The need for standardized bloodspot TSH-calibrators in congenital hypothyroidism screening

Elvers, L.H.; Loeber, J.G.

doi:10.1016/0378-3782(96)01760-4

Cited by 7 publications

(3 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Infant blood was collected on dried blood spot cards at postnatal days 7, 14, 28 and 34 weeks’ gestational age (had the fetus remained in utero, referred to hereafter as equivalent gestational age), ±1 day. Cards were sent to the Amsterdam Neonatal Screening Laboratory for measurement of TSH, T4 and TBG18 (for details see online supplementary appendix). T4 is expressed as absolute levels and as a SD of the daily mean, which is calculated from the results of approximately 150 cards of term and preterm infants; levels ≤−3.0 SD are considered abnormal (in term infants).…”

Section: Methodsmentioning

confidence: 99%

Thyroid function in preterm infants and neurodevelopment at 2 years

Williams

Lindgren

Watson

et al. 2020

Arch Dis Child Fetal Neonatal Ed

View full text Add to dashboard Cite

ObjectivesPostnatal thyroid dysfunction is common in preterm infants but the relationship between mild dysfunction and neurodevelopment is unclear. Our aim is to describe the relationship between thyroid function and neurodevelopment.DesignCohort analysis.Patients1275 infants born under 31 weeks’ gestation; there were no exclusion criteria.SettingThe infants were part of a UK daily iodine supplementation trial.Main outcomesThyroid-stimulating hormone, thyroid-binding globulin and total thyroxine levels were measured in dried blood spots on postnatal days 7, 14, 28 and the equivalent of 34 weeks’ gestation. Neurodevelopment was measured using the Bayley-III Scales of infant development at 2 years of age.ResultsNo infant was identified as hypothyroid through routine screening. The 3% of infants consistently in the top decile of gestationally age-adjusted thyroid-stimulating hormone levels had a reduction in cognitive score of 7 Bayley units when compared with those not in the top decile (95% CI –13 to –1). A reduction in motor composite score of 6 units (95% CI −12 to <−0.1) and fine motor score of 1 unit (95% CI –2 to –0.1) was also identified. The 0.7% of infants consistently in the bottom decile of age-adjusted thyroxine levels had a reduction in motor composite score of 14 units (95% CI –25 to –2) and its two subset scores, fine and gross motor, of 2 units (95% CI respectively −4.5 to <−0.1 and –4.3 to –0.3).ConclusionsPreterm infants with consistent ‘mild’ thyroid dysfunction score less on neurodevelopmental tests at 2 years of age. Many of these infants will not be detected by current clinical protocols or screening programmes.

show abstract

Section: Methodsmentioning

confidence: 99%

Thyroid function in preterm infants and neurodevelopment at 2 years

Williams

Lindgren

Watson

et al. 2020

Arch Dis Child Fetal Neonatal Ed

View full text Add to dashboard Cite

show abstract

“…Screening parameters such as sensitivity and specificity cannot be compared between programmes without discussion of cut-off values, and this is problematic because of highly variable numeric results given by different test kits, even when pure or international reference material is available for calibrator production (Elvers and Loeber 1996) The use of regional standard materials for phenylalanine measurement has been shown to improve this situation (Dhondt et al 1996(Dhondt et al , 1998 It has been shown that this type of material (for phenylalanine and TSH) can be reliably produced over time and between centres (Adam et al 2000). Standards have been produced for these analytes and 17-hydroxyprogesterone (17-OHP) (Elvers et al 2005(Elvers et al , 2006 Unfortunately adoption of the values on the international materials would necessitate changes by some manufacturers of their kit values and some fear repercussions from their customers were they to do this, so the international screening community has been slow in adopting this particular standardization.…”

Section: Laboratory Qualitymentioning

confidence: 99%

Quality performance of newborn screening systems: Strategies for improvement

Webster¹

2007

J of Inher Metab Disea

View full text Add to dashboard Cite

Newborn metabolic screening is a public health activity with the potential to realize significant health gains for infants affected with a range of congenital conditions. Many of these are inborn errors of metabolism. The activities required to achieve the gains are diverse and carried out by a number of organizations, by families and by many health care professionals. Laboratories have the best-developed quality strategies, which include quality assurance programmes, guidelines, protocols and standards. Two-tier testing and use of multiple markers improve sensitivity and specificity. There are international initiatives to harmonize assay materials and definitions to allow better benchmarking between programmes. Outside the laboratory, standards, education and protocols improve the quality of specimen collection, diagnosis and treatment, which together produce the health gains.

show abstract

“…La concentración del analito medida en manchas de sangre neonatal depende de la forma en que son preparados los calibradores. Muchas variables están involucradas en este proceso; tales como el tipo y el lote del papel de filtro usado, la matriz de los calibradores, el porcentaje del hematocrito, el tamaño de la mancha de sangre, el uso de células rojas lisadas o intactas, el uso de anticoagulantes y bactericidas, las condiciones de almacenamiento y el secado, la preparación de referencia usada para establecer la concentración de los calibradores y el método de análisis usado (4).…”

Section: Introduccionunclassified

Estudio comparativo de los tres papeles de filtro en los ensayos UMELISA para el tamizaje neonatal de hipotiroidismo congénito y fenilcetonuria

et al. 2002

View full text Add to dashboard Cite

Introducción. El tamizaje neonatal de Hipotiroidismo Congénito y otras enfermedades heredometabólicas comparten el hecho de que los analitos de interés son determinados en manchas de sangre seca colectada en papel de filtro. La concentración del analito depende de la forma en que son preparados los calibradores y de otras variables que están involucradas en este proceso; como el lote y el tipo de papel de filtro usado, la matriz de los calibradores, el porcentaje de hematocrito entre otros. Material y métodos. Se evaluaron 4 controles preparados en sangre total y ajustados al 55% de hematocrito con diferentes niveles de concentración de TSH, T4 y Fenilalanina cada control fue dispensado en tarjetas de papel de filtro Whatman BFC 180, S&S 2992 y 903, las concentraciones de cada analito se midieron utilizando los UMELISAS TSH y T4 Neonatal y en el UMTEST PKU. Se realizó un estudio de estabilidad durante 9 meses, para los controles conservados entre 2-8°C, además fueron evaluadas 400 muestras de neonatos colectadas simultáneamente en papel de filtro 2992 y 903. Resultados y conclusiones. La aplicación de la prueba t para datos pareados demostró que no existen diferencias estadísticamente significativas entre los tres tipos de papel de filtro para un intervalo de confianza del 95% al no producir afectación en la precisión y exactitud de estos métodos. El porcentaje de pérdida de actividad de los 3 analitos estudiados fue inferior al 17%. Los valores promedios de concentración en la distribución de TSH y Phe son ligeramente mayores en las muestras colectadas en papel 903.

show abstract

The need for standardized bloodspot TSH-calibrators in congenital hypothyroidism screening

Cited by 7 publications

References 1 publication

Thyroid function in preterm infants and neurodevelopment at 2 years

Thyroid function in preterm infants and neurodevelopment at 2 years

Quality performance of newborn screening systems: Strategies for improvement

Estudio comparativo de los tres papeles de filtro en los ensayos UMELISA para el tamizaje neonatal de hipotiroidismo congénito y fenilcetonuria

Contact Info

Product

Resources

About