The new system of shorter porcine oocyte in vitro maturation (18 hours) using ≥8 mm follicles derived from cumulus-oocyte complexes

Kwak, Seong-Sung; Yoon, Jong Hwan; Cheong, Seung-A; Jeon, Yubyeol; Lee, Eunsong; Hyun, Sang‐Hwan

doi:10.1016/j.theriogenology.2013.09.028

Cited by 20 publications

(14 citation statements)

References 46 publications

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“…Improved development of in vitro matured oocytes can only be achieved by expanding our knowledge regarding the complex dialog between the oocyte and its surrounding somatic cells within follicle [4,5]. Nevertheless, this has proven to be challenging because: 1) dealing with a heterogeneous population of oocytes harvested from follicles of different size (2-6 mm in sheep and goat, 2-8 mm in bovine and < 12mm in human) is a formidable issue; 2) cumulus oocyte complexes (COCs) derived from small antral follicles possess less competence in response to regulatory and specific ligands because of an immature signaling capacity [6][7][8][9][10]; and 3) Isolation of COCs from their natural follicular environment results in spontaneous meiotic progression and, thus, asynchronization of nuclear and cytoplasmic maturation [11].…”

Section: Introductionmentioning

confidence: 99%

Granulosa secreted factors improve the developmental competence of cumulus oocyte complexes from small antral follicles in sheep

et al. 2020

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Oocyte in vitro maturation can be improved by mimicking the intra-follicular environment. Oocyte, cumulus cells, granulosa cells, and circulating factors act as meiotic regulators in follicles and maintain oocyte in the meiotic phase until oocyte becomes competent and ready to be ovulated. In a randomized experimental design, an ovine model was used to optimize the standard in vitro maturation media by Granulosa secreted factors. At first, the development capacity of oocyte derived from medium (>4 to 6 mm) and small (2 to �4 mm) size follicles was determined. Differential gene expression of granulosa secreted factors and their receptors were compared between the cumulus cells of the two groups. Then, the best time and concentration for arresting oocytes at the germinal vesicle stage by natriuretic peptide type C (CNP) were determined by nuclear staining in both groups. Oocyte quality was further confirmed by calcein uptake and gene expression. The developmental competence of cumulus oocyte complexes derived from small size follicles that were cultured in the presence of CNP in combination with amphiregulin (AREG) and prostaglandin E2 (PGE2) for 24 h was determined. Finally, embryo quality was specified by assessing expressions of NANOG, SOX2, CDX2, OCT4, and TET1. The cumulus oocyte complexes derived from small size follicles had a lower capacity to form blastocyst in comparison with cumulus oocyte complexes derived from medium size follicles. Prostaglandin E receptor 2 and prostaglandin-endoperoxide synthase 2 had significantly lower expression in cumulus cells derived from small size follicles in comparison with cumulus cells derived from medium size follicles. Natriuretic peptide type C increased the percentage of cumulus oocyte complexes arresting at the germinal vesicle stage in both oocytes derived from medium and small follicles. Gap junction communication was also improved in the presence of natriuretic peptide type C. In oocytes derived from small size follicles; best blastocyst rates were achieved by sequential exposure of cumulus oocyte complexes in [TCM+CNP (6 h), then cultured in TCM+AREG+PGE2 (18h)] and [TCM+CNP (6 h), then cultured in conventional IVM supple-ments+AREG+PGE2 (18h)]. Increased SOX2 expression was observed in [TCM+CNP (6 h), then cultured in TCM+AREG+PGE2 (18h)], while decreased OCT4 expression was

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Section: Introductionmentioning

confidence: 99%

Granulosa secreted factors improve the developmental competence of cumulus oocyte complexes from small antral follicles in sheep

et al. 2020

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“…To date, porcine in vitro embryo production and cryopreservation present poor success compared to mouse and bovine [41], and the efficiency of porcine in vitro maturation remains low. Its improvement may positively influence biomedical technologies, such as the production of transgenic pigs [42]. …”

Section: Introductionmentioning

confidence: 99%

Lipid characterization of individual porcine oocytes by dual mode DESI-MS and data fusion

Pirro

Oliveri

Ferreira

et al. 2014

Analytica Chimica Acta

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The development of sensitive measurements to analyze individual cells is of relevance to elucidate specialized roles or metabolic functions of each cell under physiological and pathological conditions. Lipids play multiple and critical roles in cellular functions and the application of analytical methods in the lipidomics area is of increasing interest. In this work, in vitro maturation of porcine oocytes was studied. Two independent sources of chemical information (represented by mass spectra in the positive and negative ion modes) from single oocytes (immature oocytes, 24-h and 44-h in vitro matured oocytes) were acquired by using desorption electrospray ionization-mass spectrometry (DESI-MS). Low and mid-level data fusion strategies are presented with the aim of better exploring the large amount of chemical information contained in the two mass spectrometric lipid profiles. Data were explored by principal component analysis (PCA) within the two multi-block approaches to include information on free fatty acids, phospholipids, cholesterol-related molecules, di- and triacylglycerols. After data fusion, clearer differences among immature and in vitro matured porcine oocytes were observed, which provide novel information regarding lipid metabolism throughout oocyte maturation. In particular, changes in TAG composition, as well as increase in fatty acid metabolism and membrane complexity were evidenced during the in vitro maturation process. This information can assist the improvement of in vitro embryo production for porcine species.

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“…Oocyte developmental competence, which is acquired gradually during oocyte growth and maturation in the ovarian follicle, is regulated by a number of signals molecules as well as cumulus cells 41 – 43 . Furthermore, previous studies have shown that COCs derived from SFs lack the ability to respond to regulatory and specific ligands because of an immature signaling capacity 44 – 46 . Thus, we hypothesized that during CNP pre-IVM period, the signaling networks in oocytes and surrounding cumulus cells from SFs, which participate in the development of oocyte competence, might be insufficient for promoting oocyte cytoplasmic maturation compared to those in oocytes and surrounding cumulus cells from MFs.…”

Section: Discussionmentioning

confidence: 99%

Effects of C-type natriuretic peptide on meiotic arrest and developmental competence of bovine oocyte derived from small and medium follicles

Jia

Wang

2020

Sci Rep

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The present study aimed to evaluate the effects of C-type natriuretic peptide (CNP) on meiotic arrest and developmental competence of bovine oocyte derived from follicles of different sizes. Collected immature cumulus-oocyte complexes from small follicles (< 3 mm) and medium follicles (3–8 mm) were cultured for 6 h in basal medium supplementated without or with 200 nM CNP. We observed that CNP effectively sustained meiotic arrest at germinal vesicle stage in in vitro cultured bovine oocytes from follicles of different sizes. Moreover, CNP treatment significantly improved the levels of cGMP in both cumulus cells and oocytes, as well as the levels of cAMP in oocytes regardless of follicle size. Based on the above results, we tested the effect of a novel in vitro maturation (IVM) system based on CNP-pretreatment, including a pre-IVM phase for 6 h using 200 nM CNP, followed by a extended IVM phase for 28 h, on developmental competence of bovine oocyte derived from small follicles (< 3 mm) and medium follicles (3–8 mm) compared to standard IVM system. The results showed that athough the novel IVM system based on CNP-pretreatment enhanced the developmental potencial of oocytes obtained from large follicles, but had no effect on the developmental comptence of oocytes obtained from small follicles.

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The new system of shorter porcine oocyte in vitro maturation (18 hours) using ≥8 mm follicles derived from cumulus-oocyte complexes

Cited by 20 publications

References 46 publications

Granulosa secreted factors improve the developmental competence of cumulus oocyte complexes from small antral follicles in sheep

Granulosa secreted factors improve the developmental competence of cumulus oocyte complexes from small antral follicles in sheep

Lipid characterization of individual porcine oocytes by dual mode DESI-MS and data fusion

Effects of C-type natriuretic peptide on meiotic arrest and developmental competence of bovine oocyte derived from small and medium follicles

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