The non-specific esterases of mouse lung

Abstract

The non-specific esterases of the lung of the house mouse, M. musculus, were examined by polyacrylamide electrophoresis and by isoelectric focusing. At least 13 different esterases were distinguished and identified, mainly by their catalytic properties, susceptibility to inhibition, developmental patterns and phenotypic variation amongst different strains. A list of diagnostic features of the 13 esterases was presented. None of the esterases was lung-specific. However, the pattern of esterases found in the adult lung was characteristic of that organ. It was pointed out that this pattern is associated with the high degree of tissue differentiation in the adult lung. At least 8 esterases were found which belong to the isozyme system of carboxylesterase EC 3.1.1.1, under the control of genes located on chromosome 8. These esterases accounted for about 90% of the esterase activity in the lung.

A Discrete Subpopulation of Dendritic Cells Transports Apoptotic Intestinal Epithelial Cells to T Cell Areas of Mesenteric Lymph Nodes

“…1 G). NSE exists as different isoforms with different electrophoretic mobilities (30,33,34). To identify the origin of the NSE in OX41 Ϫ L-DCs, we prepared NSE zymograms from IECs, L-DCs, and other cells and tissues (Fig.…”

“…In macrophages and other DCs (including OX41 ϩ DCs), NSE is diffuse and perinuclear, but brief incubation of OX41 Ϫ L-DCs with substrate shows NSE in large cytoplasmic inclusions. Positive identification of NSE origins is possible, because NSE represents different enzymes existing in different isoforms (30,33,38,39) that can be distinguished by their electrophoretic mobility. Expression patterns of variants differ in different cells and tissues.…”

“…For simultaneous detection of NSE reactivity and Ab binding, cells or sections were fixed with 2% paraformaldehyde, reacted for NSE, washed extensively, and then labeled with Abs as above. To identify NSE variants, zymograms were prepared according to von Deimling et al (30). In brief, cells or tissues were lysed in lysis buffer containing 0.1% Triton X-100 and 15 mM EDTA and sonicated on ice for 10-30 s using an MSE sonicator.…”

Dexamethasone palmitate large porous particles: A controlled release formulation for lung delivery of corticosteroids

“…Experimental concentrations could be explained by three main phenomena as described in Figure 8: powder solubilization, DXP hydrolysis into DXM, DXP/DXM distribution in lung tissue and DXP/DXM passage in the blood stream. Indeed, the first part of the DXP curve in ELF may correspond to the solubilization of particles to release DXP molecules along with hydrolysis of DXP into DXM occuring due to the presence of esterases in the ELF (Basu et al, 1988;Deimling et al, 1983). The fast decrease of DXP concentration in ELF probably corresponds to DXP distribution in lung tissue.…”