The New Generation of Platelet Additive Solution for Storage at 22°C: Development and Current Experience

Ringwald, Juergen; Zimmermann, Robert; Eckstein, Reinhold

doi:10.1016/j.tmrv.2005.11.003

Cited by 74 publications

(68 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Magnesium and potassium are present in platelet additive solution. These electrolytes inhibit platelet activation and aggregation, although how they work is unclear [10]. Potassium plays an important role in maintaining the platelet membrane potential, [19] and, when absent, potassium will leak rapidly from the platelet and needs to be recovered by energy requiring potassium pumps.…”

Section: Discussionmentioning

confidence: 99%

“…Platelet additive solution replaces 7080% of the plasma in the original platelet unit, these formulations are predicted to have additional benefits: reduced allergic and transfusion reactions [9], decreased transfusion of unwanted antibodies (e.g. ABO, HLA) and increased plasma made for fractionation [10]. Various laboratory tests have been recommended to study platelet storage lesion ranging from most simple test such as pH to more complex tests of platelet function.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Morphological and functional changes in random donor platelets stored for seven days in platelet additive solution

Chandra¹,

Gupta

Kumar

et al. 2011

Int J Blood Transfus Immunohematol

View full text Add to dashboard Cite

Introduction: Platelets are small, anucleated cytoplasmic fragments that play an essential role in blood clotting and wound healing. Platelets are routinely stored in plasma for five days at 22 0 C. Aim: Our objective was to assess platelet morphology and function in random donor platelet stored for seven days in platelet additive solution. Methods: The random donor platelet was prepared by PRP method. Platelet concentrates from 50 blood donor were stored in 100% plasma (n=24) and 20:80 (n=24) additive solution. The RDP were placed in incubator and agitator at 22°C. Results: Out of total 50 samples, 48 samples were analyzed and two were discarded due to the bacterial contamination. On comparing the mean values of platelet count and MPV in both the groups with and without platelet additive solution, no significant difference was observed on day seven of storage period. A significant difference in PDW was observed on day seven (p < 0.001) in

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Morphological and functional changes in random donor platelets stored for seven days in platelet additive solution

Chandra¹,

Gupta

Kumar

et al. 2011

Int J Blood Transfus Immunohematol

View full text Add to dashboard Cite

show abstract

“…Because additive solutions replace 70-80% of the plasma in the original platelet unit, these formulations are predicted to have additional benefits: reduced allergic and febrile transfusion reactions, 33 decreased transfusion of unwanted antibodies (e.g., ABO, HLA) and increased plasma made available for fractionation. 34 Additive solutions could afford technical benefits for pathogen reduction methods as well (PAS-III, or "Inactisol" was created for the EuroSPRITE pathogen reduction study).…”

Section: Additive Solutionsmentioning

confidence: 99%

“…These electrolytes inhibit platelet activation and aggregation, although how they work is unclear. 34 Van der Meer and colleagues 35 compared the in vitro storage characteristics of pooled buffy coat platelets stored for up to 12 days in 100% plasma, or in mixtures of plasma with PAS-II, PAS-III, PAS-IIIM and Composol. They observed that several in vitro markers of platelet quality (pH ≥ 6.8, glucose consumption, lactate production) were reasonably well preserved for 9-12 days in platelets stored either in 100% plasma, or in PAS-IIIM (30% plasma) or Composol (35% plasma).…”

Section: Additive Solutionsmentioning

confidence: 99%

Platelets: Testing, Dosing and the Storage Lesion—Recent Advances

Kaufman¹

2006

Hematology

View full text Add to dashboard Cite

The demand for platelet transfusions continues to grow. Several complementary approaches that may help meet this demand in the future are reviewed. First, platelet bacterial testing is beginning to allow the extension of platelet storage beyond 5 days. Studies are also underway aimed at better preserving viability and function during ex vivo platelet storage: additive solutions and other approaches are being developed to try to negate the "platelet storage lesion." Finally, new approaches to dosing platelets may help extend the limited supply.

show abstract

“…It may reduce the incidence of allergic transfusion reactions, facilitate ABO-incompatible platelet transfusions, enable pathogen inactivation, and make plasma available for other purposes (e.g. for fractionation into blood derivatives) [1]. It is however important to ensure that platelets stored in a platelet additive solution maintain adequate function.…”

Section: Introductionmentioning

confidence: 99%

Investigating the effect of a platelet additive solution on apheresis platelet and fibrin network ultrastructure

Pretorius

Crookes

Oberholzer

et al. 2010

Transfusion and Apheresis Science

View full text Add to dashboard Cite

a b s t r a c tIn thrombotic events and diseases such as cancer, HIV/AIDS, dysfibrinogenaemia, as well as acute incidents (e.g. burn wounds), ultrastructure of platelets and fibrin networks change. In the current study, we compare the ultrastructure of platelets and fibrin networks of apheresis platelets stored in citrated human plasma (CP) and in a first-generation platelet additive solution (PAS) (T-Sol), to that of fresh donor plasma (FP). Eighteen apheresis platelet donors donated platelets on Trima Ò -Accel™ V5.2 and V5.1 cell separators. Six collections were stored for five days in autologous citrated plasma (CP); six collections were stored in 40% citrated human plasma and 60% PAS solution (CP/PAS) controlled, for the duration of storage, at a constant temperature (22 ± 2°C) with continuous flat-bed agitation; and six collections were stored in conditions uncontrolled for temperature and without continuous agitation. On days 1, 3 and 5, equal volumes of human thrombin were mixed with platelets collected in either CP or CP/PAS to form a coagulum (fibrin network containing platelet aggregates), followed by preparation for scanning electron microscopy. Results were compared with platelets and fibrin networks in FP. Typically, in FP, platelet aggregates with smooth membranes and pseudopodia are seen and fibrin networks arrange to form major, thick fibers and scattered, minor, thin fibers. On day 1, in CP and in all CP/PAS units, platelet ultrastructure compared well to that of FP, although the fibrin fibers were denser, with the minor fibers forming a matted layer over the major fibers. On day 3, in platelet units uncontrolled for temperature and without continuous agitation during storage, some platelet aggregates in CP/PAS showed typical apoptotic morphology, with shrinkage and membrane damage, but comparable fibrin networks were present. On day 5 however, in those units where storage conditions were uncontrolled and where the pH had decreased to below 6.4, no platelet aggregates were seen and fibrin was arranged into short, lumpy masses with no separate major or minor fibrin fibers visible. In those units stored at 22°C with continuous flat-bed agitation, where pH was maintained >7.0, ultrastructure of platelets and fibrin network in CP/PAS was typical and similar to FP and CP at the end of five days of storage. Examining platelet and fibrin network ultrastructure may be useful, in addition to conventional laboratory analysis, in assessing the viability and potential clinical efficacy of platelets for transfusion and could play a role in the evaluation of new generation platelet additive solutions.

show abstract

The New Generation of Platelet Additive Solution for Storage at 22°C: Development and Current Experience

Cited by 74 publications

References 34 publications

Morphological and functional changes in random donor platelets stored for seven days in platelet additive solution

Morphological and functional changes in random donor platelets stored for seven days in platelet additive solution

Platelets: Testing, Dosing and the Storage Lesion—Recent Advances

Investigating the effect of a platelet additive solution on apheresis platelet and fibrin network ultrastructure

Contact Info

Product

Resources

About