Morphological and functional changes in random donor platelets stored for seven days in platelet additive solution

Abstract

Introduction: Platelets are small, anucleated cytoplasmic fragments that play an essential role in blood clotting and wound healing. Platelets are routinely stored in plasma for five days at 22 0 C. Aim: Our objective was to assess platelet morphology and function in random donor platelet stored for seven days in platelet additive solution. Methods: The random donor platelet was prepared by PRP method. Platelet concentrates from 50 blood donor were stored in 100% plasma (n=24) and 20:80 (n=24) additive solution. The RDP were placed in incubator and agitator at 22°C. Results: Out of total 50 samples, 48 samples were analyzed and two were discarded due to the bacterial contamination. On comparing the mean values of platelet count and MPV in both the groups with and without platelet additive solution, no significant difference was observed on day seven of storage period. A significant difference in PDW was observed on day seven (p < 0.001) in

Ten Days Storage of Platelets: Analysis of Quality and Safety

“…This value decreased due to sampling on Day 5, which led to a decrease in weight and, consequently, a reduction in volume. This decrease in platelet count and platelet content per final unit was consistent with other studies [11][12][13][14][15].…”

“…Similarly, Singh et al [12] showed that the MPV and PDW remained constant until the 7th day of storage. However, Bashir et al [15] reported an increase in MPV over 10 days of storage from platelet-rich plasma (PRP) -PC and this correlated with another study by Chandra et al [11]. On the contrary, Baghdadi et al [16] reported that MPV and PDW decreased over 5…”

“…The platelet indices, particularly the PDW and MPV, have become essential in assessing platelet function and morphology as they reflect the degree of PSLs [11,12,16]. The PDW demonstrates the variability in platelet size, while the MPV is a value calculated by the analyser that indicates the average size of the platelets [17].…”

“…However other factors should also be considered in order to guarantee the safety of the products [9]. Cytokines in blood products can induce febrile non-haemolytic transfusion reactions (FNHTRs) or even more severe reactions such as transfusion-related acute lung injury (TRALI) and transfusion-related immunomodulation (TRIM) [8,10,11]. This study compared the platelet count and indices, variation in pH, as well as, the concentrations of cytokines; Interleukin-6 (IL-6), Tumour necrosis factor-alpha and tumour growth factor-beta on Day 5 to Day 10.…”

Platelet storage lesion: An update

“…[ 15 ] Platelet morphology and function was found to be better preserved in additive solution on day seven as compared to platelets stored without additive solution by Chandra et al . [ 16 ]…”