L-carnitine effectively improves the metabolism and quality of platelet concentrates during storage

Deyhim, Mohammad Reza; Mesbah‐Namin, Seyed Alireza; Yari, Fatemeh; Taghikhani, Mohammad; Amirizadeh, Naser

doi:10.1007/s00277-014-2243-5

Cited by 20 publications

(9 citation statements)

References 41 publications

(52 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…WST-1 test was done to unravel the mitochondrial metabolic activity and the survival rate of PLTs during storage. The survival of PLTs was decreased during storage and one of the reasons for this reduction was the production of lactate and reducing the pH (27,28). In this study, the viability was reduced in all the groups during storage time and the highest reduction in viability was observed in PLTs stored at room temperature.…”

Section: Discussionmentioning

confidence: 48%

The surface markers and survival rate of platelets during storage at 4°C: The influence of sodium octanoate

baghdadi

Yari

Rezaei

et al. 2019

IJPHO

Self Cite

View full text Add to dashboard Cite

Background: Storage of platelet concentrates (PCs) at room temperature (20-24°C) limits its storage time to 5 days due to the destructive effects of platelet storage lesion (PSL) and bacterial contamination. Although prolonged storage of platelets (PLTs) at 4°C reduces the likelihood of bacterial contamination and PSL levels, it is accompanied by an increase in the clearance rate and changes in the surface markers of PLTs. The goal of this study was to evaluate the effects of sodium octanoate (SO) as a stabilizer on PLTs during storage at 4°C. Materials and Methods: In this experimental study, PCs were divided into three portions and stored for 5 days at 3 different conditions, including 20-24°C, 4°C temperature, and 4°C in presence of SO. PLTs enumeration was performed using an automated hematology analyzer. To measure the metabolic activity and survival rate of PLTs, the water-soluble tetrazolium salt (WST-1) assay was performed. The activity of lactate dehydrogenase enzyme (LDH) was measured by a biochemical analyzer. Additionally, the levels of PLT glycoprotein Ibα (GPIbα) and CD62P (P-selectin) were measured on PLTs by flow cytometry technique. Results: PLTs count was higher in SO-treated (4°C) PLTs than two other studied samples. Additionally, the viability was higher in the SO-treated PLTs than that in other groups. LDH amount was lower in the SO-treated PLTs than that in other groups (P>0.05). GPIbα expression was significantly higher in SO-treated PLTs than that other groups (P<0.05). On the other hand, the expression of CD62P was lower at 4°C in PLTs in the presence of SO (P>0.05). Conclusions: SO could modulate the effects of cold temperatures on PLTs. Furthermore, we found that the survival of platelets was better maintained in the presence of SO at 4°C.

show abstract

Section: Discussionmentioning

confidence: 48%

The surface markers and survival rate of platelets during storage at 4°C: The influence of sodium octanoate

baghdadi

Yari

Rezaei

et al. 2019

IJPHO

Self Cite

View full text Add to dashboard Cite

show abstract

“…L‐carnitine also inhibits the apoptosis mediator Caspase‐3, suggesting it could also reduce platelet apoptosis . In vitro studies have found that addition of L‐carnitine to storage media does result in decreased lactate production and glucose consumption, with an associated preservation of pH after 2 to 5 days of storage . This is associated with an improvement in other in vitro markers of platelet function including shape change, HSR, and platelet counts after 5 days of storage, and an improved aggregation response sometime between 2 and 5 days of storage .…”

Section: Liquid Stored Plateletsmentioning

confidence: 99%

“…In vitro studies have found that addition of L‐carnitine to storage media does result in decreased lactate production and glucose consumption, with an associated preservation of pH after 2 to 5 days of storage . This is associated with an improvement in other in vitro markers of platelet function including shape change, HSR, and platelet counts after 5 days of storage, and an improved aggregation response sometime between 2 and 5 days of storage . Therefore, supplementation of platelet storage media with L‐carnitine may be beneficial for platelets stored at RT for longer than 2 days, with maximal benefit seen if RT storage is extended beyond 5 days.…”

Section: Liquid Stored Plateletsmentioning

confidence: 99%

Comprehensive review of platelet storage methods for use in the treatment of active hemorrhage

Milford

Reade

2016

Transfusion

View full text Add to dashboard Cite

This review considers the various methods currently in use, or under investigation, for the storage of platelets intended for use in the treatment of active hemorrhage. The current standard practice of storing platelets at room temperature (RT) (20°C‐24°C) optimizes circulating time, but at the expense of hemostatic function and logistical considerations. A number of alternatives are under investigation. Novel storage media additives appear to attenuate the deleterious changes that affect RT stored platelets. Cold storage was originally abandoned due to the poor circulating time of platelets stored at 4°C, but such platelets may actually be more hemostatically effective, with a number of other advantages, compared to RT stored platelets. Periodically re‐warming cold stored platelets (temperature cycling, TC) may combine the hemostatic efficacy of cold stored platelets with the longer circulating times of RT storage. Alternatives to liquid storage include cryopreservation (freezing) or lyophilization (freeze‐drying). The former has had some limited clinical use and larger clinical trials are underway, while the latter is still in the preclinical stage with promising in vitro and in vivo results. The importance of platelet transfusion in the management of active hemorrhage is now well accepted, so it is timely that platelet storage methods are reviewed with consideration of not only their circulating time, but also their hemostatic efficacy.

show abstract

“…Many physiological effects of LC have been shown. Deyhim et al demonstrated that LC as an additive improves the platelet metabolism in maintaining the quality of stored platelet concentrate for platelet transfusion therapy ( 12 ). Hollister et al reported LC leads to a modest decrease in the rate of progression of Alzheimer’s disease ( 13 ).…”

Section: Introductionmentioning

confidence: 99%

L-carnitine Effectively InduceshTERTGene Expression of Human Adipose Tissue-derived Mesenchymal Stem Cells Obtained from the Aged Subjects

Farahzadi

Mesbah‐Namin

Zarghami

et al. 2016

IJSC

Self Cite

View full text Add to dashboard Cite

Background and ObjectivesHuman mesenchymal stem cells (hMSCs) are attractive candidates for cell therapy and regenerative medicine due to their multipotency and ready availability, but their application can be complicated by the factors such as age of the donors and senescence-associated growth arrest during culture conditions. The latter most likely reflects the fact that aging of hMSCs is associated with a rise in intracellular reactive oxygen species, loss of telomerase activity, decrease in human telomerase reverse transcriptase (hTERT) expression and finally eroded telomere ends. Over-expression of telomerase in hMSCs leads to telomere elongation and may help to maintain replicative life–span of these cells. The aim of this study was to evaluate of the effect of L-carnitine (LC) as an antioxidant on the telomerase gene expression and telomere length in aged adipose tissue-derived hMSCs.MethodsFor this purpose, cells were isolated from healthy aged volunteers and their viabilities were assessed by MTT assay. Quantitative gene expression of hTERT and absolute telomere length measurement were also performed by real-time PCR in the absence and presence of different doses of LC (0.1, 0.2 and 0.4 mM).ResultsThe results indicated that LC could significantly increase the hTERT gene expression and telomere length, especially in dose of 0.2 mM of LC and in 48 h treatment for the aged adipose tissue-derived hMSCs samples.ConclusionIt seems that LC would be a good candidate to improve the lifespan of the aged adipose tissue-derived hMSCs due to over-expression of telomerase and lengthening of the telomeres.

show abstract

L-carnitine effectively improves the metabolism and quality of platelet concentrates during storage

Cited by 20 publications

References 41 publications

The surface markers and survival rate of platelets during storage at 4°C: The influence of sodium octanoate

The surface markers and survival rate of platelets during storage at 4°C: The influence of sodium octanoate

Comprehensive review of platelet storage methods for use in the treatment of active hemorrhage

L-carnitine Effectively InduceshTERTGene Expression of Human Adipose Tissue-derived Mesenchymal Stem Cells Obtained from the Aged Subjects

Contact Info

Product

Resources

About