the total Vibrio count and survival rate were estimated. S. variabilis S26 treatment showed a significant reduction in Vibrios and better survival in the Penaeus monodon culture system compared to the control.

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Streptomyces: The biofactory of secondary metabolites

Cited by 113 publications

References 156 publications

Comparative genomic and metabolomic study of three Streptomyces sp. differing in biological activity

Comparative genomic and metabolomic study of three Streptomyces sp. differing in biological activity

Metabolic adjustments in response to ATP spilling by the small DX protein in a Streptomyces strain

Marine actinomycete Streptomyces variabilis S26 as biocontrol agent for vibriosis in shrimp larval rearing systems

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