L-carnitine Effectively InduceshTERTGene Expression of Human Adipose Tissue-derived Mesenchymal Stem Cells Obtained from the Aged Subjects

Abstract

Background and ObjectivesHuman mesenchymal stem cells (hMSCs) are attractive candidates for cell therapy and regenerative medicine due to their multipotency and ready availability, but their application can be complicated by the factors such as age of the donors and senescence-associated growth arrest during culture conditions. The latter most likely reflects the fact that aging of hMSCs is associated with a rise in intracellular reactive oxygen species, loss of telomerase activity, decrease in human telomerase reverse transcriptase (hTERT) expression and finally eroded telomere ends. Over-expression of telomerase in hMSCs leads to telomere elongation and may help to maintain replicative life–span of these cells. The aim of this study was to evaluate of the effect of L-carnitine (LC) as an antioxidant on the telomerase gene expression and telomere length in aged adipose tissue-derived hMSCs.MethodsFor this purpose, cells were isolated from healthy aged volunteers and their viabilities were assessed by MTT assay. Quantitative gene expression of hTERT and absolute telomere length measurement were also performed by real-time PCR in the absence and presence of different doses of LC (0.1, 0.2 and 0.4 mM).ResultsThe results indicated that LC could significantly increase the hTERT gene expression and telomere length, especially in dose of 0.2 mM of LC and in 48 h treatment for the aged adipose tissue-derived hMSCs samples.ConclusionIt seems that LC would be a good candidate to improve the lifespan of the aged adipose tissue-derived hMSCs due to over-expression of telomerase and lengthening of the telomeres.

Enhancement of osteogenic differentiation of rat adipose tissue-derived mesenchymal stem cells by zinc sulphate under electromagnetic field via the PKA, ERK1/2 and Wnt/β-catenin signaling pathways

“…Following incubation for 4 h, the supernatant was removed and 100 μL of dimethyl sulfoxide was added to each well. The optical density of each well was measured in an ELISA Reader (Labsystems, Helsinki, Finland) at a wavelength of 570 nm [5, 8]. …”

“…A great deal of evidence suggests that osteoporosis results from the interaction of genetic susceptibility and environmental factors [4]. Among which, zinc ion (Zn +2 ) as an essential trace element, was reported to be involved in the pathogenesis of osteoporosis and has direct effect on bone mineralization [5–8]. It has been reported that the administration of zinc sulphate (ZnSO 4 ) to weanling rats for 3 days resulted in a statistically significant increase in bone development [9].…”

Curcumin Affects Adipose Tissue-Derived Mesenchymal Stem Cell Aging Through TERT Gene Expression

“…The stock MTT dye solution (2 mg/mL) was added to each well after the plates were incubated at 37ºC in a 5 % CO 2 incubator for 4 h. Following incubation for 4 h, the supernatant was removed and 100 µL of dimethyl sulfoxide was added to each well. The optical density of each well was measured in an ELISA Reader (Labsystems, Helsinki, Finland) at a wavelength of 570 nm [19].…”

Emerging Importance of Phytochemicals in Regulation of Stem Cells Fate via Signaling Pathways

“…Stem cells are uncommitted cells that have potential to differentiate into specialized cell types (Nejati‐Koshki et al ., ). They are able to maintain renewal activity by asymmetric cell divisions (Nejati‐Koshki et al ., ; Farahzadi et al ., ). Also, stem cells have opened new era in the field of developmental biology and regenerative medicine.…”