Effects of C-type natriuretic peptide on meiotic arrest and developmental competence of bovine oocyte derived from small and medium follicles

Jia, Zhenwei; Wang, Xueli

doi:10.1038/s41598-020-75354-5

Cited by 10 publications

(6 citation statements)

References 47 publications

(55 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, it has been identified that inhibition of NPR2 activity using Gö6976, a relatively specific inhibitor, can effectively abrogates CNP-stimulated CREB phosphorylation in bovine oocytes. In addition, we previously found that inhibition of NPR2 activity using Gö6976, prevented CNP-induced elevation in cGMP and cAMP levels in bovine oocyte 31 . These results revealed that CNP can activate CREB via the maintenance of higher levels of cGMP/cAMP in bovine oocyte, which is similar to those foundation in animal somatic cells by modulating effects of cGMP/cAMP on CREB activity 25 , 26 .…”

Section: Discussionmentioning

confidence: 92%

Pre-IVM with C-type natriuretic peptide promotes mitochondrial biogenesis of bovine oocytes via activation of CREB

Zhang,

Jia

2024

Sci Rep

View full text Add to dashboard Cite

The aim of this study was to evaluate the effects of C-type natriuretic peptide (CNP) treatment prior to in vitro maturation (IVM) on mitochondria biogenesis in bovine oocyte matured in vitro and explore the related causes. The results showed that treatment with CNP before IVM significantly improved mitochondrial content, elevated the expression of genes related to mitochondria biogenesis, and increased the protein levels of phosphorylation of cAMP-response element binding protein (p-CREB) in bovine oocytes following IVM. However, further studies revealed that treatment with CNP before IVM could not increased the protein levels of p-CREB in bovine oocytes when natriuretic peptide receptor 2 activities was inhibited using the relative specific inhibitor Gö6976. In addition, treatment with CNP before IVM could not improved mitochondrial content or elevated the expression of genes related to mitochondria biogenesis in bovine oocytes when CREB activities was abolished using the specific inhibitor 666–15. In summary, these results provide evidence that treatment of bovine oocytes with CNP before IVM promotes mitochondrial biogenesis in vitro, possibly by activating CREB.

show abstract

Section: Discussionmentioning

confidence: 92%

Pre-IVM with C-type natriuretic peptide promotes mitochondrial biogenesis of bovine oocytes via activation of CREB

Zhang,

Jia

2024

Sci Rep

View full text Add to dashboard Cite

show abstract

“…CNP is a peptide consisting of 22 amino acids that are widely distributed in the central nervous system, endothelium, and gastrointestinal and genitourinary tracts [19,20]. In recent years, the effects of CNP on mammalian folliculogenesis, oocytes, and embryonic development have been reported [6,21,22]. Thus, we delved into the mechanisms by which CNP improved the developmental potential of goat oocytes.…”

Section: Discussionmentioning

confidence: 99%

“…Mitochondria distribution and activity are essential for oocyte maturation, fertilization, and embryonic development [6]. First, we evaluated MII oocytes' mitochondrial membrane potential (MMP) using JC-1 staining.…”

Section: Cnp Treatment Before Ivm Improved Oocytes' Mitochondrial Mem...mentioning

confidence: 99%

“…cGMP is transferred into the oocyte via transzonal projections (TZPs) and gap junctions (Gj) to inhibit phosphodiesterase 3A (PDE3A) activity, causing cyclic adenosine monophosphate (cAMP) degradation to slow down and, in turn, maintaining a high cAMP level and meiotic arrest [3,4]. Several species have demonstrated an enhancement in oocyte maturation quality and blastocyst development through the implementation of CNP pre-treatment prior to IVM [5][6][7][8].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

C-Type Natriuretic Peptide Pre-Treatment Improves Maturation Rate of Goat Oocytes by Maintaining Transzonal Projections, Spindle Morphology, and Mitochondrial Function

Xu,

Pan,

Yin

et al. 2023

Animals

View full text Add to dashboard Cite

C-type natriuretic peptide (CNP) is a peptide molecule naturally found in follicles and can be used to extend meiotic resumption and enhance the potential for oocytes to develop. However, the mechanism by which CNP improves goat oocyte quality remains unclear. In this study, cumulus–oocyte complexes (COCs) from goats were pre-treated with CNP prior to IVM, and the results showed that pre-treatment with CNP enhanced goat oocyte maturation. First, we discovered that CNP maintained communication between cumulus cells and oocytes by regulating the transzonal projections (TZPs). We then found that CNP treatment reduced abnormal spindle formation and increased the expression of genes associated with spindle assembly and the spindle assembly checkpoint. Moreover, further analysis showed that oocytes exhibited better antioxidant ability in the CNP treatment group, which mainly manifested in higher glutathione (GSH) and lower reactive oxygen species (ROS) concentrations. Enhanced mitochondrial activity was signified via the augmented expression of mitochondrial oxidative metabolism and fusion and fission-related genes, thus diminishing the apoptosis of the oocytes. Overall, these results provide novel insights into the potential mechanism by which CNP treatment before IVM can improve oocyte quality.

show abstract

“…Thus, the transfer of cGMP from CCs to oocytes via gap junctions is critical in the inhibition of PDE3A actions to hydrolyze cAMP. C-type natriuretic peptide, expressed in high levels in mural granulosa cells, was found to act on its receptor, natriuretic peptide receptor 2, in CCs to generate cGMP (43)(44)(45). The studies collectively suggest that as LH triggers the closure of gap junctions (46), the CC to oocyte transfer of cGMP would be reduced and there would be an increased level of PDE3A activity, ultimately leading to meiotic resumption.…”

Section: To Oocyte Signals and Regulationmentioning

confidence: 98%

Identifying fertilization-ready metaphase II stage oocytes beyond the microscope: a proposed molecular path forward

Sun

Yeh

2021

F&S Reviews

View full text Add to dashboard Cite

One key variable in the success of in vitro fertilization (IVF) cycles is the maturation of oocytes for fertilization. Current practices of both conventional IVF and intracytoplasmic sperm injection do not evaluate the maturity of eggs immediately at the time of oocyte retrieval. In conventional IVF, all retrieved eggs are inseminated with sperm without further evaluation of their maturity. With conventional IVF, immature oocytes are not identified and are inseminated with resulting poor outcomes. In intracytoplasmic sperm injection, retrieved eggs are denuded of cumulus cells (CCs) for evaluation of maturity before insemination. Immature oocytes are identified after denudation and may not reach their full maturation potential because of the loss of surrounding CCs. Both approaches can benefit from an early noninvasive evaluation of oocyte maturity. Cumulus cells are physically and biochemically connected to oocytes and could serve as a window to look into oocyte maturity. This study reviews current literature on processes essential in oocyte maturation involving CCs as well as messenger RNA (mRNA), microRNA (miRNA), and protein biomarkers of metaphase II (MII) stage oocytes identified in CCs. We use this information as a starting point to propose a path forward in the molecular analysis of CCs as a tool of MII oocyte selection. With future studies proposed in this review, we envision a clinically useful approach of selected mRNA, miRNA, and protein marker analysis and eventually a transition to mRNA-miRNA-protein expression correlation analysis and pathway analysis of CC markers to identify MII oocytes at the time of retrieval and enable metaphase I and germinal vesicle oocytes to be further matured in vitro with intact supporting CCs. (Fertil Steril Rev Ò 2021;2:302-16. Ó2021 by American Society for Reproductive Medicine.

show abstract

Effects of C-type natriuretic peptide on meiotic arrest and developmental competence of bovine oocyte derived from small and medium follicles

Cited by 10 publications

References 47 publications

Pre-IVM with C-type natriuretic peptide promotes mitochondrial biogenesis of bovine oocytes via activation of CREB

Pre-IVM with C-type natriuretic peptide promotes mitochondrial biogenesis of bovine oocytes via activation of CREB

C-Type Natriuretic Peptide Pre-Treatment Improves Maturation Rate of Goat Oocytes by Maintaining Transzonal Projections, Spindle Morphology, and Mitochondrial Function

Identifying fertilization-ready metaphase II stage oocytes beyond the microscope: a proposed molecular path forward

Contact Info

Product

Resources

About