The NF-kB and Collagen Type 1 Expression in Dental Pulp after Treated Calcium Hydroxide Combined with Propolis

Abstract

Objective To analyze the expression of nuclear factor kappa B (NF-kB) and collagen type 1 on dental pulp after a treated combination of calcium hydroxide and propolis. Materials and Methods The first maxillary molars of 30 rats were mechanically perforated. Teeth were divided into three groups of 10 for two separate extraction time frames, giving a total of 60 rats. The control groups were treated with Cention, the second treatment groups were treated with calcium hydroxide, and the third treatment groups were treated with a combination of calcium hydroxide and propolis. Final restoration was done with Cention. The teeth were extracted on days 7 and 14, and the expression of NF-kB and collagen type I was analyzed using immunohistochemistry. Results There is lowest NF-kB expression and highest collagen type 1 expression on dental pulp after treated with a combination of calcium hydroxide and propolis on days 7 and 14 (p < 0.05). Conclusion The combination of calcium hydroxide and propolis inhibits pulp inflammation and stimulates regeneration through decreasing the NF-kB expression and increasing collagen type 1.

Long-Term Assessment of Contemporary Ion-Releasing Restorative Dental Materials

“…While mechanical properties of resin composites [18,29,30], glass-ionomers [14,15], and giomers [19,21] are sufficiently explored, studies focused on alkasite materials are scarce and mainly investigate the powder-liquid hand-mixed Cention N [31][32][33][34]. Besides the work of Par and co-workers [4,22,24,25] that focused on ion-releasing properties of Cention, a PubMed search of articles including the capsulated version of Cention resulted in finding only three papers studying fluoride release [35], wear behavior [36], or biologic effects on pulp cells [37]. The data about the long-term mechanical behavior of capsulated alkasite Cention used in either self-cure or light-cure mode is still lacking, especially considering the compositional modifications of the capsulated version in contrast to the predecessor Cention N.…”

The Effect of Lipoteichoic Acid from Lactobacillus plantarum on Dental Pulp Inflammation

“…The dental pulp inflammation was created in the left upper molar by opening the pulp chamber using a round bur and high-speed rotary instrument. 11 LTA-Lp was administered at a dose of 10 and 15 µg/mL as much as 1 µL of micropipette using a sterile paper point on the surface of the perforated pulp chamber; this was repeated five times. The cavity was then cleaned with a cotton pellet, filled with a temporary filling (Cention-N, Ivoclar Vivadent, Liechtenstein), and then irradiated with a light cure technique for 40 seconds.…”

The Role of microRNAs in Pulp Inflammation

“…MiR-150 has as potential target genes interleukin (IL)-6, nuclear factor (κB)-1, Janus kinase (JAK)-2, and interleukin 1 receptor-associated kinase (IRAK)-2 [51]. During pulp inflammation, studies have reported a significant increase of IL-6 [69] at the protein [70][71][72] and mRNA [73,74] level, as well as phosphorylation of IRAK1 [75], expression of NF-κB [76], and activation of the JAK pathway [77]. The production of these pro-inflammatory mediators can be correlated with the degree of inflammation in the pulp tissue, in which miR-150 seems to have an important role as a regulator of inflammatory responses [111], since its overexpression has been shown to have a protective effect on inflammatory responses [112,113].…”