The New Biomarker for Cervical Squamous Cell Carcinoma and Endocervical Adenocarcinoma (CESC) Based on Public Database Mining

Ding, Hao; Xiong, Xiao-Xing; Fan, Guanlan; Yi, Yuexiong; Chen, Yurou; Wang, Jingtao; Zhang, Wei

doi:10.1155/2020/5478574

Cited by 19 publications

(12 citation statements)

References 48 publications

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“…HCAR3 is a receptor for 3-OH-octanoid acid and a low affinity receptor for nicotinic acid, which mediates the negative feedback regulation of adipocyte lipolysis [ 13 ]. Previous studies reported that higher expression of HCAR3 was negatively correlated with survival time of cervical cancer patients [ 14 ]. In CRC patients, the average expression of HCAR3 was increased in CRC tissues compared to normal tissues (Fig.…”

Section: Resultsmentioning

confidence: 99%

Identification and verification of HCAR3 and INSL5 as new potential therapeutic targets of colorectal cancer

et al. 2021

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Background Colorectal cancer (CRC) is one of the most common cancers of the gastrointestinal tract and ranks third in cancer-related deaths worldwide. This study was conducted to identify novel biomarkers related to the pathogenesis of CRC based upon a bioinformatics analysis, and further verify the biomarkers in clinical tumor samples and CRC cell lines. Methods A series of bioinformatics analyses were performed using datasets from NCBI-GEO and constructed a protein–protein interaction (PPI) network. This analysis enabled the identification of Hub genes, for which the mRNA expression and overall survival of CRC patients data distribution was explored in The Cancer Genome Atlas (TCGA) colon cancer and rectal cancer (COADREAD) database. Furthermore, the differential expression of HCAR3 and INLS5 was validated in clinical tumor samples by Real-time quantitative PCR analysis, western blotting analysis, and immunohistochemistry analysis. Finally, CRC cells over-expressing INSL5 were constructed and used for CCK8, cell cycle, and cell apoptosis validation assays in vitro. Results A total of 286 differentially expressed genes (DEGs) were screened, including 64 genes with increased expression and 143 genes with decreased expression in 2 CRC database, from which 10 key genes were identified: CXCL1, HCAR3, CXCL6, CXCL8, CXCL2, CXCL5, PPY, SST, INSL5, and NPY1R. Among these genes, HCAR3 and INSL5 had not previously been explored and were further verified in vitro. Conclusions HCAR3 expression was higher in CRC tissues and associated with better overall survival of CRC patients. INSL5 expression in normal tissue was higher than that in tumor tissue and its high expression was associated with a better prognosis for CRC. The overexpression of INSL5 significantly inhibited the proliferation and promoted the shearing of PARP of CRC cells. This integrated bioinformatics study presented 10 key hub genes associated with CRC. HCAR3 and INSL5 were expressed in tumor tissue and these were associated with poor survival and warrant further studies as potential therapeutic targets.

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Section: Resultsmentioning

confidence: 99%

Identification and verification of HCAR3 and INSL5 as new potential therapeutic targets of colorectal cancer

et al. 2021

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“…Gynecological cancer is a serious threat to women’s health worldwide, especially cervical squamous cell carcinoma and endocervical adenocarcinoma (CESC), ovarian cancer (OV), uterine corpus endometrial carcinoma (UCEC), and uterine carcinosarcoma (UCS). Globally, CESC is the fourth most common female malignancy and the second-highest cause of female cancer-related deaths [ 1 , 2 ]. Human papillomavirus infection (HPV) is the main cause of cervical cancer.…”

Section: Introductionmentioning

confidence: 99%

Tumor purity as a prognosis and immunotherapy relevant feature in cervical cancer

Deng

Song

Huang

et al. 2021

Aging

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Background: Tumor purity plays a vital role in the biological process of solid tumors, but its function in gynecologic cancers remains unclear. This study explored the correlation between tumor purity and immune function of gynecological cancers and its reliability as a prognostic indicator of immunotherapy. Methods: Gynecological cancer-related datasets were downloaded from The Cancer Genome Atlas (TCGA). Tumor purity was calculated by the ESTIMATE algorithm. A LASSO Cox regression analysis was performed to construct the risk score model. A Kaplan–Meier Plotter was used to explore the relationships between tumor purity and cancer prognosis. We performed the Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Set Enrichment Analysis (GSEA) to explore the pathways in the subgroups. A nomogram was used to quantitatively assess the cancer prognosis. Results: Tumor purity was negatively correlated with B cell infiltration in cervical squamous cell carcinoma and endocervical adenocarcinoma (CESC). Approximately 420 genes were positively associated with B cell infiltration and CESC prognosis and were enriched in immune-related signaling pathways. There were 11 key genes used to construct a risk score model. The low-risk group had a higher immune score and better prognosis than the high-risk group. A nomogram based on risk score, T stage, and clinical-stage had good predictive value in quantitatively evaluating CESC prognosis. Conclusions: This study is the first to reveal the correlation between tumor purity and immunity in CESC and suggests that low-risk patients may be more sensitive to immunotherapy. This provides a theoretical basis for the clinical treatment of CESC.

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“…The modern methods are used to search for serum markers in cervical cancer by mean of perspective technologies such as magnetic bead-based weak cation-exchange chromatography fractionation combined with matrix-assisted laser desorption / ionization-time of flight mass spectrometry [100], the multiplex proximity extension assay is used [101]. A significant amount of modern research of cervical neoplasia is aimed at identifying genetic, epigenetic changes, revealing the possible role of long non-coding RNAs and circular RNAs in cervical carcinogenesis [102][103][104][105][106][107]. Modern tendencies in the problem of cervical neoplasia include research on immune factors and the vaginal microbiome [108][109][110].…”

Section: Discussionmentioning

confidence: 99%

Cervical Carcinoma: Oncobiology and Biomarkers

Volkova

Pashov

Omelchuk

2021

IJMS

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Cervical cancer is one of the most common types of carcinomas causing morbidity and mortality in women in all countries of the world. At the moment, the oncology, oncobiology, and oncomorphology of cervical cancer are characterized by the accumulation of new information; various molecular biological, genetic, and immunohistochemical methods of investigation of the mechanisms of cervical carcinogenesis are tested and applied; targeted antitumour drugs and diagnostic, prognostic, and predictive biomarkers are being searched for. Many issues of the etiopathogenesis of cervical cancer have not been sufficiently studied, and the role of many biomarkers characterizing various stages of cervical carcinogenesis remains unclear. Therefore, the target of this review is to systematize and understand several problems in the pathogenesis of cervical cancer and to evaluate the significance and role of biomarkers in cervical carcinogenesis.

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The New Biomarker for Cervical Squamous Cell Carcinoma and Endocervical Adenocarcinoma (CESC) Based on Public Database Mining

Cited by 19 publications

References 48 publications

Identification and verification of HCAR3 and INSL5 as new potential therapeutic targets of colorectal cancer

Identification and verification of HCAR3 and INSL5 as new potential therapeutic targets of colorectal cancer

Tumor purity as a prognosis and immunotherapy relevant feature in cervical cancer

Cervical Carcinoma: Oncobiology and Biomarkers

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